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Electrical stimulation counteracts muscle decline in seniors.

Kern H, Barberi L, Löfler S, Sbardella S, Burggraf S, Fruhmann H, Carraro U, Mosole S, Sarabon N, Vogelauer M, Mayr W, Krenn M, Cvecka J, Romanello V, Pietrangelo L, Protasi F, Sandri M, Zampieri S, Musaro A - Front Aging Neurosci (2014)

Bottom Line: We analyzed, at functional, structural, and molecular level, the effects of ES training on healthy seniors with normal life style, without routine sport activity.ES also induced up-regulation of relevant markers of differentiating satellite cells and of extracellular matrix remodeling, which might guarantee shape and mechanical forces of trained skeletal muscle as well as maintenance of satellite cell function, reducing fibrosis.Our data provide evidence that ES is a safe method to counteract muscle decline associated with aging.

View Article: PubMed Central - PubMed

Affiliation: Institute of Physical Medicine and Rehabilitation, Wilhelminenspital , Vienna , Austria ; Ludwig Boltzmann Institute of Electrical Stimulation and Physical Rehabilitation , Vienna , Austria.

ABSTRACT
The loss in muscle mass coupled with a decrease in specific force and shift in fiber composition are hallmarks of aging. Training and regular exercise attenuate the signs of sarcopenia. However, pathologic conditions limit the ability to perform physical exercise. We addressed whether electrical stimulation (ES) is an alternative intervention to improve muscle recovery and defined the molecular mechanism associated with improvement in muscle structure and function. We analyzed, at functional, structural, and molecular level, the effects of ES training on healthy seniors with normal life style, without routine sport activity. ES was able to improve muscle torque and functional performances of seniors and increased the size of fast muscle fibers. At molecular level, ES induced up-regulation of IGF-1 and modulation of MuRF-1, a muscle-specific atrophy-related gene. ES also induced up-regulation of relevant markers of differentiating satellite cells and of extracellular matrix remodeling, which might guarantee shape and mechanical forces of trained skeletal muscle as well as maintenance of satellite cell function, reducing fibrosis. Our data provide evidence that ES is a safe method to counteract muscle decline associated with aging.

No MeSH data available.


Related in: MedlinePlus

Muscle morphology and fiber-type distribution. All muscle biopsies present well-packed myofibers, without signs of fibrosis, and inflammatory cell infiltration before (A) or after 9 weeks of training (B). The training induced an increase of either diameter and percentage of the fast-type fibers [brown stained (C,D)]. Bar 100 μm.
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Figure 1: Muscle morphology and fiber-type distribution. All muscle biopsies present well-packed myofibers, without signs of fibrosis, and inflammatory cell infiltration before (A) or after 9 weeks of training (B). The training induced an increase of either diameter and percentage of the fast-type fibers [brown stained (C,D)]. Bar 100 μm.

Mentions: We also monitored whether the aforementioned functional benefit, exerted by ES, was associated with a morphological gain. ES training maintained the overall mean myofiber diameter (Figures 1A,B; Table 2), while significantly increased the diameter of fast-type myofibers and decreased that of slow fibers type (Figures 1C,D; Table 2). Changes in fiber-type distribution were also observed, even though not significantly (Table 2).


Electrical stimulation counteracts muscle decline in seniors.

Kern H, Barberi L, Löfler S, Sbardella S, Burggraf S, Fruhmann H, Carraro U, Mosole S, Sarabon N, Vogelauer M, Mayr W, Krenn M, Cvecka J, Romanello V, Pietrangelo L, Protasi F, Sandri M, Zampieri S, Musaro A - Front Aging Neurosci (2014)

Muscle morphology and fiber-type distribution. All muscle biopsies present well-packed myofibers, without signs of fibrosis, and inflammatory cell infiltration before (A) or after 9 weeks of training (B). The training induced an increase of either diameter and percentage of the fast-type fibers [brown stained (C,D)]. Bar 100 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109438&req=5

Figure 1: Muscle morphology and fiber-type distribution. All muscle biopsies present well-packed myofibers, without signs of fibrosis, and inflammatory cell infiltration before (A) or after 9 weeks of training (B). The training induced an increase of either diameter and percentage of the fast-type fibers [brown stained (C,D)]. Bar 100 μm.
Mentions: We also monitored whether the aforementioned functional benefit, exerted by ES, was associated with a morphological gain. ES training maintained the overall mean myofiber diameter (Figures 1A,B; Table 2), while significantly increased the diameter of fast-type myofibers and decreased that of slow fibers type (Figures 1C,D; Table 2). Changes in fiber-type distribution were also observed, even though not significantly (Table 2).

Bottom Line: We analyzed, at functional, structural, and molecular level, the effects of ES training on healthy seniors with normal life style, without routine sport activity.ES also induced up-regulation of relevant markers of differentiating satellite cells and of extracellular matrix remodeling, which might guarantee shape and mechanical forces of trained skeletal muscle as well as maintenance of satellite cell function, reducing fibrosis.Our data provide evidence that ES is a safe method to counteract muscle decline associated with aging.

View Article: PubMed Central - PubMed

Affiliation: Institute of Physical Medicine and Rehabilitation, Wilhelminenspital , Vienna , Austria ; Ludwig Boltzmann Institute of Electrical Stimulation and Physical Rehabilitation , Vienna , Austria.

ABSTRACT
The loss in muscle mass coupled with a decrease in specific force and shift in fiber composition are hallmarks of aging. Training and regular exercise attenuate the signs of sarcopenia. However, pathologic conditions limit the ability to perform physical exercise. We addressed whether electrical stimulation (ES) is an alternative intervention to improve muscle recovery and defined the molecular mechanism associated with improvement in muscle structure and function. We analyzed, at functional, structural, and molecular level, the effects of ES training on healthy seniors with normal life style, without routine sport activity. ES was able to improve muscle torque and functional performances of seniors and increased the size of fast muscle fibers. At molecular level, ES induced up-regulation of IGF-1 and modulation of MuRF-1, a muscle-specific atrophy-related gene. ES also induced up-regulation of relevant markers of differentiating satellite cells and of extracellular matrix remodeling, which might guarantee shape and mechanical forces of trained skeletal muscle as well as maintenance of satellite cell function, reducing fibrosis. Our data provide evidence that ES is a safe method to counteract muscle decline associated with aging.

No MeSH data available.


Related in: MedlinePlus