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Murine leukemia virus-derived retroviral vector has differential integration patterns in human cell lines used to produce recombinant factor VIII.

Correa de Freitas MC, Fontes AM, de Castilho Fernandes A, Picanço-Castro V, de Sousa Russo EM, Covas DT - Rev Bras Hematol Hemoter (2014)

Bottom Line: The sequences were compared to genomic databases to characterize respective clones.Twenty percent of integrations occurred at fragile sites in the genome of the HepG2 cell line and 17% in Hek293.The results suggest that the cell type can affect the profile of chromosomal integration of the retroviral vector used; these differences may interfere in the level of expression of recombinant proteins.

View Article: PubMed Central - PubMed

Affiliation: Universidade de São Paulo (USP), Ribeirão Preto, SP, Brazil. Electronic address: marcelafreitas@usp.br.

No MeSH data available.


Related in: MedlinePlus

Integration frequency of pMFG-FVIII-P140K retroviral vector in fragile sites of both HepG2FVIIIdB/P140K and Hek293FVIIIdB/P140K cell lines compared with an expected random integration.
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fig0015: Integration frequency of pMFG-FVIII-P140K retroviral vector in fragile sites of both HepG2FVIIIdB/P140K and Hek293FVIIIdB/P140K cell lines compared with an expected random integration.

Mentions: When retroviral integrations in fragile sites were analyzed, the HepG2/FVIIIdBP140K cells showed 20% of integrations in fragile sites whereas Hek293/FVIIIdBP140K cells showed around 17%. Fragile sites are classified into rare and common according to their frequency and characteristics on chromosomes. Integrations were observed in both groups. As shown in Figure 3, the most affected fragile sites of HepG2 cells were FRA11G (5.35%) and FRA11B (5.35%), whereas the most affected sites for Hek293 were FRA2B (3.77%) and FRA1H (3.77%). When these results are compared with a set of 10,000 random integrations simulated by the Quickmap computer program, there was a difference in the percentage of integration as the integration was random; expected frequencies would be 0.18% of fragile sites for HepG2 and 0.44% for Hek293.


Murine leukemia virus-derived retroviral vector has differential integration patterns in human cell lines used to produce recombinant factor VIII.

Correa de Freitas MC, Fontes AM, de Castilho Fernandes A, Picanço-Castro V, de Sousa Russo EM, Covas DT - Rev Bras Hematol Hemoter (2014)

Integration frequency of pMFG-FVIII-P140K retroviral vector in fragile sites of both HepG2FVIIIdB/P140K and Hek293FVIIIdB/P140K cell lines compared with an expected random integration.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109740&req=5

fig0015: Integration frequency of pMFG-FVIII-P140K retroviral vector in fragile sites of both HepG2FVIIIdB/P140K and Hek293FVIIIdB/P140K cell lines compared with an expected random integration.
Mentions: When retroviral integrations in fragile sites were analyzed, the HepG2/FVIIIdBP140K cells showed 20% of integrations in fragile sites whereas Hek293/FVIIIdBP140K cells showed around 17%. Fragile sites are classified into rare and common according to their frequency and characteristics on chromosomes. Integrations were observed in both groups. As shown in Figure 3, the most affected fragile sites of HepG2 cells were FRA11G (5.35%) and FRA11B (5.35%), whereas the most affected sites for Hek293 were FRA2B (3.77%) and FRA1H (3.77%). When these results are compared with a set of 10,000 random integrations simulated by the Quickmap computer program, there was a difference in the percentage of integration as the integration was random; expected frequencies would be 0.18% of fragile sites for HepG2 and 0.44% for Hek293.

Bottom Line: The sequences were compared to genomic databases to characterize respective clones.Twenty percent of integrations occurred at fragile sites in the genome of the HepG2 cell line and 17% in Hek293.The results suggest that the cell type can affect the profile of chromosomal integration of the retroviral vector used; these differences may interfere in the level of expression of recombinant proteins.

View Article: PubMed Central - PubMed

Affiliation: Universidade de São Paulo (USP), Ribeirão Preto, SP, Brazil. Electronic address: marcelafreitas@usp.br.

No MeSH data available.


Related in: MedlinePlus