Limits...
Nitric oxide-releasing porous silicon nanoparticles.

Kafshgari MH, Cavallaro A, Delalat B, Harding FJ, McInnes SJ, Mäkilä E, Salonen J, Vasilev K, Voelcker NH - Nanoscale Res Lett (2014)

Bottom Line: Subsequent reduction of nitrite in the presence of d-glucose led to the production of large NO payloads without reducing the biocompatibility of the PSi NPs with mammalian cells.The resulting PSi NPs demonstrated sustained release of NO and showed remarkable antibacterial efficiency and anti-biofilm-forming properties.These results will set the stage to develop antimicrobial nanoparticle formulations for applications in chronic wound treatment.

View Article: PubMed Central - HTML - PubMed

Affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Mawson Institute, University of South Australia, GPO Box 2471 Adelaide, SA 5001, Australia.

ABSTRACT
In this study, the ability of porous silicon nanoparticles (PSi NPs) to entrap and deliver nitric oxide (NO) as an effective antibacterial agent is tested against different Gram-positive and Gram-negative bacteria. NO was entrapped inside PSi NPs functionalized by means of the thermal hydrocarbonization (THC) process. Subsequent reduction of nitrite in the presence of d-glucose led to the production of large NO payloads without reducing the biocompatibility of the PSi NPs with mammalian cells. The resulting PSi NPs demonstrated sustained release of NO and showed remarkable antibacterial efficiency and anti-biofilm-forming properties. These results will set the stage to develop antimicrobial nanoparticle formulations for applications in chronic wound treatment.

No MeSH data available.


Inhibitory effect of NO/THCPSi NPs (0.1 mg/mL) on bacterialcultures.E. coli (blue bars), S. aureus(yellow bars), and P. aeruginosa (green bars) after 24 hof incubation in TSB medium (37°C, initial bacteria density104 CFU/mL; n = 3;mean ± standard deviation shown).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4109794&req=5

Figure 3: Inhibitory effect of NO/THCPSi NPs (0.1 mg/mL) on bacterialcultures.E. coli (blue bars), S. aureus(yellow bars), and P. aeruginosa (green bars) after 24 hof incubation in TSB medium (37°C, initial bacteria density104 CFU/mL; n = 3;mean ± standard deviation shown).

Mentions: Wound contamination by pathogens such as P. aeruginosa, S.aureus, and E. coli is responsible for a significant morbidityload, particularly in burns and immunocompromised patients [8,31,32]. Initial tests of the antibacterial activity of NO/THCPSi NPs(fabricated by the heating method) were performed against planctonic P.aeruginosa, E. coli, and S. aureus(104 CFU/mL for all) treated with 0.1 mg/mL of NPs for24 h. Compared to the controls (the bacteria cultured without NPs andbacteria treated with glucose/THCPSi NPs), the NO/THCPSi NPs showed significantgrowth inhibition against all three bacteria species tested (seeFigure 3). After the 24-h incubation with0.1 mg/mL of NO/THCPSi NPs, the bacterial counts of P. aeruginosa,S. aureus, and E. coli cultures were reduced approximately1 log in comparison with bacteria cultured in the absence of NPs.


Nitric oxide-releasing porous silicon nanoparticles.

Kafshgari MH, Cavallaro A, Delalat B, Harding FJ, McInnes SJ, Mäkilä E, Salonen J, Vasilev K, Voelcker NH - Nanoscale Res Lett (2014)

Inhibitory effect of NO/THCPSi NPs (0.1 mg/mL) on bacterialcultures.E. coli (blue bars), S. aureus(yellow bars), and P. aeruginosa (green bars) after 24 hof incubation in TSB medium (37°C, initial bacteria density104 CFU/mL; n = 3;mean ± standard deviation shown).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109794&req=5

Figure 3: Inhibitory effect of NO/THCPSi NPs (0.1 mg/mL) on bacterialcultures.E. coli (blue bars), S. aureus(yellow bars), and P. aeruginosa (green bars) after 24 hof incubation in TSB medium (37°C, initial bacteria density104 CFU/mL; n = 3;mean ± standard deviation shown).
Mentions: Wound contamination by pathogens such as P. aeruginosa, S.aureus, and E. coli is responsible for a significant morbidityload, particularly in burns and immunocompromised patients [8,31,32]. Initial tests of the antibacterial activity of NO/THCPSi NPs(fabricated by the heating method) were performed against planctonic P.aeruginosa, E. coli, and S. aureus(104 CFU/mL for all) treated with 0.1 mg/mL of NPs for24 h. Compared to the controls (the bacteria cultured without NPs andbacteria treated with glucose/THCPSi NPs), the NO/THCPSi NPs showed significantgrowth inhibition against all three bacteria species tested (seeFigure 3). After the 24-h incubation with0.1 mg/mL of NO/THCPSi NPs, the bacterial counts of P. aeruginosa,S. aureus, and E. coli cultures were reduced approximately1 log in comparison with bacteria cultured in the absence of NPs.

Bottom Line: Subsequent reduction of nitrite in the presence of d-glucose led to the production of large NO payloads without reducing the biocompatibility of the PSi NPs with mammalian cells.The resulting PSi NPs demonstrated sustained release of NO and showed remarkable antibacterial efficiency and anti-biofilm-forming properties.These results will set the stage to develop antimicrobial nanoparticle formulations for applications in chronic wound treatment.

View Article: PubMed Central - HTML - PubMed

Affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Mawson Institute, University of South Australia, GPO Box 2471 Adelaide, SA 5001, Australia.

ABSTRACT
In this study, the ability of porous silicon nanoparticles (PSi NPs) to entrap and deliver nitric oxide (NO) as an effective antibacterial agent is tested against different Gram-positive and Gram-negative bacteria. NO was entrapped inside PSi NPs functionalized by means of the thermal hydrocarbonization (THC) process. Subsequent reduction of nitrite in the presence of d-glucose led to the production of large NO payloads without reducing the biocompatibility of the PSi NPs with mammalian cells. The resulting PSi NPs demonstrated sustained release of NO and showed remarkable antibacterial efficiency and anti-biofilm-forming properties. These results will set the stage to develop antimicrobial nanoparticle formulations for applications in chronic wound treatment.

No MeSH data available.