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Electrical stimulation counteracts muscle decline in seniors.

Kern H, Barberi L, Löfler S, Sbardella S, Burggraf S, Fruhmann H, Carraro U, Mosole S, Sarabon N, Vogelauer M, Mayr W, Krenn M, Cvecka J, Romanello V, Pietrangelo L, Protasi F, Sandri M, Zampieri S, Musaro A - Front Aging Neurosci (2014)

Bottom Line: We analyzed, at functional, structural, and molecular level, the effects of ES training on healthy seniors with normal life style, without routine sport activity.ES also induced up-regulation of relevant markers of differentiating satellite cells and of extracellular matrix remodeling, which might guarantee shape and mechanical forces of trained skeletal muscle as well as maintenance of satellite cell function, reducing fibrosis.Our data provide evidence that ES is a safe method to counteract muscle decline associated with aging.

View Article: PubMed Central - PubMed

Affiliation: Institute of Physical Medicine and Rehabilitation, Wilhelminenspital , Vienna , Austria ; Ludwig Boltzmann Institute of Electrical Stimulation and Physical Rehabilitation , Vienna , Austria.

ABSTRACT
The loss in muscle mass coupled with a decrease in specific force and shift in fiber composition are hallmarks of aging. Training and regular exercise attenuate the signs of sarcopenia. However, pathologic conditions limit the ability to perform physical exercise. We addressed whether electrical stimulation (ES) is an alternative intervention to improve muscle recovery and defined the molecular mechanism associated with improvement in muscle structure and function. We analyzed, at functional, structural, and molecular level, the effects of ES training on healthy seniors with normal life style, without routine sport activity. ES was able to improve muscle torque and functional performances of seniors and increased the size of fast muscle fibers. At molecular level, ES induced up-regulation of IGF-1 and modulation of MuRF-1, a muscle-specific atrophy-related gene. ES also induced up-regulation of relevant markers of differentiating satellite cells and of extracellular matrix remodeling, which might guarantee shape and mechanical forces of trained skeletal muscle as well as maintenance of satellite cell function, reducing fibrosis. Our data provide evidence that ES is a safe method to counteract muscle decline associated with aging.

No MeSH data available.


Related in: MedlinePlus

Electrical stimulation promotes ECM remodeling. Real time PCR analysis for Collagen I, Collagen III, Collagen VI, and miR-29. Data are represented as average ± SEM. n = 16. *p < 0.05; ***p < 0.0005.
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Figure 4: Electrical stimulation promotes ECM remodeling. Real time PCR analysis for Collagen I, Collagen III, Collagen VI, and miR-29. Data are represented as average ± SEM. n = 16. *p < 0.05; ***p < 0.0005.

Mentions: To support this hypothesis, we analyzed, by real time PCR, the expression of adhesion-promoting matrix components, demonstrating a significant up-regulation of collagen types I and III in ES muscle compared to control pre-trained muscle (Figure 4). Of note, ECM represents also a niche component of satellite cells. One of the matrix components that might play a role in maintaining satellite cell function is collagen VI (Urciuolo et al., 2013). Real time PCR analysis revealed a significant increase in Collagen VI expression in ES-trained muscle compared to pre-trained muscle (Figure 4).


Electrical stimulation counteracts muscle decline in seniors.

Kern H, Barberi L, Löfler S, Sbardella S, Burggraf S, Fruhmann H, Carraro U, Mosole S, Sarabon N, Vogelauer M, Mayr W, Krenn M, Cvecka J, Romanello V, Pietrangelo L, Protasi F, Sandri M, Zampieri S, Musaro A - Front Aging Neurosci (2014)

Electrical stimulation promotes ECM remodeling. Real time PCR analysis for Collagen I, Collagen III, Collagen VI, and miR-29. Data are represented as average ± SEM. n = 16. *p < 0.05; ***p < 0.0005.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109438&req=5

Figure 4: Electrical stimulation promotes ECM remodeling. Real time PCR analysis for Collagen I, Collagen III, Collagen VI, and miR-29. Data are represented as average ± SEM. n = 16. *p < 0.05; ***p < 0.0005.
Mentions: To support this hypothesis, we analyzed, by real time PCR, the expression of adhesion-promoting matrix components, demonstrating a significant up-regulation of collagen types I and III in ES muscle compared to control pre-trained muscle (Figure 4). Of note, ECM represents also a niche component of satellite cells. One of the matrix components that might play a role in maintaining satellite cell function is collagen VI (Urciuolo et al., 2013). Real time PCR analysis revealed a significant increase in Collagen VI expression in ES-trained muscle compared to pre-trained muscle (Figure 4).

Bottom Line: We analyzed, at functional, structural, and molecular level, the effects of ES training on healthy seniors with normal life style, without routine sport activity.ES also induced up-regulation of relevant markers of differentiating satellite cells and of extracellular matrix remodeling, which might guarantee shape and mechanical forces of trained skeletal muscle as well as maintenance of satellite cell function, reducing fibrosis.Our data provide evidence that ES is a safe method to counteract muscle decline associated with aging.

View Article: PubMed Central - PubMed

Affiliation: Institute of Physical Medicine and Rehabilitation, Wilhelminenspital , Vienna , Austria ; Ludwig Boltzmann Institute of Electrical Stimulation and Physical Rehabilitation , Vienna , Austria.

ABSTRACT
The loss in muscle mass coupled with a decrease in specific force and shift in fiber composition are hallmarks of aging. Training and regular exercise attenuate the signs of sarcopenia. However, pathologic conditions limit the ability to perform physical exercise. We addressed whether electrical stimulation (ES) is an alternative intervention to improve muscle recovery and defined the molecular mechanism associated with improvement in muscle structure and function. We analyzed, at functional, structural, and molecular level, the effects of ES training on healthy seniors with normal life style, without routine sport activity. ES was able to improve muscle torque and functional performances of seniors and increased the size of fast muscle fibers. At molecular level, ES induced up-regulation of IGF-1 and modulation of MuRF-1, a muscle-specific atrophy-related gene. ES also induced up-regulation of relevant markers of differentiating satellite cells and of extracellular matrix remodeling, which might guarantee shape and mechanical forces of trained skeletal muscle as well as maintenance of satellite cell function, reducing fibrosis. Our data provide evidence that ES is a safe method to counteract muscle decline associated with aging.

No MeSH data available.


Related in: MedlinePlus