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Production of conjugated linoleic and conjugated α-linolenic acid in a reconstituted skim milk-based medium by bifidobacterial strains isolated from human breast milk.

Villar-Tajadura MA, Rodríguez-Alcalá LM, Martín V, Gómez de Segura A, Rodríguez JM, Requena T, Fontecha J - Biomed Res Int (2014)

Bottom Line: These bifidobacterial strains were also able to simultaneously produce both CLA (90-105 μg mL(-1)) and CLNA (290-320 μg mL(-1)) in reconstituted skim milk.Globally, our findings suggest that these bifidobacterial strains are potential candidates for the design of new fermented dairy products naturally containing very high concentrations of these bioactive lipids.To our knowledge, this is the first study describing CLNA production and coproduction of CLA and CLNA by Bifidobacterium breve strains isolated from human milk in reconstituted skim milk.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioactividad y Análisis de Alimentos, Instituto de Investigación en Ciencias de la Alimentación CIAL (CSIC-UAM) CEI UAM+CSIC, C/Nicolás Cabrera 9, Campus de la Universidad Autónoma de Madrid (UAM), 28049 Madrid, Spain ; Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, 28040 Madrid, Spain.

ABSTRACT
Eight bifidobacterial strains isolated from human breast milk have been tested for their abilities to convert linoleic acid (LA) and α-linolenic acid (LNA) to conjugated linoleic acid (CLA) and conjugated α-linolenic acid (CLNA), respectively. These bioactive lipids display important properties that may contribute to the maintenance and improvement human health. Three selected Bifidobacterium breve strains produced CLA from LA and CLNA from LNA in MRS (160-170 and 210-230 μg mL(-1), resp.) and, also, in reconstituted skim milk (75-95 and 210-244 μg mL(-1), resp.). These bifidobacterial strains were also able to simultaneously produce both CLA (90-105 μg mL(-1)) and CLNA (290-320 μg mL(-1)) in reconstituted skim milk. Globally, our findings suggest that these bifidobacterial strains are potential candidates for the design of new fermented dairy products naturally containing very high concentrations of these bioactive lipids. To our knowledge, this is the first study describing CLNA production and coproduction of CLA and CLNA by Bifidobacterium breve strains isolated from human milk in reconstituted skim milk.

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CLA/CLNA production by selected bacteria of screening. The cultures were incubated in reconstituted skim milk containing 500 μg mL−1 free linoleic acid (LA) and 500 μg mL−1 free linolenic acid (LNA) for 24 hours under anaerobic conditions. Values are means ± SD of three independent experiments.
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fig4: CLA/CLNA production by selected bacteria of screening. The cultures were incubated in reconstituted skim milk containing 500 μg mL−1 free linoleic acid (LA) and 500 μg mL−1 free linolenic acid (LNA) for 24 hours under anaerobic conditions. Values are means ± SD of three independent experiments.

Mentions: Finally, the three selected bifidobacterial strains were tested for their ability to produce CLA and CLNA when both substrates (LA and LNA) were added to the milk-based medium. The three B. breve strains were able to simultaneously produce CLA and CLNA in these growth conditions (Figure 4). The CLA and CLNA concentrations produced were about 100 μg mL−1 of CLA and 300 μg mL−1 of CLNA (Figure 4). Interestingly, the production of both bioactive lipids was much higher when both substrates, LA and LNA, were present at the same time in the growing medium than when they were individually added (Table 4 and Figure 4).


Production of conjugated linoleic and conjugated α-linolenic acid in a reconstituted skim milk-based medium by bifidobacterial strains isolated from human breast milk.

Villar-Tajadura MA, Rodríguez-Alcalá LM, Martín V, Gómez de Segura A, Rodríguez JM, Requena T, Fontecha J - Biomed Res Int (2014)

CLA/CLNA production by selected bacteria of screening. The cultures were incubated in reconstituted skim milk containing 500 μg mL−1 free linoleic acid (LA) and 500 μg mL−1 free linolenic acid (LNA) for 24 hours under anaerobic conditions. Values are means ± SD of three independent experiments.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109426&req=5

fig4: CLA/CLNA production by selected bacteria of screening. The cultures were incubated in reconstituted skim milk containing 500 μg mL−1 free linoleic acid (LA) and 500 μg mL−1 free linolenic acid (LNA) for 24 hours under anaerobic conditions. Values are means ± SD of three independent experiments.
Mentions: Finally, the three selected bifidobacterial strains were tested for their ability to produce CLA and CLNA when both substrates (LA and LNA) were added to the milk-based medium. The three B. breve strains were able to simultaneously produce CLA and CLNA in these growth conditions (Figure 4). The CLA and CLNA concentrations produced were about 100 μg mL−1 of CLA and 300 μg mL−1 of CLNA (Figure 4). Interestingly, the production of both bioactive lipids was much higher when both substrates, LA and LNA, were present at the same time in the growing medium than when they were individually added (Table 4 and Figure 4).

Bottom Line: These bifidobacterial strains were also able to simultaneously produce both CLA (90-105 μg mL(-1)) and CLNA (290-320 μg mL(-1)) in reconstituted skim milk.Globally, our findings suggest that these bifidobacterial strains are potential candidates for the design of new fermented dairy products naturally containing very high concentrations of these bioactive lipids.To our knowledge, this is the first study describing CLNA production and coproduction of CLA and CLNA by Bifidobacterium breve strains isolated from human milk in reconstituted skim milk.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioactividad y Análisis de Alimentos, Instituto de Investigación en Ciencias de la Alimentación CIAL (CSIC-UAM) CEI UAM+CSIC, C/Nicolás Cabrera 9, Campus de la Universidad Autónoma de Madrid (UAM), 28049 Madrid, Spain ; Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, 28040 Madrid, Spain.

ABSTRACT
Eight bifidobacterial strains isolated from human breast milk have been tested for their abilities to convert linoleic acid (LA) and α-linolenic acid (LNA) to conjugated linoleic acid (CLA) and conjugated α-linolenic acid (CLNA), respectively. These bioactive lipids display important properties that may contribute to the maintenance and improvement human health. Three selected Bifidobacterium breve strains produced CLA from LA and CLNA from LNA in MRS (160-170 and 210-230 μg mL(-1), resp.) and, also, in reconstituted skim milk (75-95 and 210-244 μg mL(-1), resp.). These bifidobacterial strains were also able to simultaneously produce both CLA (90-105 μg mL(-1)) and CLNA (290-320 μg mL(-1)) in reconstituted skim milk. Globally, our findings suggest that these bifidobacterial strains are potential candidates for the design of new fermented dairy products naturally containing very high concentrations of these bioactive lipids. To our knowledge, this is the first study describing CLNA production and coproduction of CLA and CLNA by Bifidobacterium breve strains isolated from human milk in reconstituted skim milk.

Show MeSH
Related in: MedlinePlus