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Sperm nuclear vacuoles in relation to acrosome reactions and sperm motility.

Komiya A, Kawauchi Y, Kato T, Watanabe A, Tanii I, Fuse H - ScientificWorldJournal (2014)

Bottom Line: The mean %LNVs were 2.4% in group I, 5.8% in group II, and 9.8% in group III.These values were significantly different from each other (P < 0.001, paired t-test).There were no correlations between the %LNV values and the Acrobeads scores.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama, 2630 Sugitani, Toyama-shi, Toyama 930-0194, Japan.

ABSTRACT
We investigated sperm nuclear vacuolation in relation to acrosome reactions and the maintenance of sperm motility. Thirty male patients who visited our Male Infertility Clinic were enrolled. These patients underwent conventional semen analyses, Acrobeads tests, and high-magnification observation of the sperm head to evaluate the degree of nuclear vacuolation on the Acrobeads test scoring after 24 hours of incubation. The presence of acrosome reactions was evaluated using the Acrobeads test. The spermatozoa were classified into three groups: (I) those bound to MH61-beads, (II) motile spermatozoa that did not bind to MH61-beads, and (III) immotile spermatozoa that did not bind to MH61-beads. The percentage of spermatozoa with large nuclear vacuoles (%LNV) was compared between the three groups. The degree of sperm nuclear vacuolation was evaluated in 17,992 ejaculated spermatozoa. The mean %LNVs were 2.4% in group I, 5.8% in group II, and 9.8% in group III. These values were significantly different from each other (P < 0.001, paired t-test). There were no correlations between the %LNV values and the Acrobeads scores. In conclusion, the degree of sperm nuclear vacuolation was significantly lower in the acrosome-reacted spermatozoa and spermatozoa with maintained motility, and higher in the immotile spermatozoa that did not bind to MH61-beads.

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High-magnification observation of a sperm head (×600). M: MH61-bead; *spermatozoa bound to an MH61-bead without large nuclear vacuoles; **spermatozoa bound to an MH61-bead with large nuclear vacuoles; #spermatozoa not bound to MH61-beads without large nuclear vacuoles; ##spermatozoa not bound to MH61-beads with large nuclear vacuoles. The arrows indicate sperm large nuclear vacuoles.
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fig1: High-magnification observation of a sperm head (×600). M: MH61-bead; *spermatozoa bound to an MH61-bead without large nuclear vacuoles; **spermatozoa bound to an MH61-bead with large nuclear vacuoles; #spermatozoa not bound to MH61-beads without large nuclear vacuoles; ##spermatozoa not bound to MH61-beads with large nuclear vacuoles. The arrows indicate sperm large nuclear vacuoles.

Mentions: The spermatozoa placed on a glass bottom dish were analyzed at 3,700× using an inverted microscope equipped with Nomarski differential interference contrast optics (IX71, Olympus, Tokyo) and a video system (FX630, Olympus, Tokyo). A 60-× (1.42 numerical aperture) objective lens was used with oil. Images of the spermatozoa were captured and stored on a video system using an image-filing software program, FlvFs (Flovel, Tokyo). We spent 30 to 60 minutes capturing and analyzing the images of each ejaculate. The spermatozoa were classified into three groups: (I) those bound to MH61-beads after the acrosome reaction at 24 hours of incubation regardless of the motility, (II) motile spermatozoa that did not bind to MH61-beads, and (III) immotile spermatozoa that did not bind to MH61-beads. At least 500 spermatozoa per ejaculate and 100 spermatozoa per each group were evaluated using the high-magnification microscope [14]. A nuclear vacuole was defined as “large” if the maximum diameter of the vacuole was more than 50% of the width of the sperm head [14]. Using this system, we evaluated large nuclear vacuoles (LNVs) not only in motile spermatozoa but also in immotile spermatozoa (Figure 1).


Sperm nuclear vacuoles in relation to acrosome reactions and sperm motility.

Komiya A, Kawauchi Y, Kato T, Watanabe A, Tanii I, Fuse H - ScientificWorldJournal (2014)

High-magnification observation of a sperm head (×600). M: MH61-bead; *spermatozoa bound to an MH61-bead without large nuclear vacuoles; **spermatozoa bound to an MH61-bead with large nuclear vacuoles; #spermatozoa not bound to MH61-beads without large nuclear vacuoles; ##spermatozoa not bound to MH61-beads with large nuclear vacuoles. The arrows indicate sperm large nuclear vacuoles.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4109670&req=5

fig1: High-magnification observation of a sperm head (×600). M: MH61-bead; *spermatozoa bound to an MH61-bead without large nuclear vacuoles; **spermatozoa bound to an MH61-bead with large nuclear vacuoles; #spermatozoa not bound to MH61-beads without large nuclear vacuoles; ##spermatozoa not bound to MH61-beads with large nuclear vacuoles. The arrows indicate sperm large nuclear vacuoles.
Mentions: The spermatozoa placed on a glass bottom dish were analyzed at 3,700× using an inverted microscope equipped with Nomarski differential interference contrast optics (IX71, Olympus, Tokyo) and a video system (FX630, Olympus, Tokyo). A 60-× (1.42 numerical aperture) objective lens was used with oil. Images of the spermatozoa were captured and stored on a video system using an image-filing software program, FlvFs (Flovel, Tokyo). We spent 30 to 60 minutes capturing and analyzing the images of each ejaculate. The spermatozoa were classified into three groups: (I) those bound to MH61-beads after the acrosome reaction at 24 hours of incubation regardless of the motility, (II) motile spermatozoa that did not bind to MH61-beads, and (III) immotile spermatozoa that did not bind to MH61-beads. At least 500 spermatozoa per ejaculate and 100 spermatozoa per each group were evaluated using the high-magnification microscope [14]. A nuclear vacuole was defined as “large” if the maximum diameter of the vacuole was more than 50% of the width of the sperm head [14]. Using this system, we evaluated large nuclear vacuoles (LNVs) not only in motile spermatozoa but also in immotile spermatozoa (Figure 1).

Bottom Line: The mean %LNVs were 2.4% in group I, 5.8% in group II, and 9.8% in group III.These values were significantly different from each other (P < 0.001, paired t-test).There were no correlations between the %LNV values and the Acrobeads scores.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama, 2630 Sugitani, Toyama-shi, Toyama 930-0194, Japan.

ABSTRACT
We investigated sperm nuclear vacuolation in relation to acrosome reactions and the maintenance of sperm motility. Thirty male patients who visited our Male Infertility Clinic were enrolled. These patients underwent conventional semen analyses, Acrobeads tests, and high-magnification observation of the sperm head to evaluate the degree of nuclear vacuolation on the Acrobeads test scoring after 24 hours of incubation. The presence of acrosome reactions was evaluated using the Acrobeads test. The spermatozoa were classified into three groups: (I) those bound to MH61-beads, (II) motile spermatozoa that did not bind to MH61-beads, and (III) immotile spermatozoa that did not bind to MH61-beads. The percentage of spermatozoa with large nuclear vacuoles (%LNV) was compared between the three groups. The degree of sperm nuclear vacuolation was evaluated in 17,992 ejaculated spermatozoa. The mean %LNVs were 2.4% in group I, 5.8% in group II, and 9.8% in group III. These values were significantly different from each other (P < 0.001, paired t-test). There were no correlations between the %LNV values and the Acrobeads scores. In conclusion, the degree of sperm nuclear vacuolation was significantly lower in the acrosome-reacted spermatozoa and spermatozoa with maintained motility, and higher in the immotile spermatozoa that did not bind to MH61-beads.

Show MeSH
Related in: MedlinePlus