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Ultra-sensitive optical oxygen sensors for characterization of nearly anoxic systems.

Lehner P, Staudinger C, Borisov SM, Klimant I - Nat Commun (2014)

Bottom Line: The sensitivity of the new sensors is improved up to 20-fold compared with state-of-the-art analogues.The limits of detection are as low as 5 p.p.b., volume in gas phase under atmospheric pressure or 7 pM in solution.The sensors enable completely new applications for monitoring of oxygen in previously inaccessible concentration ranges.

View Article: PubMed Central - PubMed

Affiliation: Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology, NAWI Graz, Stremayrgasse 9, 8010 Graz, Austria.

ABSTRACT
Oxygen quantification in trace amounts is essential in many fields of science and technology. Optical oxygen sensors proved invaluable tools for oxygen measurements in a broad concentration range, but until now neither optical nor electrochemical oxygen sensors were able to quantify oxygen in the sub-nanomolar concentration range. Herein we present new optical oxygen-sensing materials with unmatched sensitivity. They rely on the combination of ultra-long decaying (several 100 ms lifetime) phosphorescent boron- and aluminium-chelates, and highly oxygen-permeable and chemically stable perfluorinated polymers. The sensitivity of the new sensors is improved up to 20-fold compared with state-of-the-art analogues. The limits of detection are as low as 5 p.p.b., volume in gas phase under atmospheric pressure or 7 pM in solution. The sensors enable completely new applications for monitoring of oxygen in previously inaccessible concentration ranges.

No MeSH data available.


Related in: MedlinePlus

Real-time monitoring of oxygen levels during enzymatic oxygen consumptionThree trace sensors with overlapping ranges are used to monitor oxidation of glucose catalyzed by glucose oxidase. A running average of 3 data points was used for all sensors. Whereas the reference sensors fail to resolve below 2-10 nM, the new ultra trace sensor can reliably monitor oxygen even at much lower concentrations (insert).
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Figure 4: Real-time monitoring of oxygen levels during enzymatic oxygen consumptionThree trace sensors with overlapping ranges are used to monitor oxidation of glucose catalyzed by glucose oxidase. A running average of 3 data points was used for all sensors. Whereas the reference sensors fail to resolve below 2-10 nM, the new ultra trace sensor can reliably monitor oxygen even at much lower concentrations (insert).

Mentions: To illustrate potential applications of these trace oxygen sensors, the kinetics of oxygen consumption during enzymatic oxidation of glucose was monitored as it approached anoxic conditions (Fig. 4). Aqueous solutions of glucose oxidase (GOx), catalase and glucose were combined in a closed stirred vessel and the oxygen concentration was monitored with a set of sensors with overlapping ranges. Additionally to the Al(HPhNPF)3/Hyflon® sensor two other oxygen sensors were used: a commercially available trace sensor from Pyroscience GmbH and a sensor based on Pd(II)-5,10,15,20-meso-tetrakis-(2,3,4,5,6-pentafluorphenyl)-porphyrin in Hyflon® which is comparable in sensitivity to previously published sensors.31Figure 4 shows the high resolution of the new sensors in the ultra trace region, whereas the other sensors fail to resolve oxygen concentrations below 1 nM. The insert shows a mono exponential fit of the ultra trace region corresponding to purely diffusion-controlled depletion of oxygen.


Ultra-sensitive optical oxygen sensors for characterization of nearly anoxic systems.

Lehner P, Staudinger C, Borisov SM, Klimant I - Nat Commun (2014)

Real-time monitoring of oxygen levels during enzymatic oxygen consumptionThree trace sensors with overlapping ranges are used to monitor oxidation of glucose catalyzed by glucose oxidase. A running average of 3 data points was used for all sensors. Whereas the reference sensors fail to resolve below 2-10 nM, the new ultra trace sensor can reliably monitor oxygen even at much lower concentrations (insert).
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4109599&req=5

Figure 4: Real-time monitoring of oxygen levels during enzymatic oxygen consumptionThree trace sensors with overlapping ranges are used to monitor oxidation of glucose catalyzed by glucose oxidase. A running average of 3 data points was used for all sensors. Whereas the reference sensors fail to resolve below 2-10 nM, the new ultra trace sensor can reliably monitor oxygen even at much lower concentrations (insert).
Mentions: To illustrate potential applications of these trace oxygen sensors, the kinetics of oxygen consumption during enzymatic oxidation of glucose was monitored as it approached anoxic conditions (Fig. 4). Aqueous solutions of glucose oxidase (GOx), catalase and glucose were combined in a closed stirred vessel and the oxygen concentration was monitored with a set of sensors with overlapping ranges. Additionally to the Al(HPhNPF)3/Hyflon® sensor two other oxygen sensors were used: a commercially available trace sensor from Pyroscience GmbH and a sensor based on Pd(II)-5,10,15,20-meso-tetrakis-(2,3,4,5,6-pentafluorphenyl)-porphyrin in Hyflon® which is comparable in sensitivity to previously published sensors.31Figure 4 shows the high resolution of the new sensors in the ultra trace region, whereas the other sensors fail to resolve oxygen concentrations below 1 nM. The insert shows a mono exponential fit of the ultra trace region corresponding to purely diffusion-controlled depletion of oxygen.

Bottom Line: The sensitivity of the new sensors is improved up to 20-fold compared with state-of-the-art analogues.The limits of detection are as low as 5 p.p.b., volume in gas phase under atmospheric pressure or 7 pM in solution.The sensors enable completely new applications for monitoring of oxygen in previously inaccessible concentration ranges.

View Article: PubMed Central - PubMed

Affiliation: Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology, NAWI Graz, Stremayrgasse 9, 8010 Graz, Austria.

ABSTRACT
Oxygen quantification in trace amounts is essential in many fields of science and technology. Optical oxygen sensors proved invaluable tools for oxygen measurements in a broad concentration range, but until now neither optical nor electrochemical oxygen sensors were able to quantify oxygen in the sub-nanomolar concentration range. Herein we present new optical oxygen-sensing materials with unmatched sensitivity. They rely on the combination of ultra-long decaying (several 100 ms lifetime) phosphorescent boron- and aluminium-chelates, and highly oxygen-permeable and chemically stable perfluorinated polymers. The sensitivity of the new sensors is improved up to 20-fold compared with state-of-the-art analogues. The limits of detection are as low as 5 p.p.b., volume in gas phase under atmospheric pressure or 7 pM in solution. The sensors enable completely new applications for monitoring of oxygen in previously inaccessible concentration ranges.

No MeSH data available.


Related in: MedlinePlus