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Effect of chemotherapy on the microbiota and metabolome of human milk, a case report.

Urbaniak C, McMillan A, Angelini M, Gloor GB, Sumarah M, Burton JP, Reid G - Microbiome (2014)

Bottom Line: Chemotherapy caused a significant deviation from a healthy microbial and metabolomic profile, with depletion of genera Bifidobacterium, Eubacterium, Staphylococcus and Cloacibacterium in favor of Acinetobacter, Xanthomonadaceae and Stenotrophomonas.The metabolites docosahexaenoic acid and inositol known for their beneficial effects were also decreased.With milk contents being critical for shaping infant immunity and development, consideration needs to be given to the impact of drugs administered to the mother and the long-term potential consequences for the health of the infant.

View Article: PubMed Central - HTML - PubMed

Affiliation: Lawson Health Research Institute, 268 Grosvenor Street, London, ON N6A 4V2, Canada ; Department of Microbiology & Immunology, Western University, London, ON N6A 5C1, Canada.

ABSTRACT

Background: Human milk is an important source of bacteria for the developing infant and has been shown to influence the bacterial composition of the neonatal gut, which in turn can affect disease risk later in life. Human milk is also an important source of nutrients, influencing bacterial composition but also directly affecting the host. While recent studies have emphasized the adverse effects of antibiotic therapy on the infant microbiota, the effects of maternal chemotherapy have not been previously studied. Here we report the effects of drug administration on the microbiota and metabolome of human milk.

Methods: Mature milk was collected every two weeks over a four month period from a lactating woman undergoing chemotherapy for Hodgkin's lymphoma. Mature milk was also collected from healthy lactating women for comparison. Microbial profiles were analyzed by 16S sequencing and the metabolome by gas chromatography-mass spectrometry.

Findings: Chemotherapy caused a significant deviation from a healthy microbial and metabolomic profile, with depletion of genera Bifidobacterium, Eubacterium, Staphylococcus and Cloacibacterium in favor of Acinetobacter, Xanthomonadaceae and Stenotrophomonas. The metabolites docosahexaenoic acid and inositol known for their beneficial effects were also decreased.

Conclusion: With milk contents being critical for shaping infant immunity and development, consideration needs to be given to the impact of drugs administered to the mother and the long-term potential consequences for the health of the infant.

No MeSH data available.


Related in: MedlinePlus

Principle component analysis of metabolites in breast milk at week 0 and during chemotherapy. (A) Scoreplot displaying the distribution of samples based on metabolites alone, where the distance between samples represents how similar the metabolome of those samples are. Each point represents the average of two technical replicates. (B) Loadings plot. Each point represents a metabolite. Metabolites present in a given quadrant of the loadings plot are present in highest abundance in samples present in the same quadrant of the scoreplot (A).
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Figure 5: Principle component analysis of metabolites in breast milk at week 0 and during chemotherapy. (A) Scoreplot displaying the distribution of samples based on metabolites alone, where the distance between samples represents how similar the metabolome of those samples are. Each point represents the average of two technical replicates. (B) Loadings plot. Each point represents a metabolite. Metabolites present in a given quadrant of the loadings plot are present in highest abundance in samples present in the same quadrant of the scoreplot (A).

Mentions: The metabolic profile also changed as a result of chemotherapy (Figure 5) and was similar between weeks 2 and 16, but different to that observed at week 0. A total of 226 metabolites were detected by our GC-MS method, 12 of which were significantly different between the week 0 and chemotherapy (weeks 2 to 16) groups (Table 1). Additional file 5 shows the relative abundances of all metabolites detected in milk and stripcharts in Additional file 6 show the distribution of key metabolites detected between the two groups.


Effect of chemotherapy on the microbiota and metabolome of human milk, a case report.

Urbaniak C, McMillan A, Angelini M, Gloor GB, Sumarah M, Burton JP, Reid G - Microbiome (2014)

Principle component analysis of metabolites in breast milk at week 0 and during chemotherapy. (A) Scoreplot displaying the distribution of samples based on metabolites alone, where the distance between samples represents how similar the metabolome of those samples are. Each point represents the average of two technical replicates. (B) Loadings plot. Each point represents a metabolite. Metabolites present in a given quadrant of the loadings plot are present in highest abundance in samples present in the same quadrant of the scoreplot (A).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4109383&req=5

Figure 5: Principle component analysis of metabolites in breast milk at week 0 and during chemotherapy. (A) Scoreplot displaying the distribution of samples based on metabolites alone, where the distance between samples represents how similar the metabolome of those samples are. Each point represents the average of two technical replicates. (B) Loadings plot. Each point represents a metabolite. Metabolites present in a given quadrant of the loadings plot are present in highest abundance in samples present in the same quadrant of the scoreplot (A).
Mentions: The metabolic profile also changed as a result of chemotherapy (Figure 5) and was similar between weeks 2 and 16, but different to that observed at week 0. A total of 226 metabolites were detected by our GC-MS method, 12 of which were significantly different between the week 0 and chemotherapy (weeks 2 to 16) groups (Table 1). Additional file 5 shows the relative abundances of all metabolites detected in milk and stripcharts in Additional file 6 show the distribution of key metabolites detected between the two groups.

Bottom Line: Chemotherapy caused a significant deviation from a healthy microbial and metabolomic profile, with depletion of genera Bifidobacterium, Eubacterium, Staphylococcus and Cloacibacterium in favor of Acinetobacter, Xanthomonadaceae and Stenotrophomonas.The metabolites docosahexaenoic acid and inositol known for their beneficial effects were also decreased.With milk contents being critical for shaping infant immunity and development, consideration needs to be given to the impact of drugs administered to the mother and the long-term potential consequences for the health of the infant.

View Article: PubMed Central - HTML - PubMed

Affiliation: Lawson Health Research Institute, 268 Grosvenor Street, London, ON N6A 4V2, Canada ; Department of Microbiology & Immunology, Western University, London, ON N6A 5C1, Canada.

ABSTRACT

Background: Human milk is an important source of bacteria for the developing infant and has been shown to influence the bacterial composition of the neonatal gut, which in turn can affect disease risk later in life. Human milk is also an important source of nutrients, influencing bacterial composition but also directly affecting the host. While recent studies have emphasized the adverse effects of antibiotic therapy on the infant microbiota, the effects of maternal chemotherapy have not been previously studied. Here we report the effects of drug administration on the microbiota and metabolome of human milk.

Methods: Mature milk was collected every two weeks over a four month period from a lactating woman undergoing chemotherapy for Hodgkin's lymphoma. Mature milk was also collected from healthy lactating women for comparison. Microbial profiles were analyzed by 16S sequencing and the metabolome by gas chromatography-mass spectrometry.

Findings: Chemotherapy caused a significant deviation from a healthy microbial and metabolomic profile, with depletion of genera Bifidobacterium, Eubacterium, Staphylococcus and Cloacibacterium in favor of Acinetobacter, Xanthomonadaceae and Stenotrophomonas. The metabolites docosahexaenoic acid and inositol known for their beneficial effects were also decreased.

Conclusion: With milk contents being critical for shaping infant immunity and development, consideration needs to be given to the impact of drugs administered to the mother and the long-term potential consequences for the health of the infant.

No MeSH data available.


Related in: MedlinePlus