Limits...
Compartmental modeling of whole-body vitamin A kinetics in unsupplemented and vitamin A-retinoic acid-supplemented neonatal rats.

Tan L, Wray AE, Green MH, Ross AC - J. Lipid Res. (2014)

Bottom Line: First, compartmental models for retinol kinetics were developed for individual tissues, and then an integrated compartmental model incorporating all tissues was developed for both groups.VARA increased CM retinyl ester uptake by lung, carcass, and intestine; decreased the release into plasma of retinol that had been cleared by liver and lung as CM retinyl esters; stimulated the uptake of retinol from plasma holo-retinol binding protein into carcass; and decreased the retinol turnover out of the liver.Overall, neonatal VA trafficking differed from that previously described for adult animals, with a larger contribution of extrahepatic tissues to CM clearance, especially after VA supplementation, and a significant amount of VA distributed in extrahepatic tissues.

View Article: PubMed Central - PubMed

Affiliation: Graduate Program in Nutrition, The Pennsylvania State University, University Park, PA 16802 Department of Nutritional Sciences, The Pennsylvania State University, University Park, PA 16802.

No MeSH data available.


Related in: MedlinePlus

Mean observed fraction of administered dose in kidney (A), carcass (B), stomach (C), intestine (D), heart (E), and thymus (F) versus time (days) after administration of [3H]retinol in oil or in VARA to neonatal rats. Each point represents the mean of n = 3 pups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4109768&req=5

fig2: Mean observed fraction of administered dose in kidney (A), carcass (B), stomach (C), intestine (D), heart (E), and thymus (F) versus time (days) after administration of [3H]retinol in oil or in VARA to neonatal rats. Each point represents the mean of n = 3 pups.

Mentions: Data on fraction of the oral dose remaining in kidney, carcass, stomach, intestine, thymus, and heart versus time after dose administration are plotted in Fig. 2. The fraction of the dose in kidney (Fig. 2A) in the control neonates was higher than that in the VARA-treated neonates at most time points; however, the pattern of the kinetic response was similar between groups. A rise in radioactivity was observed within 1–11 h after dose administration, and then it decreased until the end of the study. The remaining carcass (Fig. 2B), which was mainly composed of skin, bone, muscle, brain, and adipose tissue, contained the second highest fraction of the ingested dose, after liver. In both groups, radioactivity rose during the first several hours to a peak at 4–6 h where the fraction of dose was ∼10% to 12%. Following a rapid decrease after the peak, there was an increase in the fraction of dose from 1 day to 6 days after dose administration. After day 6, the fraction of dose in the carcass decreased in both groups.


Compartmental modeling of whole-body vitamin A kinetics in unsupplemented and vitamin A-retinoic acid-supplemented neonatal rats.

Tan L, Wray AE, Green MH, Ross AC - J. Lipid Res. (2014)

Mean observed fraction of administered dose in kidney (A), carcass (B), stomach (C), intestine (D), heart (E), and thymus (F) versus time (days) after administration of [3H]retinol in oil or in VARA to neonatal rats. Each point represents the mean of n = 3 pups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109768&req=5

fig2: Mean observed fraction of administered dose in kidney (A), carcass (B), stomach (C), intestine (D), heart (E), and thymus (F) versus time (days) after administration of [3H]retinol in oil or in VARA to neonatal rats. Each point represents the mean of n = 3 pups.
Mentions: Data on fraction of the oral dose remaining in kidney, carcass, stomach, intestine, thymus, and heart versus time after dose administration are plotted in Fig. 2. The fraction of the dose in kidney (Fig. 2A) in the control neonates was higher than that in the VARA-treated neonates at most time points; however, the pattern of the kinetic response was similar between groups. A rise in radioactivity was observed within 1–11 h after dose administration, and then it decreased until the end of the study. The remaining carcass (Fig. 2B), which was mainly composed of skin, bone, muscle, brain, and adipose tissue, contained the second highest fraction of the ingested dose, after liver. In both groups, radioactivity rose during the first several hours to a peak at 4–6 h where the fraction of dose was ∼10% to 12%. Following a rapid decrease after the peak, there was an increase in the fraction of dose from 1 day to 6 days after dose administration. After day 6, the fraction of dose in the carcass decreased in both groups.

Bottom Line: First, compartmental models for retinol kinetics were developed for individual tissues, and then an integrated compartmental model incorporating all tissues was developed for both groups.VARA increased CM retinyl ester uptake by lung, carcass, and intestine; decreased the release into plasma of retinol that had been cleared by liver and lung as CM retinyl esters; stimulated the uptake of retinol from plasma holo-retinol binding protein into carcass; and decreased the retinol turnover out of the liver.Overall, neonatal VA trafficking differed from that previously described for adult animals, with a larger contribution of extrahepatic tissues to CM clearance, especially after VA supplementation, and a significant amount of VA distributed in extrahepatic tissues.

View Article: PubMed Central - PubMed

Affiliation: Graduate Program in Nutrition, The Pennsylvania State University, University Park, PA 16802 Department of Nutritional Sciences, The Pennsylvania State University, University Park, PA 16802.

No MeSH data available.


Related in: MedlinePlus