Limits...
De novo alpha 2 hemoglobin gene (HBA2) mutation in a child with hemoglobin M Iwate and symptomatic methemoglobinemia since birth.

Viana MB, Belisário AR - Rev Bras Hematol Hemoter (2014)

Bottom Line: The newborn child was treated with methylene blue in an intensive care unit fearing that he had a defective reductase system and exposure to oxidant drugs or toxins.Newborn hemoglobin screening with high performance liquid chromatography was abnormal on the 10th and 45th days but no conclusive diagnosis was reached.Except for cosmetic consequences, the clinical course of patients with hemoglobin M Iwate is unremarkable.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Pediatria da Faculdade de Medicina, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil. Electronic address: vianamb@gmail.com.

No MeSH data available.


Related in: MedlinePlus

Hemoglobin electrophoresis in alkaline (A) and acid gel (B), and by isoelectric focusing (C and D). Hb M Iwate is anodal to Hb A in alkaline gel and undistinguishable from Hb A in acid gel. Its color is brown in contrast to the vivid red of Hb A before trichloroacetic acid is applied to the isoelectric focusing gel (C). It runs between Hb F and Hb S (closer to Hb F) after the gel has been fixed and dried (D).
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4109735&req=5

fig0010: Hemoglobin electrophoresis in alkaline (A) and acid gel (B), and by isoelectric focusing (C and D). Hb M Iwate is anodal to Hb A in alkaline gel and undistinguishable from Hb A in acid gel. Its color is brown in contrast to the vivid red of Hb A before trichloroacetic acid is applied to the isoelectric focusing gel (C). It runs between Hb F and Hb S (closer to Hb F) after the gel has been fixed and dried (D).

Mentions: Figure 2 depicts alkaline and acid gel electrophoresis and IEF from the sample drawn for this report. Note that Hb M Iwate is clearly separated from Hb F and Hb A in both the alkaline gel procedure and IEF, but indistinguishable from Hb A in acid medium. The estimated relative concentration of Hb M Iwate read in the alkaline gel was 28%. Hb A2 concentration was 3% and Hb F 1.9%. In IEF, the color of Hb M Iwate is brown before trichloroacetic acid is applied to the IEF gel, in contrast to the vivid red color of Hb A (Figure 2C).


De novo alpha 2 hemoglobin gene (HBA2) mutation in a child with hemoglobin M Iwate and symptomatic methemoglobinemia since birth.

Viana MB, Belisário AR - Rev Bras Hematol Hemoter (2014)

Hemoglobin electrophoresis in alkaline (A) and acid gel (B), and by isoelectric focusing (C and D). Hb M Iwate is anodal to Hb A in alkaline gel and undistinguishable from Hb A in acid gel. Its color is brown in contrast to the vivid red of Hb A before trichloroacetic acid is applied to the isoelectric focusing gel (C). It runs between Hb F and Hb S (closer to Hb F) after the gel has been fixed and dried (D).
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109735&req=5

fig0010: Hemoglobin electrophoresis in alkaline (A) and acid gel (B), and by isoelectric focusing (C and D). Hb M Iwate is anodal to Hb A in alkaline gel and undistinguishable from Hb A in acid gel. Its color is brown in contrast to the vivid red of Hb A before trichloroacetic acid is applied to the isoelectric focusing gel (C). It runs between Hb F and Hb S (closer to Hb F) after the gel has been fixed and dried (D).
Mentions: Figure 2 depicts alkaline and acid gel electrophoresis and IEF from the sample drawn for this report. Note that Hb M Iwate is clearly separated from Hb F and Hb A in both the alkaline gel procedure and IEF, but indistinguishable from Hb A in acid medium. The estimated relative concentration of Hb M Iwate read in the alkaline gel was 28%. Hb A2 concentration was 3% and Hb F 1.9%. In IEF, the color of Hb M Iwate is brown before trichloroacetic acid is applied to the IEF gel, in contrast to the vivid red color of Hb A (Figure 2C).

Bottom Line: The newborn child was treated with methylene blue in an intensive care unit fearing that he had a defective reductase system and exposure to oxidant drugs or toxins.Newborn hemoglobin screening with high performance liquid chromatography was abnormal on the 10th and 45th days but no conclusive diagnosis was reached.Except for cosmetic consequences, the clinical course of patients with hemoglobin M Iwate is unremarkable.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Pediatria da Faculdade de Medicina, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil. Electronic address: vianamb@gmail.com.

No MeSH data available.


Related in: MedlinePlus