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Xiao Yao San Improves Depressive-Like Behavior in Rats through Modulation of β-Arrestin 2-Mediated Pathways in Hippocampus.

Zhu X, Xia O, Han W, Shao M, Jing L, Fan Q, Liu Y, Diao J, Lv Z, Sun X - Evid Based Complement Alternat Med (2014)

Bottom Line: XYS was effective in ameliorating the depressive-like behavior and downregulating UCN2 and CORT.XYS decreased the expression of serine/threonine-protein phosphatase 2A subunit B and increased the expression of β-arrestin 2.In conclusion, XYS improves social isolation and CUMS-induced depressive-like behavior and ameliorates HPA hyperactivation through the downregulation of corticotrophin releasing hormone (CRH) receptor 2.

View Article: PubMed Central - PubMed

Affiliation: Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, No. 1838, Guangzhou 510515, China ; The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China.

ABSTRACT
Xiao Yao San (XYS) is a classical Chinese medicine formula that has been widely used to treat mood disorders for hundreds of years. To confirm the effect of XYS and better understand its underlying mechanism, high-performance liquid chromatography-mass spectrometry analysis-based quality control of XYS extracts and proteomics-based identification of differential proteins in the hippocampus were adopted in social isolation and chronic unpredictable mild stress- (CUMS-) treated rats. The depressive-like behavior of rats induced by CUMS resembled the manifestation of human depression. The upregulated corticosterone (CORT) and urocortin 2 (UCN2) levels demonstrated the existence of hypothalamic-pituitary-adrenal (HPA) axis hyperactivity. XYS was effective in ameliorating the depressive-like behavior and downregulating UCN2 and CORT. XYS decreased the expression of serine/threonine-protein phosphatase 2A subunit B and increased the expression of β-arrestin 2. The expressions of brain-derived neurotrophic factor (BDNF), tyrosine receptor kinase B (TrkB), and mammalian target of rapamycin (mTOR) were also elevated by XYS. In conclusion, XYS improves social isolation and CUMS-induced depressive-like behavior and ameliorates HPA hyperactivation through the downregulation of corticotrophin releasing hormone (CRH) receptor 2. The upregulation of BDNF/TrkB and the phosphorylation of mTOR require β-arrestin 2 as a scaffold to regulate stress signaling.

No MeSH data available.


Related in: MedlinePlus

Representative Western blot analysis (a) and immunohistochemical staining (b) of BDNF, TrkB, p-ERK, ERK, and β-arrestin 2 in the hippocampus. BDNF: brain-derived neurotrophic factor; TrkB: tyrosine kinase receptor B; ERK: extracellular signal-regulated kinase. Refer to Table 2 for the semiquantitative analysis of the above images.
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fig7: Representative Western blot analysis (a) and immunohistochemical staining (b) of BDNF, TrkB, p-ERK, ERK, and β-arrestin 2 in the hippocampus. BDNF: brain-derived neurotrophic factor; TrkB: tyrosine kinase receptor B; ERK: extracellular signal-regulated kinase. Refer to Table 2 for the semiquantitative analysis of the above images.

Mentions: The decreased expression of β-arrestin 2, BDNF, TrkB and the phosphorylation of ERK was observed in the model group unlike the control group. XYS increased the expression of β-arrestin 2, BDNF, and TrkB and the phosphorylation of ERK. The expression patterns of BDNF, TrkB, and β-arrestin 2 and the phosphorylation of ERK were further confirmed by immunohistochemistry (Figure 7).


Xiao Yao San Improves Depressive-Like Behavior in Rats through Modulation of β-Arrestin 2-Mediated Pathways in Hippocampus.

Zhu X, Xia O, Han W, Shao M, Jing L, Fan Q, Liu Y, Diao J, Lv Z, Sun X - Evid Based Complement Alternat Med (2014)

Representative Western blot analysis (a) and immunohistochemical staining (b) of BDNF, TrkB, p-ERK, ERK, and β-arrestin 2 in the hippocampus. BDNF: brain-derived neurotrophic factor; TrkB: tyrosine kinase receptor B; ERK: extracellular signal-regulated kinase. Refer to Table 2 for the semiquantitative analysis of the above images.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4109698&req=5

fig7: Representative Western blot analysis (a) and immunohistochemical staining (b) of BDNF, TrkB, p-ERK, ERK, and β-arrestin 2 in the hippocampus. BDNF: brain-derived neurotrophic factor; TrkB: tyrosine kinase receptor B; ERK: extracellular signal-regulated kinase. Refer to Table 2 for the semiquantitative analysis of the above images.
Mentions: The decreased expression of β-arrestin 2, BDNF, TrkB and the phosphorylation of ERK was observed in the model group unlike the control group. XYS increased the expression of β-arrestin 2, BDNF, and TrkB and the phosphorylation of ERK. The expression patterns of BDNF, TrkB, and β-arrestin 2 and the phosphorylation of ERK were further confirmed by immunohistochemistry (Figure 7).

Bottom Line: XYS was effective in ameliorating the depressive-like behavior and downregulating UCN2 and CORT.XYS decreased the expression of serine/threonine-protein phosphatase 2A subunit B and increased the expression of β-arrestin 2.In conclusion, XYS improves social isolation and CUMS-induced depressive-like behavior and ameliorates HPA hyperactivation through the downregulation of corticotrophin releasing hormone (CRH) receptor 2.

View Article: PubMed Central - PubMed

Affiliation: Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, No. 1838, Guangzhou 510515, China ; The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China.

ABSTRACT
Xiao Yao San (XYS) is a classical Chinese medicine formula that has been widely used to treat mood disorders for hundreds of years. To confirm the effect of XYS and better understand its underlying mechanism, high-performance liquid chromatography-mass spectrometry analysis-based quality control of XYS extracts and proteomics-based identification of differential proteins in the hippocampus were adopted in social isolation and chronic unpredictable mild stress- (CUMS-) treated rats. The depressive-like behavior of rats induced by CUMS resembled the manifestation of human depression. The upregulated corticosterone (CORT) and urocortin 2 (UCN2) levels demonstrated the existence of hypothalamic-pituitary-adrenal (HPA) axis hyperactivity. XYS was effective in ameliorating the depressive-like behavior and downregulating UCN2 and CORT. XYS decreased the expression of serine/threonine-protein phosphatase 2A subunit B and increased the expression of β-arrestin 2. The expressions of brain-derived neurotrophic factor (BDNF), tyrosine receptor kinase B (TrkB), and mammalian target of rapamycin (mTOR) were also elevated by XYS. In conclusion, XYS improves social isolation and CUMS-induced depressive-like behavior and ameliorates HPA hyperactivation through the downregulation of corticotrophin releasing hormone (CRH) receptor 2. The upregulation of BDNF/TrkB and the phosphorylation of mTOR require β-arrestin 2 as a scaffold to regulate stress signaling.

No MeSH data available.


Related in: MedlinePlus