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Transfer and functional consequences of dietary microRNAs in vertebrates: concepts in search of corroboration: negative results challenge the hypothesis that dietary xenomiRs cross the gut and regulate genes in ingesting vertebrates, but important questions persist.

Witwer KW, Hirschi KD - Bioessays (2014)

Bottom Line: RNA interference (RNAi) mechanisms of Caenorhabditis elegans are enhanced by uptake of environmental RNA and amplification and systemic distribution of RNAi effectors.In this article, we review the evidence for and against a significant role for dietary miRNAs in influencing gene expression, and make recommendations for future studies.Also watch the Video Abstract.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular and Comparative Pathobiology, The Johns Hopkins University, Baltimore, MD, USA.

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Related in: MedlinePlus

Variability of apparent xenomiR detection in human sera: reanalysis of Supplementary Data from Zhang et al. Four plant miRNAs were detected in each of 10 human serum pools examined in the original dietary miRNA study [3]. Pools were composed of 10–11 individuals each, with no assignment criteria specified (other than gender for the two indicated pools). There was substantial variability between pools. Shown are reads per million animal miRNAs for each pool, followed on the right of each panel by a 95% confidence interval for all miRNA-specific data.
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fig02: Variability of apparent xenomiR detection in human sera: reanalysis of Supplementary Data from Zhang et al. Four plant miRNAs were detected in each of 10 human serum pools examined in the original dietary miRNA study [3]. Pools were composed of 10–11 individuals each, with no assignment criteria specified (other than gender for the two indicated pools). There was substantial variability between pools. Shown are reads per million animal miRNAs for each pool, followed on the right of each panel by a 95% confidence interval for all miRNA-specific data.

Mentions: Pooling samples can be an effective strategy in certain circumstances but will also mask biological variability. In Zhang et al., though, plant miRNA reads in ten serum pools derived from 10 to 11 donors each varied strikingly [3,53] (Fig. 2). MIR168a reads varied >2,000-fold. The necessarily even more profound variability at the sample level does not seem reconcilable with a general biological phenomenon and would be more easily explained by other factors:


Transfer and functional consequences of dietary microRNAs in vertebrates: concepts in search of corroboration: negative results challenge the hypothesis that dietary xenomiRs cross the gut and regulate genes in ingesting vertebrates, but important questions persist.

Witwer KW, Hirschi KD - Bioessays (2014)

Variability of apparent xenomiR detection in human sera: reanalysis of Supplementary Data from Zhang et al. Four plant miRNAs were detected in each of 10 human serum pools examined in the original dietary miRNA study [3]. Pools were composed of 10–11 individuals each, with no assignment criteria specified (other than gender for the two indicated pools). There was substantial variability between pools. Shown are reads per million animal miRNAs for each pool, followed on the right of each panel by a 95% confidence interval for all miRNA-specific data.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109825&req=5

fig02: Variability of apparent xenomiR detection in human sera: reanalysis of Supplementary Data from Zhang et al. Four plant miRNAs were detected in each of 10 human serum pools examined in the original dietary miRNA study [3]. Pools were composed of 10–11 individuals each, with no assignment criteria specified (other than gender for the two indicated pools). There was substantial variability between pools. Shown are reads per million animal miRNAs for each pool, followed on the right of each panel by a 95% confidence interval for all miRNA-specific data.
Mentions: Pooling samples can be an effective strategy in certain circumstances but will also mask biological variability. In Zhang et al., though, plant miRNA reads in ten serum pools derived from 10 to 11 donors each varied strikingly [3,53] (Fig. 2). MIR168a reads varied >2,000-fold. The necessarily even more profound variability at the sample level does not seem reconcilable with a general biological phenomenon and would be more easily explained by other factors:

Bottom Line: RNA interference (RNAi) mechanisms of Caenorhabditis elegans are enhanced by uptake of environmental RNA and amplification and systemic distribution of RNAi effectors.In this article, we review the evidence for and against a significant role for dietary miRNAs in influencing gene expression, and make recommendations for future studies.Also watch the Video Abstract.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular and Comparative Pathobiology, The Johns Hopkins University, Baltimore, MD, USA.

Show MeSH
Related in: MedlinePlus