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Genetic evaluation of mesenchymal stem cells by G-banded karyotyping in a Cell Technology Center.

Borgonovo T, Vaz IM, Senegaglia AC, Rebelatto CL, Brofman PR - Rev Bras Hematol Hemoter (2014)

Bottom Line: To present the initial results of first three years of implementation of a genetic evaluation test for bone marrow-derived mesenchymal stem cells in a Cell Technology Center.All the samples analyzed, before and after cell expansion, had normal karyotypes, showing no clonal chromosomal changes.Signs of chromosomal instability were observed in 11 out of 21 patients (52%).

View Article: PubMed Central - PubMed

Affiliation: Pontifícia Universidade Católica do Paraná (PUC-PR), Curitiba, PR, Brazil. Electronic address: tamaraborgonovo@yahoo.com.br.

No MeSH data available.


Related in: MedlinePlus

Case 20; bone marrow. Partial karyogram showing chromatid breaks in the long arm of chromosome 8.
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fig0010: Case 20; bone marrow. Partial karyogram showing chromatid breaks in the long arm of chromosome 8.

Mentions: Signs of chromosomal instability were observed in 11 (52%) out of 21 patients. From a total of 910 analyzed metaphases, five chromatid gaps, six chromatid breaks (Figure 2) and 14 tetraploid cells were detected giving a total of 25 metaphases with chromosome damage (2.75%).


Genetic evaluation of mesenchymal stem cells by G-banded karyotyping in a Cell Technology Center.

Borgonovo T, Vaz IM, Senegaglia AC, Rebelatto CL, Brofman PR - Rev Bras Hematol Hemoter (2014)

Case 20; bone marrow. Partial karyogram showing chromatid breaks in the long arm of chromosome 8.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109748&req=5

fig0010: Case 20; bone marrow. Partial karyogram showing chromatid breaks in the long arm of chromosome 8.
Mentions: Signs of chromosomal instability were observed in 11 (52%) out of 21 patients. From a total of 910 analyzed metaphases, five chromatid gaps, six chromatid breaks (Figure 2) and 14 tetraploid cells were detected giving a total of 25 metaphases with chromosome damage (2.75%).

Bottom Line: To present the initial results of first three years of implementation of a genetic evaluation test for bone marrow-derived mesenchymal stem cells in a Cell Technology Center.All the samples analyzed, before and after cell expansion, had normal karyotypes, showing no clonal chromosomal changes.Signs of chromosomal instability were observed in 11 out of 21 patients (52%).

View Article: PubMed Central - PubMed

Affiliation: Pontifícia Universidade Católica do Paraná (PUC-PR), Curitiba, PR, Brazil. Electronic address: tamaraborgonovo@yahoo.com.br.

No MeSH data available.


Related in: MedlinePlus