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Assay for Glycosaminoglycans by Tandem Mass Spectrometry and its Applications.

Tomatsu S, Shimada T, Mason RW, Kelly J, LaMarr WA, Yasuda E, Shibata Y, Futatsumori H, Montaño AM, Yamaguchi S, Suzuki Y, Orii T - J Anal Bioanal Tech (2014)

Bottom Line: The RF system consists of an integrated solid phase extraction robot that binds and de-salts samples from assay plates and directly injects them into a MS/MS detector, reducing sample processing time to ten seconds.However, the RF system does not have a chromatographic step, and therefore, cannot distinguish GAGs that have identical molecular weights.In this article, we compare the assay methods for GAGs and describe their potential applications.

View Article: PubMed Central - PubMed

Affiliation: Nemours/Alfred I. duPont Hospital for Children, Wilmington, DE, USA.

ABSTRACT
Glycosaminoglycans (GAGs) are distributed in the whole body and play a variety of important physiological roles associated with inflammation, growth, coagulation, fibrinolysis, lipolysis, and cell-matrix biology. Accumulation of undegraded GAGs in lysosomes gives rise to a distinct clinical syndrome, mucopolysaccharidoses. Measurement of each specific GAG in a variety of specimens is urgently required to understand GAG interaction with other molecules, physiological status of patients, and prognosis and pathogenesis of the disease. We established a highly sensitive and accurate tandem mass spectrometry (LC-MS/MS) method for measurements of disaccharides derived from four specific GAGs [dermatan sulfate (DS), heparan sulfate (HS), keratan sulfate (KS), and chondroitin sulfate (CS)]. Disaccharides were produced by specific enzyme digestion of each GAG, and quantified by negative ion mode of multiple reaction monitoring. Subclasses of HS and GAGs with identical molecular weights can be separated using a Hypercarbcolumn (2.0 mm×50 mm, 5 μm) with an aectonitrile gradient in ammonium acetate (pH 11.0). We also developed a GAG assay by RapidFire with tandem mass spectrometry (RF-MS/MS). The RF system consists of an integrated solid phase extraction robot that binds and de-salts samples from assay plates and directly injects them into a MS/MS detector, reducing sample processing time to ten seconds. RF-MS/MS consequently yields much faster throughput than conventional LC-MS/MS-based methods. However, the RF system does not have a chromatographic step, and therefore, cannot distinguish GAGs that have identical molecular weights. Both methods can be applied to analysis of dried blood spots, blood, and urine specimens. In this article, we compare the assay methods for GAGs and describe their potential applications.

No MeSH data available.


Related in: MedlinePlus

Correlation in GAG concentration between LC-MS/MS and RF-MS/MSA. ΔDiHS-NS concentration. Correlation in blood (plasma or serum) ΔDiHS-NS of human subjects between LC-MS/MS and RF-MS/MS is significant (n=187, p<0.0001). B. ΔDiHS-0S concentration. Correlation in blood (plasma or serum) ΔDiHS-0S of human subjects between LC-MS/MS and RF-MS/MS is significant (n=187, p<0.0001).
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Figure 9: Correlation in GAG concentration between LC-MS/MS and RF-MS/MSA. ΔDiHS-NS concentration. Correlation in blood (plasma or serum) ΔDiHS-NS of human subjects between LC-MS/MS and RF-MS/MS is significant (n=187, p<0.0001). B. ΔDiHS-0S concentration. Correlation in blood (plasma or serum) ΔDiHS-0S of human subjects between LC-MS/MS and RF-MS/MS is significant (n=187, p<0.0001).

Mentions: We analyzed 59 different plasma samples for DiHS-NS, DiHS-0S, Di-4S, and KS by both LC-MS/MS and RF-MS/MS. The correlation between LC-MS/MS and RF-MS/MS determinations was tested by a simple linear regression analysis. We interpreted correlation strength as outlined by Johnston [23]. Briefly, correlations are interpreted based on r values as follows: 0.0 to 0.2, very weak to negligible correlation; 0.2 to 0.4, weak correlation; 0.4 to 0.7, moderate correlation; 0.7 to 0.9, strong correlation. Data points that were greater than three standard deviations from the mean for each assay were considered outliers. Analysis was performed using SPSS for Windows (version 17.0, SPSS Inc., Chicago, IL, USA). The moderate correlation in serum ΔDiHS-NS and ΔDiHS-0S measurements of control subjects between LC-MS/MS and RF-MS/MS indicates that results from each assay are comparable (Figure 9). Measurements of KS also showed moderate correlation (data not shown).


