Transfer and functional consequences of dietary microRNAs in vertebrates: concepts in search of corroboration: negative results challenge the hypothesis that dietary xenomiRs cross the gut and regulate genes in ingesting vertebrates, but important questions persist.
Bottom Line: RNA interference (RNAi) mechanisms of Caenorhabditis elegans are enhanced by uptake of environmental RNA and amplification and systemic distribution of RNAi effectors.In this article, we review the evidence for and against a significant role for dietary miRNAs in influencing gene expression, and make recommendations for future studies.Also watch the Video Abstract.
Affiliation: Department of Molecular and Comparative Pathobiology, The Johns Hopkins University, Baltimore, MD, USA.Show MeSH
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Mentions: Pooling samples can be an effective strategy in certain circumstances but will also mask biological variability. In Zhang et al., though, plant miRNA reads in ten serum pools derived from 10 to 11 donors each varied strikingly [3,53] (Fig. 2). MIR168a reads varied >2,000-fold. The necessarily even more profound variability at the sample level does not seem reconcilable with a general biological phenomenon and would be more easily explained by other factors:
Affiliation: Department of Molecular and Comparative Pathobiology, The Johns Hopkins University, Baltimore, MD, USA.