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Nitric oxide-releasing porous silicon nanoparticles.

Kafshgari MH, Cavallaro A, Delalat B, Harding FJ, McInnes SJ, Mäkilä E, Salonen J, Vasilev K, Voelcker NH - Nanoscale Res Lett (2014)

Bottom Line: Subsequent reduction of nitrite in the presence of d-glucose led to the production of large NO payloads without reducing the biocompatibility of the PSi NPs with mammalian cells.The resulting PSi NPs demonstrated sustained release of NO and showed remarkable antibacterial efficiency and anti-biofilm-forming properties.These results will set the stage to develop antimicrobial nanoparticle formulations for applications in chronic wound treatment.

View Article: PubMed Central - HTML - PubMed

Affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Mawson Institute, University of South Australia, GPO Box 2471 Adelaide, SA 5001, Australia.

ABSTRACT
In this study, the ability of porous silicon nanoparticles (PSi NPs) to entrap and deliver nitric oxide (NO) as an effective antibacterial agent is tested against different Gram-positive and Gram-negative bacteria. NO was entrapped inside PSi NPs functionalized by means of the thermal hydrocarbonization (THC) process. Subsequent reduction of nitrite in the presence of d-glucose led to the production of large NO payloads without reducing the biocompatibility of the PSi NPs with mammalian cells. The resulting PSi NPs demonstrated sustained release of NO and showed remarkable antibacterial efficiency and anti-biofilm-forming properties. These results will set the stage to develop antimicrobial nanoparticle formulations for applications in chronic wound treatment.

No MeSH data available.


DRIFT absorbance spectra for PSi NPs. (a) THCPSi NPs, (b)glucose/THCPSi NPs, (c) sodium nitrite/THCPSi NPs, and (d)NO/THCPSi NPs.
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Figure 1: DRIFT absorbance spectra for PSi NPs. (a) THCPSi NPs, (b)glucose/THCPSi NPs, (c) sodium nitrite/THCPSi NPs, and (d)NO/THCPSi NPs.

Mentions: DRIFT spectroscopy was used to chemically characterize PSi NPs. In order toscrutinize the nitrite reduction reaction used to prepare the NO/THCPSi NPs,DRIFT spectra of the prepared THCPSi NPs (control a), glucose/THCPSi NPs(control b), sodium nitrite/THCPSi NPs (control c), and NO/THCPSi NPs wereobtained (see Figure 1). The DRIFT spectra obtainedfrom all PSi NPs showed a common set of bands, such as C-H vibration(2,856 cm-1), related to the thermal hydrocarbonization [40]. The NO/THCPSi NPs spectrum presented a N-O stretching vibration(dipole moment 0.4344 Debye) at 1,720 cm-1, indicatingentrapment of NO within the NPs [41]. Moreover, in the spectra of the NO/THCPSi NPs and sodiumnitrite/THCPSi NPs, an intense combination band corresponding toO-N = O around 2,670 cm-1 was observed [42]. The band related to the O-N = O bending vibration(dipole moment 3.8752 Debye) in the NO/THCPSi NPs is likely to be the result ofunreduced sodium nitrite remaining in the NPs. In addition, the presence of theO-H stretching vibrations for NO/THCPSi NPs and glucose/THCPSi NPs indicates thepresence of glucose on the NO/THCPSi NPs. A 35% decrease in nitrite bandintensity compared to sodium nitrite/THCPSi NPs (normalized between spectrabased on C-H vibration at 2,856 cm-1) is evidence of thereduction reaction of nitrite during preparation of NO/THCPSi NPs.


Nitric oxide-releasing porous silicon nanoparticles.

Kafshgari MH, Cavallaro A, Delalat B, Harding FJ, McInnes SJ, Mäkilä E, Salonen J, Vasilev K, Voelcker NH - Nanoscale Res Lett (2014)

DRIFT absorbance spectra for PSi NPs. (a) THCPSi NPs, (b)glucose/THCPSi NPs, (c) sodium nitrite/THCPSi NPs, and (d)NO/THCPSi NPs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109794&req=5

Figure 1: DRIFT absorbance spectra for PSi NPs. (a) THCPSi NPs, (b)glucose/THCPSi NPs, (c) sodium nitrite/THCPSi NPs, and (d)NO/THCPSi NPs.
Mentions: DRIFT spectroscopy was used to chemically characterize PSi NPs. In order toscrutinize the nitrite reduction reaction used to prepare the NO/THCPSi NPs,DRIFT spectra of the prepared THCPSi NPs (control a), glucose/THCPSi NPs(control b), sodium nitrite/THCPSi NPs (control c), and NO/THCPSi NPs wereobtained (see Figure 1). The DRIFT spectra obtainedfrom all PSi NPs showed a common set of bands, such as C-H vibration(2,856 cm-1), related to the thermal hydrocarbonization [40]. The NO/THCPSi NPs spectrum presented a N-O stretching vibration(dipole moment 0.4344 Debye) at 1,720 cm-1, indicatingentrapment of NO within the NPs [41]. Moreover, in the spectra of the NO/THCPSi NPs and sodiumnitrite/THCPSi NPs, an intense combination band corresponding toO-N = O around 2,670 cm-1 was observed [42]. The band related to the O-N = O bending vibration(dipole moment 3.8752 Debye) in the NO/THCPSi NPs is likely to be the result ofunreduced sodium nitrite remaining in the NPs. In addition, the presence of theO-H stretching vibrations for NO/THCPSi NPs and glucose/THCPSi NPs indicates thepresence of glucose on the NO/THCPSi NPs. A 35% decrease in nitrite bandintensity compared to sodium nitrite/THCPSi NPs (normalized between spectrabased on C-H vibration at 2,856 cm-1) is evidence of thereduction reaction of nitrite during preparation of NO/THCPSi NPs.

Bottom Line: Subsequent reduction of nitrite in the presence of d-glucose led to the production of large NO payloads without reducing the biocompatibility of the PSi NPs with mammalian cells.The resulting PSi NPs demonstrated sustained release of NO and showed remarkable antibacterial efficiency and anti-biofilm-forming properties.These results will set the stage to develop antimicrobial nanoparticle formulations for applications in chronic wound treatment.

View Article: PubMed Central - HTML - PubMed

Affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Mawson Institute, University of South Australia, GPO Box 2471 Adelaide, SA 5001, Australia.

ABSTRACT
In this study, the ability of porous silicon nanoparticles (PSi NPs) to entrap and deliver nitric oxide (NO) as an effective antibacterial agent is tested against different Gram-positive and Gram-negative bacteria. NO was entrapped inside PSi NPs functionalized by means of the thermal hydrocarbonization (THC) process. Subsequent reduction of nitrite in the presence of d-glucose led to the production of large NO payloads without reducing the biocompatibility of the PSi NPs with mammalian cells. The resulting PSi NPs demonstrated sustained release of NO and showed remarkable antibacterial efficiency and anti-biofilm-forming properties. These results will set the stage to develop antimicrobial nanoparticle formulations for applications in chronic wound treatment.

No MeSH data available.