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Xiao Yao San Improves Depressive-Like Behavior in Rats through Modulation of β-Arrestin 2-Mediated Pathways in Hippocampus.

Zhu X, Xia O, Han W, Shao M, Jing L, Fan Q, Liu Y, Diao J, Lv Z, Sun X - Evid Based Complement Alternat Med (2014)

Bottom Line: XYS was effective in ameliorating the depressive-like behavior and downregulating UCN2 and CORT.XYS decreased the expression of serine/threonine-protein phosphatase 2A subunit B and increased the expression of β-arrestin 2.In conclusion, XYS improves social isolation and CUMS-induced depressive-like behavior and ameliorates HPA hyperactivation through the downregulation of corticotrophin releasing hormone (CRH) receptor 2.

View Article: PubMed Central - PubMed

Affiliation: Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, No. 1838, Guangzhou 510515, China ; The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China.

ABSTRACT
Xiao Yao San (XYS) is a classical Chinese medicine formula that has been widely used to treat mood disorders for hundreds of years. To confirm the effect of XYS and better understand its underlying mechanism, high-performance liquid chromatography-mass spectrometry analysis-based quality control of XYS extracts and proteomics-based identification of differential proteins in the hippocampus were adopted in social isolation and chronic unpredictable mild stress- (CUMS-) treated rats. The depressive-like behavior of rats induced by CUMS resembled the manifestation of human depression. The upregulated corticosterone (CORT) and urocortin 2 (UCN2) levels demonstrated the existence of hypothalamic-pituitary-adrenal (HPA) axis hyperactivity. XYS was effective in ameliorating the depressive-like behavior and downregulating UCN2 and CORT. XYS decreased the expression of serine/threonine-protein phosphatase 2A subunit B and increased the expression of β-arrestin 2. The expressions of brain-derived neurotrophic factor (BDNF), tyrosine receptor kinase B (TrkB), and mammalian target of rapamycin (mTOR) were also elevated by XYS. In conclusion, XYS improves social isolation and CUMS-induced depressive-like behavior and ameliorates HPA hyperactivation through the downregulation of corticotrophin releasing hormone (CRH) receptor 2. The upregulation of BDNF/TrkB and the phosphorylation of mTOR require β-arrestin 2 as a scaffold to regulate stress signaling.

No MeSH data available.


Related in: MedlinePlus

Effects of XYS on body weight and behavior of rats treated with social isolation and CUMS. Body weight was measured once a week (a). A battery of behavioral tests was initiated 21 d after modeling, and the following parameters were measured: crossing trajectories (b), crossing numbers (c), rearing numbers (d), sucrose preference (e), and food consumption (f). Data are expressed as mean ± SD, n = 10 per group. △P < 0.05, ▲P < 0.01 versus control, *P < 0.05, #P < 0.01 versus model.
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fig1: Effects of XYS on body weight and behavior of rats treated with social isolation and CUMS. Body weight was measured once a week (a). A battery of behavioral tests was initiated 21 d after modeling, and the following parameters were measured: crossing trajectories (b), crossing numbers (c), rearing numbers (d), sucrose preference (e), and food consumption (f). Data are expressed as mean ± SD, n = 10 per group. △P < 0.05, ▲P < 0.01 versus control, *P < 0.05, #P < 0.01 versus model.

Mentions: Social isolation and CUMS treatment decreased the body weight of rats at 14 d and 21 d (P < 0.01). Moreover, XYS and fluoxetine significantly improved the body weight loss at 14 d and 21 d (P < 0.01) (Figure 1(a)).


Xiao Yao San Improves Depressive-Like Behavior in Rats through Modulation of β-Arrestin 2-Mediated Pathways in Hippocampus.

Zhu X, Xia O, Han W, Shao M, Jing L, Fan Q, Liu Y, Diao J, Lv Z, Sun X - Evid Based Complement Alternat Med (2014)

Effects of XYS on body weight and behavior of rats treated with social isolation and CUMS. Body weight was measured once a week (a). A battery of behavioral tests was initiated 21 d after modeling, and the following parameters were measured: crossing trajectories (b), crossing numbers (c), rearing numbers (d), sucrose preference (e), and food consumption (f). Data are expressed as mean ± SD, n = 10 per group. △P < 0.05, ▲P < 0.01 versus control, *P < 0.05, #P < 0.01 versus model.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4109698&req=5

fig1: Effects of XYS on body weight and behavior of rats treated with social isolation and CUMS. Body weight was measured once a week (a). A battery of behavioral tests was initiated 21 d after modeling, and the following parameters were measured: crossing trajectories (b), crossing numbers (c), rearing numbers (d), sucrose preference (e), and food consumption (f). Data are expressed as mean ± SD, n = 10 per group. △P < 0.05, ▲P < 0.01 versus control, *P < 0.05, #P < 0.01 versus model.
Mentions: Social isolation and CUMS treatment decreased the body weight of rats at 14 d and 21 d (P < 0.01). Moreover, XYS and fluoxetine significantly improved the body weight loss at 14 d and 21 d (P < 0.01) (Figure 1(a)).

Bottom Line: XYS was effective in ameliorating the depressive-like behavior and downregulating UCN2 and CORT.XYS decreased the expression of serine/threonine-protein phosphatase 2A subunit B and increased the expression of β-arrestin 2.In conclusion, XYS improves social isolation and CUMS-induced depressive-like behavior and ameliorates HPA hyperactivation through the downregulation of corticotrophin releasing hormone (CRH) receptor 2.

View Article: PubMed Central - PubMed

Affiliation: Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, No. 1838, Guangzhou 510515, China ; The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China.

ABSTRACT
Xiao Yao San (XYS) is a classical Chinese medicine formula that has been widely used to treat mood disorders for hundreds of years. To confirm the effect of XYS and better understand its underlying mechanism, high-performance liquid chromatography-mass spectrometry analysis-based quality control of XYS extracts and proteomics-based identification of differential proteins in the hippocampus were adopted in social isolation and chronic unpredictable mild stress- (CUMS-) treated rats. The depressive-like behavior of rats induced by CUMS resembled the manifestation of human depression. The upregulated corticosterone (CORT) and urocortin 2 (UCN2) levels demonstrated the existence of hypothalamic-pituitary-adrenal (HPA) axis hyperactivity. XYS was effective in ameliorating the depressive-like behavior and downregulating UCN2 and CORT. XYS decreased the expression of serine/threonine-protein phosphatase 2A subunit B and increased the expression of β-arrestin 2. The expressions of brain-derived neurotrophic factor (BDNF), tyrosine receptor kinase B (TrkB), and mammalian target of rapamycin (mTOR) were also elevated by XYS. In conclusion, XYS improves social isolation and CUMS-induced depressive-like behavior and ameliorates HPA hyperactivation through the downregulation of corticotrophin releasing hormone (CRH) receptor 2. The upregulation of BDNF/TrkB and the phosphorylation of mTOR require β-arrestin 2 as a scaffold to regulate stress signaling.

No MeSH data available.


Related in: MedlinePlus