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Production of conjugated linoleic and conjugated α-linolenic acid in a reconstituted skim milk-based medium by bifidobacterial strains isolated from human breast milk.

Villar-Tajadura MA, Rodríguez-Alcalá LM, Martín V, Gómez de Segura A, Rodríguez JM, Requena T, Fontecha J - Biomed Res Int (2014)

Bottom Line: These bifidobacterial strains were also able to simultaneously produce both CLA (90-105 μg mL(-1)) and CLNA (290-320 μg mL(-1)) in reconstituted skim milk.Globally, our findings suggest that these bifidobacterial strains are potential candidates for the design of new fermented dairy products naturally containing very high concentrations of these bioactive lipids.To our knowledge, this is the first study describing CLNA production and coproduction of CLA and CLNA by Bifidobacterium breve strains isolated from human milk in reconstituted skim milk.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioactividad y Análisis de Alimentos, Instituto de Investigación en Ciencias de la Alimentación CIAL (CSIC-UAM) CEI UAM+CSIC, C/Nicolás Cabrera 9, Campus de la Universidad Autónoma de Madrid (UAM), 28049 Madrid, Spain ; Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, 28040 Madrid, Spain.

ABSTRACT
Eight bifidobacterial strains isolated from human breast milk have been tested for their abilities to convert linoleic acid (LA) and α-linolenic acid (LNA) to conjugated linoleic acid (CLA) and conjugated α-linolenic acid (CLNA), respectively. These bioactive lipids display important properties that may contribute to the maintenance and improvement human health. Three selected Bifidobacterium breve strains produced CLA from LA and CLNA from LNA in MRS (160-170 and 210-230 μg mL(-1), resp.) and, also, in reconstituted skim milk (75-95 and 210-244 μg mL(-1), resp.). These bifidobacterial strains were also able to simultaneously produce both CLA (90-105 μg mL(-1)) and CLNA (290-320 μg mL(-1)) in reconstituted skim milk. Globally, our findings suggest that these bifidobacterial strains are potential candidates for the design of new fermented dairy products naturally containing very high concentrations of these bioactive lipids. To our knowledge, this is the first study describing CLNA production and coproduction of CLA and CLNA by Bifidobacterium breve strains isolated from human milk in reconstituted skim milk.

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(a) Chromatogram profile assessed by gas chromatography of the fatty acid content present in the culture media obtained from B. breve M7-70 in MRS broth with 500 μg mL−1 LNA as a substrate. (b) The insert shows a blow-up of the part of the chromatogram corresponding to the CLNA isomers.
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fig2: (a) Chromatogram profile assessed by gas chromatography of the fatty acid content present in the culture media obtained from B. breve M7-70 in MRS broth with 500 μg mL−1 LNA as a substrate. (b) The insert shows a blow-up of the part of the chromatogram corresponding to the CLNA isomers.

Mentions: Production of CLNA in MRS-Cys by B. breve strains was higher than that of CLA since the concentrations found in the respective culture media were higher than 200 μg mL−1, and the LNA to CLNA conversion rate was close to 100% (Table 2). Two CLNA isomers (cis 9, trans 11, cis 15 CLNA and trans 9, trans 11, cis 15 CLNA) could be detected in the chromatogram profiles (Figure 2), and cis 9, trans 11, cis 15 CLNA (rumenic acid) accounted for approximately 80% of the total amount of CLNA in the cultures of the three strains (Table 2). The conversion of LNA to CLNA by B. breve M7-70 in MRS-Cys was faster than that of LA to CLA since CLNA production began after 2 h incubation at 37°C while CLA formation required at least 6 h of incubation to be detected (Figure 3).


Production of conjugated linoleic and conjugated α-linolenic acid in a reconstituted skim milk-based medium by bifidobacterial strains isolated from human breast milk.

Villar-Tajadura MA, Rodríguez-Alcalá LM, Martín V, Gómez de Segura A, Rodríguez JM, Requena T, Fontecha J - Biomed Res Int (2014)

(a) Chromatogram profile assessed by gas chromatography of the fatty acid content present in the culture media obtained from B. breve M7-70 in MRS broth with 500 μg mL−1 LNA as a substrate. (b) The insert shows a blow-up of the part of the chromatogram corresponding to the CLNA isomers.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109426&req=5

fig2: (a) Chromatogram profile assessed by gas chromatography of the fatty acid content present in the culture media obtained from B. breve M7-70 in MRS broth with 500 μg mL−1 LNA as a substrate. (b) The insert shows a blow-up of the part of the chromatogram corresponding to the CLNA isomers.
Mentions: Production of CLNA in MRS-Cys by B. breve strains was higher than that of CLA since the concentrations found in the respective culture media were higher than 200 μg mL−1, and the LNA to CLNA conversion rate was close to 100% (Table 2). Two CLNA isomers (cis 9, trans 11, cis 15 CLNA and trans 9, trans 11, cis 15 CLNA) could be detected in the chromatogram profiles (Figure 2), and cis 9, trans 11, cis 15 CLNA (rumenic acid) accounted for approximately 80% of the total amount of CLNA in the cultures of the three strains (Table 2). The conversion of LNA to CLNA by B. breve M7-70 in MRS-Cys was faster than that of LA to CLA since CLNA production began after 2 h incubation at 37°C while CLA formation required at least 6 h of incubation to be detected (Figure 3).

Bottom Line: These bifidobacterial strains were also able to simultaneously produce both CLA (90-105 μg mL(-1)) and CLNA (290-320 μg mL(-1)) in reconstituted skim milk.Globally, our findings suggest that these bifidobacterial strains are potential candidates for the design of new fermented dairy products naturally containing very high concentrations of these bioactive lipids.To our knowledge, this is the first study describing CLNA production and coproduction of CLA and CLNA by Bifidobacterium breve strains isolated from human milk in reconstituted skim milk.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioactividad y Análisis de Alimentos, Instituto de Investigación en Ciencias de la Alimentación CIAL (CSIC-UAM) CEI UAM+CSIC, C/Nicolás Cabrera 9, Campus de la Universidad Autónoma de Madrid (UAM), 28049 Madrid, Spain ; Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, 28040 Madrid, Spain.

ABSTRACT
Eight bifidobacterial strains isolated from human breast milk have been tested for their abilities to convert linoleic acid (LA) and α-linolenic acid (LNA) to conjugated linoleic acid (CLA) and conjugated α-linolenic acid (CLNA), respectively. These bioactive lipids display important properties that may contribute to the maintenance and improvement human health. Three selected Bifidobacterium breve strains produced CLA from LA and CLNA from LNA in MRS (160-170 and 210-230 μg mL(-1), resp.) and, also, in reconstituted skim milk (75-95 and 210-244 μg mL(-1), resp.). These bifidobacterial strains were also able to simultaneously produce both CLA (90-105 μg mL(-1)) and CLNA (290-320 μg mL(-1)) in reconstituted skim milk. Globally, our findings suggest that these bifidobacterial strains are potential candidates for the design of new fermented dairy products naturally containing very high concentrations of these bioactive lipids. To our knowledge, this is the first study describing CLNA production and coproduction of CLA and CLNA by Bifidobacterium breve strains isolated from human milk in reconstituted skim milk.

Show MeSH