Transfer and functional consequences of dietary microRNAs in vertebrates: concepts in search of corroboration: negative results challenge the hypothesis that dietary xenomiRs cross the gut and regulate genes in ingesting vertebrates, but important questions persist.
Bottom Line: RNA interference (RNAi) mechanisms of Caenorhabditis elegans are enhanced by uptake of environmental RNA and amplification and systemic distribution of RNAi effectors.In this article, we review the evidence for and against a significant role for dietary miRNAs in influencing gene expression, and make recommendations for future studies.Also watch the Video Abstract.
Affiliation: Department of Molecular and Comparative Pathobiology, The Johns Hopkins University, Baltimore, MD, USA.Show MeSH
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Mentions: Given the instability of naked RNA, transcytosis – vesicular uptake of RNA carriers and their cargo (Fig. 3A) on one side and release on the other side of a biological barrier – is an attractive option (Fig. 3B). Alternatively, uptake into the cytoplasm by transmembrane RNA transporters is hypothetically possible (Fig. 3C) but would require close contact with a cell or carrier that releases the RNA in the immediate vicinity of the transporter. Additionally, immune cells could take up RNA/RNA carriers and release them on the other side of the barrier (Fig. 3D).
Affiliation: Department of Molecular and Comparative Pathobiology, The Johns Hopkins University, Baltimore, MD, USA.