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Development and characterization of novel porous 3D alginate-cockle shell powder nanobiocomposite bone scaffold.

Bharatham BH, Abu Bakar MZ, Perimal EK, Yusof LM, Hamid M - Biomed Res Int (2014)

Bottom Line: The scaffold was developed in varying composition mixture of Alg-nCP and characterized using various evaluation techniques as well as preliminary in vitro studies on MG63 human osteoblast cells.Morphological observations using SEM revealed variations in structures with the use of different Alg-nCP composition ratios.All the developed scaffolds showed a porous structure with pore sizes ideal for facilitating new bone growth; however, not all combination mixtures showed subsequent favorable characteristics to be used for biological applications.

View Article: PubMed Central - PubMed

Affiliation: Biomedical Sciences Programme, School of Diagnostic and Applied Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia ; Department of Veterinary Preclinical Sciences, Faculty of Veterinary Medicine, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor Darul Ehsan, Malaysia.

ABSTRACT
A novel porous three-dimensional bone scaffold was developed using a natural polymer (alginate/Alg) in combination with a naturally obtained biomineral (nano cockle shell powder/nCP) through lyophilization techniques. The scaffold was developed in varying composition mixture of Alg-nCP and characterized using various evaluation techniques as well as preliminary in vitro studies on MG63 human osteoblast cells. Morphological observations using SEM revealed variations in structures with the use of different Alg-nCP composition ratios. All the developed scaffolds showed a porous structure with pore sizes ideal for facilitating new bone growth; however, not all combination mixtures showed subsequent favorable characteristics to be used for biological applications. Scaffolds produced using the combination mixture of 40% Alg and 60% nCP produced significantly promising results in terms of mechanical strength, degradation rate, and increased cell proliferation rates making it potentially the optimum composition mixture of Alg-nCP with future application prospects.

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Related in: MedlinePlus

Fluorescence stained cells grown in the medium containing leachable of scaffold 1 (a), scaffold 2 (b), scaffold 3 (c), scaffold 4 (d), scaffold 5 (e), and normal culture medium (f) taken at ×10 magnification.
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fig8: Fluorescence stained cells grown in the medium containing leachable of scaffold 1 (a), scaffold 2 (b), scaffold 3 (c), scaffold 4 (d), scaffold 5 (e), and normal culture medium (f) taken at ×10 magnification.

Mentions: The live/dead cell staining procedure was carried out to supplement the results from the MTT assay in which the identifications of viable cells are observed to produce a green fluorescence when the stains are incorporated with the cell's DNA (Figure 8). Significantly higher percentage of cells in the culture of medium containing extracts of scaffold 3 were observed while a significant decrease in the percentage of cells was noted in the culture with extracts of scaffold 4 (Figure 9). Cells cultured in the scaffold extracts showed 100% viability during the 5 days culture period. Clustering effects of the cells correlating to the higher number of cells in medium with extracts of scaffolds 2 and 3 indicates the adhesive behavior of the MG63 cells.


Development and characterization of novel porous 3D alginate-cockle shell powder nanobiocomposite bone scaffold.

Bharatham BH, Abu Bakar MZ, Perimal EK, Yusof LM, Hamid M - Biomed Res Int (2014)

Fluorescence stained cells grown in the medium containing leachable of scaffold 1 (a), scaffold 2 (b), scaffold 3 (c), scaffold 4 (d), scaffold 5 (e), and normal culture medium (f) taken at ×10 magnification.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109673&req=5

fig8: Fluorescence stained cells grown in the medium containing leachable of scaffold 1 (a), scaffold 2 (b), scaffold 3 (c), scaffold 4 (d), scaffold 5 (e), and normal culture medium (f) taken at ×10 magnification.
Mentions: The live/dead cell staining procedure was carried out to supplement the results from the MTT assay in which the identifications of viable cells are observed to produce a green fluorescence when the stains are incorporated with the cell's DNA (Figure 8). Significantly higher percentage of cells in the culture of medium containing extracts of scaffold 3 were observed while a significant decrease in the percentage of cells was noted in the culture with extracts of scaffold 4 (Figure 9). Cells cultured in the scaffold extracts showed 100% viability during the 5 days culture period. Clustering effects of the cells correlating to the higher number of cells in medium with extracts of scaffolds 2 and 3 indicates the adhesive behavior of the MG63 cells.

Bottom Line: The scaffold was developed in varying composition mixture of Alg-nCP and characterized using various evaluation techniques as well as preliminary in vitro studies on MG63 human osteoblast cells.Morphological observations using SEM revealed variations in structures with the use of different Alg-nCP composition ratios.All the developed scaffolds showed a porous structure with pore sizes ideal for facilitating new bone growth; however, not all combination mixtures showed subsequent favorable characteristics to be used for biological applications.

View Article: PubMed Central - PubMed

Affiliation: Biomedical Sciences Programme, School of Diagnostic and Applied Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia ; Department of Veterinary Preclinical Sciences, Faculty of Veterinary Medicine, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor Darul Ehsan, Malaysia.

ABSTRACT
A novel porous three-dimensional bone scaffold was developed using a natural polymer (alginate/Alg) in combination with a naturally obtained biomineral (nano cockle shell powder/nCP) through lyophilization techniques. The scaffold was developed in varying composition mixture of Alg-nCP and characterized using various evaluation techniques as well as preliminary in vitro studies on MG63 human osteoblast cells. Morphological observations using SEM revealed variations in structures with the use of different Alg-nCP composition ratios. All the developed scaffolds showed a porous structure with pore sizes ideal for facilitating new bone growth; however, not all combination mixtures showed subsequent favorable characteristics to be used for biological applications. Scaffolds produced using the combination mixture of 40% Alg and 60% nCP produced significantly promising results in terms of mechanical strength, degradation rate, and increased cell proliferation rates making it potentially the optimum composition mixture of Alg-nCP with future application prospects.

Show MeSH
Related in: MedlinePlus