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Production of conjugated linoleic and conjugated α-linolenic acid in a reconstituted skim milk-based medium by bifidobacterial strains isolated from human breast milk.

Villar-Tajadura MA, Rodríguez-Alcalá LM, Martín V, Gómez de Segura A, Rodríguez JM, Requena T, Fontecha J - Biomed Res Int (2014)

Bottom Line: These bifidobacterial strains were also able to simultaneously produce both CLA (90-105 μg mL(-1)) and CLNA (290-320 μg mL(-1)) in reconstituted skim milk.Globally, our findings suggest that these bifidobacterial strains are potential candidates for the design of new fermented dairy products naturally containing very high concentrations of these bioactive lipids.To our knowledge, this is the first study describing CLNA production and coproduction of CLA and CLNA by Bifidobacterium breve strains isolated from human milk in reconstituted skim milk.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioactividad y Análisis de Alimentos, Instituto de Investigación en Ciencias de la Alimentación CIAL (CSIC-UAM) CEI UAM+CSIC, C/Nicolás Cabrera 9, Campus de la Universidad Autónoma de Madrid (UAM), 28049 Madrid, Spain ; Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, 28040 Madrid, Spain.

ABSTRACT
Eight bifidobacterial strains isolated from human breast milk have been tested for their abilities to convert linoleic acid (LA) and α-linolenic acid (LNA) to conjugated linoleic acid (CLA) and conjugated α-linolenic acid (CLNA), respectively. These bioactive lipids display important properties that may contribute to the maintenance and improvement human health. Three selected Bifidobacterium breve strains produced CLA from LA and CLNA from LNA in MRS (160-170 and 210-230 μg mL(-1), resp.) and, also, in reconstituted skim milk (75-95 and 210-244 μg mL(-1), resp.). These bifidobacterial strains were also able to simultaneously produce both CLA (90-105 μg mL(-1)) and CLNA (290-320 μg mL(-1)) in reconstituted skim milk. Globally, our findings suggest that these bifidobacterial strains are potential candidates for the design of new fermented dairy products naturally containing very high concentrations of these bioactive lipids. To our knowledge, this is the first study describing CLNA production and coproduction of CLA and CLNA by Bifidobacterium breve strains isolated from human milk in reconstituted skim milk.

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(a) Chromatogram profile assessed by gas chromatography of the fatty acid content present in the culture media obtained from B. breve M7-70 in MRS broth with 500 μg mL−1 LA added as a substrate. (b) The insert shows a blow-up of the part of the chromatogram corresponding to the CLA isomers.
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fig1: (a) Chromatogram profile assessed by gas chromatography of the fatty acid content present in the culture media obtained from B. breve M7-70 in MRS broth with 500 μg mL−1 LA added as a substrate. (b) The insert shows a blow-up of the part of the chromatogram corresponding to the CLA isomers.

Mentions: Subsequently, each of the three selected CLA-producing strains was assayed for CLA and CLNA production by GLC determination after incubation for 24 h at 37°C in MRS-Cys and skim milk (Tables 2 and 4). The concentration of CLA produced by the selected Bifidobacterium strains reached values above 158.7 μg mL−1, indicating that the minimal conversion rate from the added LA was approximately 74%. The bacterial strains were able to produce different CLA isomers, such as cis 9, trans 11 CLA, trans 10, cis 12 CLA and trans 9, trans 11 CLA (Figure 1); among them, cis 9, trans 11 CLA (rumenic acid) was the most abundant isomer, accounting for more than 80% of the total CLA in all cases (Table 2). As it has been reported, many bacteria are inhibited by free long-chain fatty acids in the media [18]; however, B. breve M7-70 was able to grow in the presence of LA and LNA at concentrations up to 1500 and 500 μg mL−1, respectively (Table 3).


Production of conjugated linoleic and conjugated α-linolenic acid in a reconstituted skim milk-based medium by bifidobacterial strains isolated from human breast milk.

Villar-Tajadura MA, Rodríguez-Alcalá LM, Martín V, Gómez de Segura A, Rodríguez JM, Requena T, Fontecha J - Biomed Res Int (2014)

(a) Chromatogram profile assessed by gas chromatography of the fatty acid content present in the culture media obtained from B. breve M7-70 in MRS broth with 500 μg mL−1 LA added as a substrate. (b) The insert shows a blow-up of the part of the chromatogram corresponding to the CLA isomers.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109426&req=5

fig1: (a) Chromatogram profile assessed by gas chromatography of the fatty acid content present in the culture media obtained from B. breve M7-70 in MRS broth with 500 μg mL−1 LA added as a substrate. (b) The insert shows a blow-up of the part of the chromatogram corresponding to the CLA isomers.
Mentions: Subsequently, each of the three selected CLA-producing strains was assayed for CLA and CLNA production by GLC determination after incubation for 24 h at 37°C in MRS-Cys and skim milk (Tables 2 and 4). The concentration of CLA produced by the selected Bifidobacterium strains reached values above 158.7 μg mL−1, indicating that the minimal conversion rate from the added LA was approximately 74%. The bacterial strains were able to produce different CLA isomers, such as cis 9, trans 11 CLA, trans 10, cis 12 CLA and trans 9, trans 11 CLA (Figure 1); among them, cis 9, trans 11 CLA (rumenic acid) was the most abundant isomer, accounting for more than 80% of the total CLA in all cases (Table 2). As it has been reported, many bacteria are inhibited by free long-chain fatty acids in the media [18]; however, B. breve M7-70 was able to grow in the presence of LA and LNA at concentrations up to 1500 and 500 μg mL−1, respectively (Table 3).

Bottom Line: These bifidobacterial strains were also able to simultaneously produce both CLA (90-105 μg mL(-1)) and CLNA (290-320 μg mL(-1)) in reconstituted skim milk.Globally, our findings suggest that these bifidobacterial strains are potential candidates for the design of new fermented dairy products naturally containing very high concentrations of these bioactive lipids.To our knowledge, this is the first study describing CLNA production and coproduction of CLA and CLNA by Bifidobacterium breve strains isolated from human milk in reconstituted skim milk.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioactividad y Análisis de Alimentos, Instituto de Investigación en Ciencias de la Alimentación CIAL (CSIC-UAM) CEI UAM+CSIC, C/Nicolás Cabrera 9, Campus de la Universidad Autónoma de Madrid (UAM), 28049 Madrid, Spain ; Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, 28040 Madrid, Spain.

ABSTRACT
Eight bifidobacterial strains isolated from human breast milk have been tested for their abilities to convert linoleic acid (LA) and α-linolenic acid (LNA) to conjugated linoleic acid (CLA) and conjugated α-linolenic acid (CLNA), respectively. These bioactive lipids display important properties that may contribute to the maintenance and improvement human health. Three selected Bifidobacterium breve strains produced CLA from LA and CLNA from LNA in MRS (160-170 and 210-230 μg mL(-1), resp.) and, also, in reconstituted skim milk (75-95 and 210-244 μg mL(-1), resp.). These bifidobacterial strains were also able to simultaneously produce both CLA (90-105 μg mL(-1)) and CLNA (290-320 μg mL(-1)) in reconstituted skim milk. Globally, our findings suggest that these bifidobacterial strains are potential candidates for the design of new fermented dairy products naturally containing very high concentrations of these bioactive lipids. To our knowledge, this is the first study describing CLNA production and coproduction of CLA and CLNA by Bifidobacterium breve strains isolated from human milk in reconstituted skim milk.

Show MeSH