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Nitric oxide-releasing porous silicon nanoparticles.

Kafshgari MH, Cavallaro A, Delalat B, Harding FJ, McInnes SJ, Mäkilä E, Salonen J, Vasilev K, Voelcker NH - Nanoscale Res Lett (2014)

Bottom Line: Subsequent reduction of nitrite in the presence of d-glucose led to the production of large NO payloads without reducing the biocompatibility of the PSi NPs with mammalian cells.The resulting PSi NPs demonstrated sustained release of NO and showed remarkable antibacterial efficiency and anti-biofilm-forming properties.These results will set the stage to develop antimicrobial nanoparticle formulations for applications in chronic wound treatment.

View Article: PubMed Central - HTML - PubMed

Affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Mawson Institute, University of South Australia, GPO Box 2471 Adelaide, SA 5001, Australia.

ABSTRACT
In this study, the ability of porous silicon nanoparticles (PSi NPs) to entrap and deliver nitric oxide (NO) as an effective antibacterial agent is tested against different Gram-positive and Gram-negative bacteria. NO was entrapped inside PSi NPs functionalized by means of the thermal hydrocarbonization (THC) process. Subsequent reduction of nitrite in the presence of d-glucose led to the production of large NO payloads without reducing the biocompatibility of the PSi NPs with mammalian cells. The resulting PSi NPs demonstrated sustained release of NO and showed remarkable antibacterial efficiency and anti-biofilm-forming properties. These results will set the stage to develop antimicrobial nanoparticle formulations for applications in chronic wound treatment.

No MeSH data available.


Related in: MedlinePlus

Toxicity of the NPs to NIH/3T3 fibroblasts using the LDH assay after48-h incubationc NO/THCPSi NPs (red bars), glucose/THCPSi NPs(blue bars), and THCPSi NPs (yellow bars). Viability measures normalizedto no NP control samples (n = 3;mean ± standard deviation shown).
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Figure 6: Toxicity of the NPs to NIH/3T3 fibroblasts using the LDH assay after48-h incubationc NO/THCPSi NPs (red bars), glucose/THCPSi NPs(blue bars), and THCPSi NPs (yellow bars). Viability measures normalizedto no NP control samples (n = 3;mean ± standard deviation shown).

Mentions: The biocompatibility of THCPSi NPs has been previously reported by Santos andco-workers [25,28], where cytotoxicity, oxidative, and inflammatory responses werestudied for a variety of mammalian cell lines. The toxicity of NO/THCPSi NPs,glucose/THCPSi NPs, and THCPSi NPs at different concentrations (0.05 to0.2 mg/mL) over 48 h was evaluated using the NIH/3T3 cell line, whichis one of the most commonly used fibroblast cell lines and often used as a modelfor skin cells. Two viability assays were used for toxicity studies: LDH andfluorescein diacetate-propidium iodide (FDA-PI). As shown in Figure 6, the results from the LDH assay showed well over 90%viability for all NP types up to 0.1 mg/mL. However, increasing theconcentration of NO/THCPSi NPs to 0.2 mg/mL reduced the viability ofNIH/3T3 cells to 92%. In contrast, the viability of fibroblast cells incubatedwith glucose/THCPSi NPs and THCPSi NPs at 0.15 and 0.2 mg/mL remained over95%. The results of the FDA-PI assay (Additional file 1: Figure S3) were consistent with those obtained using the LDHassay.


Nitric oxide-releasing porous silicon nanoparticles.

Kafshgari MH, Cavallaro A, Delalat B, Harding FJ, McInnes SJ, Mäkilä E, Salonen J, Vasilev K, Voelcker NH - Nanoscale Res Lett (2014)

Toxicity of the NPs to NIH/3T3 fibroblasts using the LDH assay after48-h incubationc NO/THCPSi NPs (red bars), glucose/THCPSi NPs(blue bars), and THCPSi NPs (yellow bars). Viability measures normalizedto no NP control samples (n = 3;mean ± standard deviation shown).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109794&req=5

Figure 6: Toxicity of the NPs to NIH/3T3 fibroblasts using the LDH assay after48-h incubationc NO/THCPSi NPs (red bars), glucose/THCPSi NPs(blue bars), and THCPSi NPs (yellow bars). Viability measures normalizedto no NP control samples (n = 3;mean ± standard deviation shown).
Mentions: The biocompatibility of THCPSi NPs has been previously reported by Santos andco-workers [25,28], where cytotoxicity, oxidative, and inflammatory responses werestudied for a variety of mammalian cell lines. The toxicity of NO/THCPSi NPs,glucose/THCPSi NPs, and THCPSi NPs at different concentrations (0.05 to0.2 mg/mL) over 48 h was evaluated using the NIH/3T3 cell line, whichis one of the most commonly used fibroblast cell lines and often used as a modelfor skin cells. Two viability assays were used for toxicity studies: LDH andfluorescein diacetate-propidium iodide (FDA-PI). As shown in Figure 6, the results from the LDH assay showed well over 90%viability for all NP types up to 0.1 mg/mL. However, increasing theconcentration of NO/THCPSi NPs to 0.2 mg/mL reduced the viability ofNIH/3T3 cells to 92%. In contrast, the viability of fibroblast cells incubatedwith glucose/THCPSi NPs and THCPSi NPs at 0.15 and 0.2 mg/mL remained over95%. The results of the FDA-PI assay (Additional file 1: Figure S3) were consistent with those obtained using the LDHassay.

Bottom Line: Subsequent reduction of nitrite in the presence of d-glucose led to the production of large NO payloads without reducing the biocompatibility of the PSi NPs with mammalian cells.The resulting PSi NPs demonstrated sustained release of NO and showed remarkable antibacterial efficiency and anti-biofilm-forming properties.These results will set the stage to develop antimicrobial nanoparticle formulations for applications in chronic wound treatment.

View Article: PubMed Central - HTML - PubMed

Affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Mawson Institute, University of South Australia, GPO Box 2471 Adelaide, SA 5001, Australia.

ABSTRACT
In this study, the ability of porous silicon nanoparticles (PSi NPs) to entrap and deliver nitric oxide (NO) as an effective antibacterial agent is tested against different Gram-positive and Gram-negative bacteria. NO was entrapped inside PSi NPs functionalized by means of the thermal hydrocarbonization (THC) process. Subsequent reduction of nitrite in the presence of d-glucose led to the production of large NO payloads without reducing the biocompatibility of the PSi NPs with mammalian cells. The resulting PSi NPs demonstrated sustained release of NO and showed remarkable antibacterial efficiency and anti-biofilm-forming properties. These results will set the stage to develop antimicrobial nanoparticle formulations for applications in chronic wound treatment.

No MeSH data available.


Related in: MedlinePlus