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Small RNAs in mycobacteria: an unfolding story.

Haning K, Cho SH, Contreras LM - Front Cell Infect Microbiol (2014)

Bottom Line: Mycobacteria represent a class of powerful pathogens, including those causing tuberculosis and leprosy, which continue to be worldwide health challenges.Characterization of these sRNAs is only beginning, but differential expression under environmental stresses suggests relevance to mycobacterial pathogenesis.This review provides a comprehensive overview of the current knowledge of sRNAs in mycobacteria, including historical perspective and techniques used for identification and characterization.

View Article: PubMed Central - PubMed

Affiliation: McKetta Department of Chemical Engineering, Cockrell School of Engineering, The University of Texas at Austin Austin, TX, USA.

ABSTRACT
Mycobacteria represent a class of powerful pathogens, including those causing tuberculosis and leprosy, which continue to be worldwide health challenges. In the last 20 years, an abundance of non-coding, small RNAs (sRNAs) have been discovered in model bacteria and gained significant attention as regulators of cellular responses, including pathogenesis. Naturally, a search in mycobacteria followed, revealing over 200 sRNAs thus far. Characterization of these sRNAs is only beginning, but differential expression under environmental stresses suggests relevance to mycobacterial pathogenesis. This review provides a comprehensive overview of the current knowledge of sRNAs in mycobacteria, including historical perspective and techniques used for identification and characterization.

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sRNA search and characterization. Discovery of sRNAs often begins with large-scale computational searches followed by experimental validation. Functional characterization of confirmed candidates identifies their gene or protein targets and mechanistic studies elucidate their methods of action. Finally, sRNAs can be used in engineering efforts to develop useful applications from synthetic elements to medical treatments.
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Figure 2: sRNA search and characterization. Discovery of sRNAs often begins with large-scale computational searches followed by experimental validation. Functional characterization of confirmed candidates identifies their gene or protein targets and mechanistic studies elucidate their methods of action. Finally, sRNAs can be used in engineering efforts to develop useful applications from synthetic elements to medical treatments.

Mentions: With improved mechanistic understanding, there is enormous interest in inhibiting and/or mimicking expression of natural sRNA actions to achieve desired changes in targeted genes (Na et al., 2013; Vazquez-Anderson and Contreras, 2013). Figure 2 summarizes the large-scale sRNA searches, validation experiments, mechanistic and functional studies, and ultimately, targeting efforts. In parallel with such progress in model organisms, investigations emerged to follow this path in more unique classes of bacteria.


Small RNAs in mycobacteria: an unfolding story.

Haning K, Cho SH, Contreras LM - Front Cell Infect Microbiol (2014)

sRNA search and characterization. Discovery of sRNAs often begins with large-scale computational searches followed by experimental validation. Functional characterization of confirmed candidates identifies their gene or protein targets and mechanistic studies elucidate their methods of action. Finally, sRNAs can be used in engineering efforts to develop useful applications from synthetic elements to medical treatments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109619&req=5

Figure 2: sRNA search and characterization. Discovery of sRNAs often begins with large-scale computational searches followed by experimental validation. Functional characterization of confirmed candidates identifies their gene or protein targets and mechanistic studies elucidate their methods of action. Finally, sRNAs can be used in engineering efforts to develop useful applications from synthetic elements to medical treatments.
Mentions: With improved mechanistic understanding, there is enormous interest in inhibiting and/or mimicking expression of natural sRNA actions to achieve desired changes in targeted genes (Na et al., 2013; Vazquez-Anderson and Contreras, 2013). Figure 2 summarizes the large-scale sRNA searches, validation experiments, mechanistic and functional studies, and ultimately, targeting efforts. In parallel with such progress in model organisms, investigations emerged to follow this path in more unique classes of bacteria.

Bottom Line: Mycobacteria represent a class of powerful pathogens, including those causing tuberculosis and leprosy, which continue to be worldwide health challenges.Characterization of these sRNAs is only beginning, but differential expression under environmental stresses suggests relevance to mycobacterial pathogenesis.This review provides a comprehensive overview of the current knowledge of sRNAs in mycobacteria, including historical perspective and techniques used for identification and characterization.

View Article: PubMed Central - PubMed

Affiliation: McKetta Department of Chemical Engineering, Cockrell School of Engineering, The University of Texas at Austin Austin, TX, USA.

ABSTRACT
Mycobacteria represent a class of powerful pathogens, including those causing tuberculosis and leprosy, which continue to be worldwide health challenges. In the last 20 years, an abundance of non-coding, small RNAs (sRNAs) have been discovered in model bacteria and gained significant attention as regulators of cellular responses, including pathogenesis. Naturally, a search in mycobacteria followed, revealing over 200 sRNAs thus far. Characterization of these sRNAs is only beginning, but differential expression under environmental stresses suggests relevance to mycobacterial pathogenesis. This review provides a comprehensive overview of the current knowledge of sRNAs in mycobacteria, including historical perspective and techniques used for identification and characterization.

Show MeSH
Related in: MedlinePlus