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Healing efficacy of an EGF impregnated triple gel based wound dressing: in vitro and in vivo studies.

Khanbanha N, Atyabi F, Taheri A, Talaie F, Mahbod M, Dinarvand R - Biomed Res Int (2014)

Bottom Line: In the rheological test, prepared gels revealed viscoelastic properties and a small linear viscoelastic region of 0.166%.The application of CDP gel with incorporated EGF significantly reduced the defect on the rat's skin and enhanced epithelial healing compared with the topical application of the EGF-free CDP gel.The results clearly substantiate the beneficial effects of the topical application of CDP containing EGF in the acceleration of healthy wound healing process with less scarring.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, Iran.

ABSTRACT
To accomplish an ideal wound healing process which promotes healthy tissue growth with less scaring, a novel gel based topical drug delivery system composed of 3 different polymers chitosan, dextran sulfate, and polyvinylpyrrolidone K30 (CDP) was prepared. The physicochemical properties of the prepared gels were investigated in vitro. Gels showed a maximum swelling ratio of 50 ± 1.95 times of dried gel in PBS at pH 7.4. The swelling ratios increase in acidic and alkaline pH to 55.3 ± 1.75 and 65.5 ± 2.42, respectively. In the rheological test, prepared gels revealed viscoelastic properties and a small linear viscoelastic region of 0.166%. In vivo wound healing promoting activities of CDP gels containing 20 μg/mL EGF were evaluated on surgically induced dermal wounds in rats using pathologic examination. The application of CDP gel with incorporated EGF significantly reduced the defect on the rat's skin and enhanced epithelial healing compared with the topical application of the EGF-free CDP gel. The results clearly substantiate the beneficial effects of the topical application of CDP containing EGF in the acceleration of healthy wound healing process with less scarring.

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Histopathological reepithelialization diameter evaluation of the wounds treated with CDP gel containing EGF (DH1), CDP gel without EGF (H1), and untreated wounds.
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fig11: Histopathological reepithelialization diameter evaluation of the wounds treated with CDP gel containing EGF (DH1), CDP gel without EGF (H1), and untreated wounds.

Mentions: The reepithelialization diameter of the groups (Figure 11) treated with gels containing EGF was 1.1 ± 0.65 and 1.925 ± 1.53 at 8 and 11 days after operation, while the control group revealed 0.96 ± 0.2 and 1.175 ± 0.55 cm at 8 and 11 days after operation. In days 8 and 11 of reepithelialization improvement in diameter in group treated with CDP gel containing EGF was highest (not statistically significant). The microscopic photos of the wounds at day 11 are shown in Figure 10. Wound epithelium thickness was of quite similar values in all groups (approximately 150 μm), data not shown.


Healing efficacy of an EGF impregnated triple gel based wound dressing: in vitro and in vivo studies.

Khanbanha N, Atyabi F, Taheri A, Talaie F, Mahbod M, Dinarvand R - Biomed Res Int (2014)

Histopathological reepithelialization diameter evaluation of the wounds treated with CDP gel containing EGF (DH1), CDP gel without EGF (H1), and untreated wounds.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109371&req=5

fig11: Histopathological reepithelialization diameter evaluation of the wounds treated with CDP gel containing EGF (DH1), CDP gel without EGF (H1), and untreated wounds.
Mentions: The reepithelialization diameter of the groups (Figure 11) treated with gels containing EGF was 1.1 ± 0.65 and 1.925 ± 1.53 at 8 and 11 days after operation, while the control group revealed 0.96 ± 0.2 and 1.175 ± 0.55 cm at 8 and 11 days after operation. In days 8 and 11 of reepithelialization improvement in diameter in group treated with CDP gel containing EGF was highest (not statistically significant). The microscopic photos of the wounds at day 11 are shown in Figure 10. Wound epithelium thickness was of quite similar values in all groups (approximately 150 μm), data not shown.

Bottom Line: In the rheological test, prepared gels revealed viscoelastic properties and a small linear viscoelastic region of 0.166%.The application of CDP gel with incorporated EGF significantly reduced the defect on the rat's skin and enhanced epithelial healing compared with the topical application of the EGF-free CDP gel.The results clearly substantiate the beneficial effects of the topical application of CDP containing EGF in the acceleration of healthy wound healing process with less scarring.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, Iran.

ABSTRACT
To accomplish an ideal wound healing process which promotes healthy tissue growth with less scaring, a novel gel based topical drug delivery system composed of 3 different polymers chitosan, dextran sulfate, and polyvinylpyrrolidone K30 (CDP) was prepared. The physicochemical properties of the prepared gels were investigated in vitro. Gels showed a maximum swelling ratio of 50 ± 1.95 times of dried gel in PBS at pH 7.4. The swelling ratios increase in acidic and alkaline pH to 55.3 ± 1.75 and 65.5 ± 2.42, respectively. In the rheological test, prepared gels revealed viscoelastic properties and a small linear viscoelastic region of 0.166%. In vivo wound healing promoting activities of CDP gels containing 20 μg/mL EGF were evaluated on surgically induced dermal wounds in rats using pathologic examination. The application of CDP gel with incorporated EGF significantly reduced the defect on the rat's skin and enhanced epithelial healing compared with the topical application of the EGF-free CDP gel. The results clearly substantiate the beneficial effects of the topical application of CDP containing EGF in the acceleration of healthy wound healing process with less scarring.

Show MeSH
Related in: MedlinePlus