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Murine leukemia virus-derived retroviral vector has differential integration patterns in human cell lines used to produce recombinant factor VIII.

Correa de Freitas MC, Fontes AM, de Castilho Fernandes A, Picanço-Castro V, de Sousa Russo EM, Covas DT - Rev Bras Hematol Hemoter (2014)

Bottom Line: The sequences were compared to genomic databases to characterize respective clones.Twenty percent of integrations occurred at fragile sites in the genome of the HepG2 cell line and 17% in Hek293.The results suggest that the cell type can affect the profile of chromosomal integration of the retroviral vector used; these differences may interfere in the level of expression of recombinant proteins.

View Article: PubMed Central - PubMed

Affiliation: Universidade de São Paulo (USP), Ribeirão Preto, SP, Brazil. Electronic address: marcelafreitas@usp.br.

No MeSH data available.


Related in: MedlinePlus

(A) Number of retroviral vector integrations observed in regions near to transcription factor binding sites (TFBS) (p-value = 0.606). (B) Number of retroviral vector integrations observed in regions near to CpG island (p-value = 0.5462).
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fig0010: (A) Number of retroviral vector integrations observed in regions near to transcription factor binding sites (TFBS) (p-value = 0.606). (B) Number of retroviral vector integrations observed in regions near to CpG island (p-value = 0.5462).

Mentions: In this study, a total of 140 TFBS were affected by integrations that occurred in HepG2FVIIIdB/P140K cells. Of these, five integrations occurred in areas within 5 kb, nine integrations within 10 kb and 39 within 30 kb of TFBS. In Hek293FVIIIdB/P140K kidney cells, a total of 117 TFBS were affected, and only two insertions occurred within 5 kb, one integration within 10 kb and nine within 30 kb of TFBS. The integration profiles of TFBS regions showed no statistically significant differences between the two cell lines according to Fisher's exact test (p-value = 0.606) as shown in Figure 2A.


Murine leukemia virus-derived retroviral vector has differential integration patterns in human cell lines used to produce recombinant factor VIII.

Correa de Freitas MC, Fontes AM, de Castilho Fernandes A, Picanço-Castro V, de Sousa Russo EM, Covas DT - Rev Bras Hematol Hemoter (2014)

(A) Number of retroviral vector integrations observed in regions near to transcription factor binding sites (TFBS) (p-value = 0.606). (B) Number of retroviral vector integrations observed in regions near to CpG island (p-value = 0.5462).
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109740&req=5

fig0010: (A) Number of retroviral vector integrations observed in regions near to transcription factor binding sites (TFBS) (p-value = 0.606). (B) Number of retroviral vector integrations observed in regions near to CpG island (p-value = 0.5462).
Mentions: In this study, a total of 140 TFBS were affected by integrations that occurred in HepG2FVIIIdB/P140K cells. Of these, five integrations occurred in areas within 5 kb, nine integrations within 10 kb and 39 within 30 kb of TFBS. In Hek293FVIIIdB/P140K kidney cells, a total of 117 TFBS were affected, and only two insertions occurred within 5 kb, one integration within 10 kb and nine within 30 kb of TFBS. The integration profiles of TFBS regions showed no statistically significant differences between the two cell lines according to Fisher's exact test (p-value = 0.606) as shown in Figure 2A.

Bottom Line: The sequences were compared to genomic databases to characterize respective clones.Twenty percent of integrations occurred at fragile sites in the genome of the HepG2 cell line and 17% in Hek293.The results suggest that the cell type can affect the profile of chromosomal integration of the retroviral vector used; these differences may interfere in the level of expression of recombinant proteins.

View Article: PubMed Central - PubMed

Affiliation: Universidade de São Paulo (USP), Ribeirão Preto, SP, Brazil. Electronic address: marcelafreitas@usp.br.

No MeSH data available.


Related in: MedlinePlus