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Murine leukemia virus-derived retroviral vector has differential integration patterns in human cell lines used to produce recombinant factor VIII.

Correa de Freitas MC, Fontes AM, de Castilho Fernandes A, Picanço-Castro V, de Sousa Russo EM, Covas DT - Rev Bras Hematol Hemoter (2014)

Bottom Line: The sequences were compared to genomic databases to characterize respective clones.Twenty percent of integrations occurred at fragile sites in the genome of the HepG2 cell line and 17% in Hek293.The results suggest that the cell type can affect the profile of chromosomal integration of the retroviral vector used; these differences may interfere in the level of expression of recombinant proteins.

View Article: PubMed Central - PubMed

Affiliation: Universidade de São Paulo (USP), Ribeirão Preto, SP, Brazil. Electronic address: marcelafreitas@usp.br.

No MeSH data available.


Related in: MedlinePlus

(A) Integration pattern of retroviral vector pMFG-FVIII-P140K in the 23 chromosomes of two cell lines producing recombinant factor VIII. (B) RISC score – number of chromosomal integrations observed versus number of expected integrations of the PMFG-FVIII-P140K vector in the HepG2FVIIIdB/P140K and Hek293FVIIIdB/P140K cell lines (cut-off = 3).
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fig0005: (A) Integration pattern of retroviral vector pMFG-FVIII-P140K in the 23 chromosomes of two cell lines producing recombinant factor VIII. (B) RISC score – number of chromosomal integrations observed versus number of expected integrations of the PMFG-FVIII-P140K vector in the HepG2FVIIIdB/P140K and Hek293FVIIIdB/P140K cell lines (cut-off = 3).

Mentions: HepG2/FVIIIdBP140K clone sequences showed that integrations occurred in 18 of 23 chromosomes with preference for specific chromosomes. For these cells, 19% of the integrations occurred within chromosome 19, followed by 16% within chromosome 17 and 9.6% within chromosome 11. For the Hek293FVIIIdB/P140K cells, insertions affected 20 of the 23 chromosomes, with high frequencies of integrations occurring within chromosomes 9 (14%) and X (9%) (Figure 1A).


Murine leukemia virus-derived retroviral vector has differential integration patterns in human cell lines used to produce recombinant factor VIII.

Correa de Freitas MC, Fontes AM, de Castilho Fernandes A, Picanço-Castro V, de Sousa Russo EM, Covas DT - Rev Bras Hematol Hemoter (2014)

(A) Integration pattern of retroviral vector pMFG-FVIII-P140K in the 23 chromosomes of two cell lines producing recombinant factor VIII. (B) RISC score – number of chromosomal integrations observed versus number of expected integrations of the PMFG-FVIII-P140K vector in the HepG2FVIIIdB/P140K and Hek293FVIIIdB/P140K cell lines (cut-off = 3).
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109740&req=5

fig0005: (A) Integration pattern of retroviral vector pMFG-FVIII-P140K in the 23 chromosomes of two cell lines producing recombinant factor VIII. (B) RISC score – number of chromosomal integrations observed versus number of expected integrations of the PMFG-FVIII-P140K vector in the HepG2FVIIIdB/P140K and Hek293FVIIIdB/P140K cell lines (cut-off = 3).
Mentions: HepG2/FVIIIdBP140K clone sequences showed that integrations occurred in 18 of 23 chromosomes with preference for specific chromosomes. For these cells, 19% of the integrations occurred within chromosome 19, followed by 16% within chromosome 17 and 9.6% within chromosome 11. For the Hek293FVIIIdB/P140K cells, insertions affected 20 of the 23 chromosomes, with high frequencies of integrations occurring within chromosomes 9 (14%) and X (9%) (Figure 1A).

Bottom Line: The sequences were compared to genomic databases to characterize respective clones.Twenty percent of integrations occurred at fragile sites in the genome of the HepG2 cell line and 17% in Hek293.The results suggest that the cell type can affect the profile of chromosomal integration of the retroviral vector used; these differences may interfere in the level of expression of recombinant proteins.

View Article: PubMed Central - PubMed

Affiliation: Universidade de São Paulo (USP), Ribeirão Preto, SP, Brazil. Electronic address: marcelafreitas@usp.br.

No MeSH data available.


Related in: MedlinePlus