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In vitro and in vivo characterization of the new analgesic combination Beta-caryophyllene and docosahexaenoic Acid.

Fiorenzani P, Lamponi S, Magnani A, Ceccarelli I, Aloisi AM - Evid Based Complement Alternat Med (2014)

Bottom Line: BCP changed fibroblast and astrocyte survival in a dose-dependent manner and the effect was counteracted by DHA coadministration.In the in vivo tests, pain responses were significantly decreased in the BCP and BCP+DHA groups with respect to OIL after 1 and 2 weeks of treatment.In conclusion, BCP alone or at lower concentration in combination with DHA was efficacious in modulating pain, showing a clear analgesic activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, Surgery and Neuroscience, University of Siena, Via Aldo Moro, 53100 Siena, Italy.

ABSTRACT
Beta-caryophyllene (BCP) and docosahexaenoic acid (DHA) are components of several plants with documented anti-inflammatory and analgesic effects in animal pain models. In the present study, in vitro and in vivo tests were carried out to evaluate their effects, alone or in combination, during long-lasting administration in a model of persistent pain. IR spectra of the two compounds were obtained to determine their chemical stability and then in vitro toxicity was evaluated in fibroblasts and astrocytes. In the in vivo tests, the analgesic effects of BCP and BCP+DHA were determined in male rats subjected to a model of persistent recurrent pain (three repetitions of the formalin test once a week) to mimic recurrent pain. Both substances were administered per os in almond oil for 2 weeks. Gonadal hormones were determined at the end of the tests to evaluate treatment-induced effects on their levels. BCP changed fibroblast and astrocyte survival in a dose-dependent manner and the effect was counteracted by DHA coadministration. In the in vivo tests, pain responses were significantly decreased in the BCP and BCP+DHA groups with respect to OIL after 1 and 2 weeks of treatment. Estradiol and testosterone levels were increased only in the BCP group. In conclusion, BCP alone or at lower concentration in combination with DHA was efficacious in modulating pain, showing a clear analgesic activity.

No MeSH data available.


Related in: MedlinePlus

Formalin test: time course of formalin-induced flexing (AD), licking (BE), and jerking (CF) of the injected paw during FT2 and FT3 in the OIL (■), BCP (□), and BCP+DHA (▼) groups. ANOVA showed that BCP and BCP+DHA administration significantly reduced the formalin-induced responses, in particular, during FT2. Values are means ± SEM. See Section 3 for details. **P < 0.001 versus BCP, *P < 0.05 versus BCP, ##P < 0.001 versus BCP+DHA, and #P < 0.05 versus BCP+DHA.
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fig7: Formalin test: time course of formalin-induced flexing (AD), licking (BE), and jerking (CF) of the injected paw during FT2 and FT3 in the OIL (■), BCP (□), and BCP+DHA (▼) groups. ANOVA showed that BCP and BCP+DHA administration significantly reduced the formalin-induced responses, in particular, during FT2. Values are means ± SEM. See Section 3 for details. **P < 0.001 versus BCP, *P < 0.05 versus BCP, ##P < 0.001 versus BCP+DHA, and #P < 0.05 versus BCP+DHA.

Mentions: Flexing. One-way ANOVA applied to flexing duration revealed a significant effect of Treatment (F2,220 = 18.26; P < 0.001) due to the significantly lower levels in the BCP and BCP+DHA groups (P < 0.0001 for both) with respect to the OIL group. In particular, the interaction Treatment × Time (F22,220 = 3.8; P < 0.001) showed that the reduction of the formalin-induced flexing of the paw was due to the lower levels from 30 to 45 min of FT2 with respect to OIL-treated animals (P < 0.01 for BCP and P < 0.05 for BCP+DHA) (Figure 7(a)).


In vitro and in vivo characterization of the new analgesic combination Beta-caryophyllene and docosahexaenoic Acid.

Fiorenzani P, Lamponi S, Magnani A, Ceccarelli I, Aloisi AM - Evid Based Complement Alternat Med (2014)

Formalin test: time course of formalin-induced flexing (AD), licking (BE), and jerking (CF) of the injected paw during FT2 and FT3 in the OIL (■), BCP (□), and BCP+DHA (▼) groups. ANOVA showed that BCP and BCP+DHA administration significantly reduced the formalin-induced responses, in particular, during FT2. Values are means ± SEM. See Section 3 for details. **P < 0.001 versus BCP, *P < 0.05 versus BCP, ##P < 0.001 versus BCP+DHA, and #P < 0.05 versus BCP+DHA.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4109702&req=5

fig7: Formalin test: time course of formalin-induced flexing (AD), licking (BE), and jerking (CF) of the injected paw during FT2 and FT3 in the OIL (■), BCP (□), and BCP+DHA (▼) groups. ANOVA showed that BCP and BCP+DHA administration significantly reduced the formalin-induced responses, in particular, during FT2. Values are means ± SEM. See Section 3 for details. **P < 0.001 versus BCP, *P < 0.05 versus BCP, ##P < 0.001 versus BCP+DHA, and #P < 0.05 versus BCP+DHA.
Mentions: Flexing. One-way ANOVA applied to flexing duration revealed a significant effect of Treatment (F2,220 = 18.26; P < 0.001) due to the significantly lower levels in the BCP and BCP+DHA groups (P < 0.0001 for both) with respect to the OIL group. In particular, the interaction Treatment × Time (F22,220 = 3.8; P < 0.001) showed that the reduction of the formalin-induced flexing of the paw was due to the lower levels from 30 to 45 min of FT2 with respect to OIL-treated animals (P < 0.01 for BCP and P < 0.05 for BCP+DHA) (Figure 7(a)).

Bottom Line: BCP changed fibroblast and astrocyte survival in a dose-dependent manner and the effect was counteracted by DHA coadministration.In the in vivo tests, pain responses were significantly decreased in the BCP and BCP+DHA groups with respect to OIL after 1 and 2 weeks of treatment.In conclusion, BCP alone or at lower concentration in combination with DHA was efficacious in modulating pain, showing a clear analgesic activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, Surgery and Neuroscience, University of Siena, Via Aldo Moro, 53100 Siena, Italy.

ABSTRACT
Beta-caryophyllene (BCP) and docosahexaenoic acid (DHA) are components of several plants with documented anti-inflammatory and analgesic effects in animal pain models. In the present study, in vitro and in vivo tests were carried out to evaluate their effects, alone or in combination, during long-lasting administration in a model of persistent pain. IR spectra of the two compounds were obtained to determine their chemical stability and then in vitro toxicity was evaluated in fibroblasts and astrocytes. In the in vivo tests, the analgesic effects of BCP and BCP+DHA were determined in male rats subjected to a model of persistent recurrent pain (three repetitions of the formalin test once a week) to mimic recurrent pain. Both substances were administered per os in almond oil for 2 weeks. Gonadal hormones were determined at the end of the tests to evaluate treatment-induced effects on their levels. BCP changed fibroblast and astrocyte survival in a dose-dependent manner and the effect was counteracted by DHA coadministration. In the in vivo tests, pain responses were significantly decreased in the BCP and BCP+DHA groups with respect to OIL after 1 and 2 weeks of treatment. Estradiol and testosterone levels were increased only in the BCP group. In conclusion, BCP alone or at lower concentration in combination with DHA was efficacious in modulating pain, showing a clear analgesic activity.

No MeSH data available.


Related in: MedlinePlus