Assay for Glycosaminoglycans by Tandem Mass Spectrometry and its Applications.

Tomatsu S, Shimada T, Mason RW, Kelly J, LaMarr WA, Yasuda E, Shibata Y, Futatsumori H, Montaño AM, Yamaguchi S, Suzuki Y, Orii T - J Anal Bioanal Tech (2014)

Correlation in GAG concentration between LC-MS/MS and RF-MS/MSA. ΔDiHS-NS concentration. Correlation in blood (plasma or serum) ΔDiHS-NS of human subjects between LC-MS/MS and RF-MS/MS is significant (n=187, p<0.0001). B. ΔDiHS-0S concentration. Correlation in blood (plasma or serum) ΔDiHS-0S of human subjects between LC-MS/MS and RF-MS/MS is significant (n=187, p<0.0001).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109812&req=5

Figure 9: Correlation in GAG concentration between LC-MS/MS and RF-MS/MSA. ΔDiHS-NS concentration. Correlation in blood (plasma or serum) ΔDiHS-NS of human subjects between LC-MS/MS and RF-MS/MS is significant (n=187, p<0.0001). B. ΔDiHS-0S concentration. Correlation in blood (plasma or serum) ΔDiHS-0S of human subjects between LC-MS/MS and RF-MS/MS is significant (n=187, p<0.0001).
Mentions: We analyzed 59 different plasma samples for DiHS-NS, DiHS-0S, Di-4S, and KS by both LC-MS/MS and RF-MS/MS. The correlation between LC-MS/MS and RF-MS/MS determinations was tested by a simple linear regression analysis. We interpreted correlation strength as outlined by Johnston [23]. Briefly, correlations are interpreted based on r values as follows: 0.0 to 0.2, very weak to negligible correlation; 0.2 to 0.4, weak correlation; 0.4 to 0.7, moderate correlation; 0.7 to 0.9, strong correlation. Data points that were greater than three standard deviations from the mean for each assay were considered outliers. Analysis was performed using SPSS for Windows (version 17.0, SPSS Inc., Chicago, IL, USA). The moderate correlation in serum ΔDiHS-NS and ΔDiHS-0S measurements of control subjects between LC-MS/MS and RF-MS/MS indicates that results from each assay are comparable (Figure 9). Measurements of KS also showed moderate correlation (data not shown).

Bottom Line: The RF system consists of an integrated solid phase extraction robot that binds and de-salts samples from assay plates and directly injects them into a MS/MS detector, reducing sample processing time to ten seconds.However, the RF system does not have a chromatographic step, and therefore, cannot distinguish GAGs that have identical molecular weights.In this article, we compare the assay methods for GAGs and describe their potential applications.

View Article: PubMed Central - PubMed

Affiliation: Nemours/Alfred I. duPont Hospital for Children, Wilmington, DE, USA.

ABSTRACT
Glycosaminoglycans (GAGs) are distributed in the whole body and play a variety of important physiological roles associated with inflammation, growth, coagulation, fibrinolysis, lipolysis, and cell-matrix biology. Accumulation of undegraded GAGs in lysosomes gives rise to a distinct clinical syndrome, mucopolysaccharidoses. Measurement of each specific GAG in a variety of specimens is urgently required to understand GAG interaction with other molecules, physiological status of patients, and prognosis and pathogenesis of the disease. We established a highly sensitive and accurate tandem mass spectrometry (LC-MS/MS) method for measurements of disaccharides derived from four specific GAGs [dermatan sulfate (DS), heparan sulfate (HS), keratan sulfate (KS), and chondroitin sulfate (CS)]. Disaccharides were produced by specific enzyme digestion of each GAG, and quantified by negative ion mode of multiple reaction monitoring. Subclasses of HS and GAGs with identical molecular weights can be separated using a Hypercarbcolumn (2.0 mm×50 mm, 5 μm) with an aectonitrile gradient in ammonium acetate (pH 11.0). We also developed a GAG assay by RapidFire with tandem mass spectrometry (RF-MS/MS). The RF system consists of an integrated solid phase extraction robot that binds and de-salts samples from assay plates and directly injects them into a MS/MS detector, reducing sample processing time to ten seconds. RF-MS/MS consequently yields much faster throughput than conventional LC-MS/MS-based methods. However, the RF system does not have a chromatographic step, and therefore, cannot distinguish GAGs that have identical molecular weights. Both methods can be applied to analysis of dried blood spots, blood, and urine specimens. In this article, we compare the assay methods for GAGs and describe their potential applications.

No MeSH data available.


Related in: MedlinePlus