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The effects of apigenin on the expression of Fas/FasL apoptotic pathway in warm liver ischemia-reperfusion injury in rats.

Tsalkidou EG, Tsaroucha AK, Chatzaki E, Lambropoulou M, Papachristou F, Trypsianis G, Pitiakoudis M, Vaos G, Simopoulos C - Biomed Res Int (2014)

Bottom Line: The expression analysis of Fas and FasL genes was increasing during reperfusion (significantly in the group of 240 minutes of reperfusion).It was in the same group that apigenin decreased Fas receptor levels and inhibited apoptosis as confirmed by TUNEL assay and caspase 3 antibodies.The effects of apigenin in the Fas/FasL mediated pathway of apoptosis, in the hepatic ischemia-reperfusion, seem to have a protective result on the hepatic cell.

View Article: PubMed Central - PubMed

Affiliation: Second Department of Surgery, Medical School, Democritus University of Thrace, University Hospital of Alexandroupolis, 681 00 Alexandroupolis, Greece ; Laboratory of Experimental Surgery and Surgical Research, Medical School, Democritus University of Thrace, University Hospital of Alexandroupolis, 681 00 Alexandroupolis, Greece.

ABSTRACT

Background: The aim of this experimental study was to investigate the role of apigenin in liver apoptosis, in an experimental model of hepatic ischemia-reperfusion in rats.

Materials and methods: Forty-eight Wistar rats (apigenin and control groups), 14 to 16 weeks old and weighing 220 to 350 g, were used. They were all subjected to hepatic ischemia by occlusion of the hepatic artery and portal vein for 45 minutes and reperfusion was followed for 60, 120, and 240 minutes. Apigenin was administrated intraperitoneally. Liver tissues were used for the detection of apoptosis by TUNEL assay and caspase 3 antibodies. Expression analysis of Fas/FasL genes was evaluated by real time PCR.

Results: The expression analysis of Fas and FasL genes was increasing during reperfusion (significantly in the group of 240 minutes of reperfusion). It was in the same group that apigenin decreased Fas receptor levels and inhibited apoptosis as confirmed by TUNEL assay and caspase 3 antibodies.

Conclusions: The effects of apigenin in the Fas/FasL mediated pathway of apoptosis, in the hepatic ischemia-reperfusion, seem to have a protective result on the hepatic cell.

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Levels of FasL protein in groups: (a) C60, C120, and C240; (b) AP60, AP120, and AP240; and (c) C60-AP60, C120-AP120, and C240-AP240.
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fig4: Levels of FasL protein in groups: (a) C60, C120, and C240; (b) AP60, AP120, and AP240; and (c) C60-AP60, C120-AP120, and C240-AP240.

Mentions: Exactly the same procedure was followed regarding the FasL protein levels (Figure 4). In the control groups, the levels of FasL protein were increased significantly in C240 group when it was compared to C120. The same was not valid for the rest of the groups. In Figure 4(b), groups with apigenin administration are compared. Although a reduction of FasL levels was noticed 120 and 240 minutes after reperfusion, this reduction was not statistically significant. Completing the comparisons among C60-AP60, C120-AP120, and C240-AP240, a reduction of FasL levels, even not statistically significant, was observed only in the group of apigenin after 240 minutes of reperfusion (Figure 4(c)).


The effects of apigenin on the expression of Fas/FasL apoptotic pathway in warm liver ischemia-reperfusion injury in rats.

Tsalkidou EG, Tsaroucha AK, Chatzaki E, Lambropoulou M, Papachristou F, Trypsianis G, Pitiakoudis M, Vaos G, Simopoulos C - Biomed Res Int (2014)

Levels of FasL protein in groups: (a) C60, C120, and C240; (b) AP60, AP120, and AP240; and (c) C60-AP60, C120-AP120, and C240-AP240.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109422&req=5

fig4: Levels of FasL protein in groups: (a) C60, C120, and C240; (b) AP60, AP120, and AP240; and (c) C60-AP60, C120-AP120, and C240-AP240.
Mentions: Exactly the same procedure was followed regarding the FasL protein levels (Figure 4). In the control groups, the levels of FasL protein were increased significantly in C240 group when it was compared to C120. The same was not valid for the rest of the groups. In Figure 4(b), groups with apigenin administration are compared. Although a reduction of FasL levels was noticed 120 and 240 minutes after reperfusion, this reduction was not statistically significant. Completing the comparisons among C60-AP60, C120-AP120, and C240-AP240, a reduction of FasL levels, even not statistically significant, was observed only in the group of apigenin after 240 minutes of reperfusion (Figure 4(c)).

Bottom Line: The expression analysis of Fas and FasL genes was increasing during reperfusion (significantly in the group of 240 minutes of reperfusion).It was in the same group that apigenin decreased Fas receptor levels and inhibited apoptosis as confirmed by TUNEL assay and caspase 3 antibodies.The effects of apigenin in the Fas/FasL mediated pathway of apoptosis, in the hepatic ischemia-reperfusion, seem to have a protective result on the hepatic cell.

View Article: PubMed Central - PubMed

Affiliation: Second Department of Surgery, Medical School, Democritus University of Thrace, University Hospital of Alexandroupolis, 681 00 Alexandroupolis, Greece ; Laboratory of Experimental Surgery and Surgical Research, Medical School, Democritus University of Thrace, University Hospital of Alexandroupolis, 681 00 Alexandroupolis, Greece.

ABSTRACT

Background: The aim of this experimental study was to investigate the role of apigenin in liver apoptosis, in an experimental model of hepatic ischemia-reperfusion in rats.

Materials and methods: Forty-eight Wistar rats (apigenin and control groups), 14 to 16 weeks old and weighing 220 to 350 g, were used. They were all subjected to hepatic ischemia by occlusion of the hepatic artery and portal vein for 45 minutes and reperfusion was followed for 60, 120, and 240 minutes. Apigenin was administrated intraperitoneally. Liver tissues were used for the detection of apoptosis by TUNEL assay and caspase 3 antibodies. Expression analysis of Fas/FasL genes was evaluated by real time PCR.

Results: The expression analysis of Fas and FasL genes was increasing during reperfusion (significantly in the group of 240 minutes of reperfusion). It was in the same group that apigenin decreased Fas receptor levels and inhibited apoptosis as confirmed by TUNEL assay and caspase 3 antibodies.

Conclusions: The effects of apigenin in the Fas/FasL mediated pathway of apoptosis, in the hepatic ischemia-reperfusion, seem to have a protective result on the hepatic cell.

Show MeSH
Related in: MedlinePlus