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Healing efficacy of an EGF impregnated triple gel based wound dressing: in vitro and in vivo studies.

Khanbanha N, Atyabi F, Taheri A, Talaie F, Mahbod M, Dinarvand R - Biomed Res Int (2014)

Bottom Line: In the rheological test, prepared gels revealed viscoelastic properties and a small linear viscoelastic region of 0.166%.The application of CDP gel with incorporated EGF significantly reduced the defect on the rat's skin and enhanced epithelial healing compared with the topical application of the EGF-free CDP gel.The results clearly substantiate the beneficial effects of the topical application of CDP containing EGF in the acceleration of healthy wound healing process with less scarring.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, Iran.

ABSTRACT
To accomplish an ideal wound healing process which promotes healthy tissue growth with less scaring, a novel gel based topical drug delivery system composed of 3 different polymers chitosan, dextran sulfate, and polyvinylpyrrolidone K30 (CDP) was prepared. The physicochemical properties of the prepared gels were investigated in vitro. Gels showed a maximum swelling ratio of 50 ± 1.95 times of dried gel in PBS at pH 7.4. The swelling ratios increase in acidic and alkaline pH to 55.3 ± 1.75 and 65.5 ± 2.42, respectively. In the rheological test, prepared gels revealed viscoelastic properties and a small linear viscoelastic region of 0.166%. In vivo wound healing promoting activities of CDP gels containing 20 μg/mL EGF were evaluated on surgically induced dermal wounds in rats using pathologic examination. The application of CDP gel with incorporated EGF significantly reduced the defect on the rat's skin and enhanced epithelial healing compared with the topical application of the EGF-free CDP gel. The results clearly substantiate the beneficial effects of the topical application of CDP containing EGF in the acceleration of healthy wound healing process with less scarring.

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Related in: MedlinePlus

Photographs of the relative size reduction of the wounds in rat. (a) Wound treated with CDP gel containing EGF, (b) wound treated with CDP gel without EGF, and (c) untreated group (control group) on days 1, 4, 8, and 11 after operation (n = 3, mean ± SD).
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fig7: Photographs of the relative size reduction of the wounds in rat. (a) Wound treated with CDP gel containing EGF, (b) wound treated with CDP gel without EGF, and (c) untreated group (control group) on days 1, 4, 8, and 11 after operation (n = 3, mean ± SD).

Mentions: According to data obtained from gel fraction and swelling studies, F4 formulation was chosen for the treatment of full thickness wounds on rats. Macroscopic appearance of wounds treated with F4 formulation of CDP gel with EGF, F4 formulation of CDP gel without EGF and untreated group (control group) are shown in Figure 7. Each wound was observed for a period of 1, 4, 8, and 11 days after operation. All rats survived in the postoperative period until sacrifice. There were no evidences of necrosis. The formulation of CDP gels with and without EGF induced no infection of the wounds, whereas the control group showed wound infection at 4 and 8 days after operation. The relative size reduction of the wounds treated with CDP gel with EGF, CDP gel without EGF, and untreated wounds is illustrated in Figure 8. At 8 and 11 days after operation, a significant size reduction in the wounds treated with CDP gel with EGF compared to CDP gel without EGF treated groups and control group was observed. Furthermore, the CDP gels with EGF gave about 90–95% of wound size reduction at 11 days after operation.


Healing efficacy of an EGF impregnated triple gel based wound dressing: in vitro and in vivo studies.

Khanbanha N, Atyabi F, Taheri A, Talaie F, Mahbod M, Dinarvand R - Biomed Res Int (2014)

Photographs of the relative size reduction of the wounds in rat. (a) Wound treated with CDP gel containing EGF, (b) wound treated with CDP gel without EGF, and (c) untreated group (control group) on days 1, 4, 8, and 11 after operation (n = 3, mean ± SD).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109371&req=5

fig7: Photographs of the relative size reduction of the wounds in rat. (a) Wound treated with CDP gel containing EGF, (b) wound treated with CDP gel without EGF, and (c) untreated group (control group) on days 1, 4, 8, and 11 after operation (n = 3, mean ± SD).
Mentions: According to data obtained from gel fraction and swelling studies, F4 formulation was chosen for the treatment of full thickness wounds on rats. Macroscopic appearance of wounds treated with F4 formulation of CDP gel with EGF, F4 formulation of CDP gel without EGF and untreated group (control group) are shown in Figure 7. Each wound was observed for a period of 1, 4, 8, and 11 days after operation. All rats survived in the postoperative period until sacrifice. There were no evidences of necrosis. The formulation of CDP gels with and without EGF induced no infection of the wounds, whereas the control group showed wound infection at 4 and 8 days after operation. The relative size reduction of the wounds treated with CDP gel with EGF, CDP gel without EGF, and untreated wounds is illustrated in Figure 8. At 8 and 11 days after operation, a significant size reduction in the wounds treated with CDP gel with EGF compared to CDP gel without EGF treated groups and control group was observed. Furthermore, the CDP gels with EGF gave about 90–95% of wound size reduction at 11 days after operation.

Bottom Line: In the rheological test, prepared gels revealed viscoelastic properties and a small linear viscoelastic region of 0.166%.The application of CDP gel with incorporated EGF significantly reduced the defect on the rat's skin and enhanced epithelial healing compared with the topical application of the EGF-free CDP gel.The results clearly substantiate the beneficial effects of the topical application of CDP containing EGF in the acceleration of healthy wound healing process with less scarring.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, Iran.

ABSTRACT
To accomplish an ideal wound healing process which promotes healthy tissue growth with less scaring, a novel gel based topical drug delivery system composed of 3 different polymers chitosan, dextran sulfate, and polyvinylpyrrolidone K30 (CDP) was prepared. The physicochemical properties of the prepared gels were investigated in vitro. Gels showed a maximum swelling ratio of 50 ± 1.95 times of dried gel in PBS at pH 7.4. The swelling ratios increase in acidic and alkaline pH to 55.3 ± 1.75 and 65.5 ± 2.42, respectively. In the rheological test, prepared gels revealed viscoelastic properties and a small linear viscoelastic region of 0.166%. In vivo wound healing promoting activities of CDP gels containing 20 μg/mL EGF were evaluated on surgically induced dermal wounds in rats using pathologic examination. The application of CDP gel with incorporated EGF significantly reduced the defect on the rat's skin and enhanced epithelial healing compared with the topical application of the EGF-free CDP gel. The results clearly substantiate the beneficial effects of the topical application of CDP containing EGF in the acceleration of healthy wound healing process with less scarring.

Show MeSH
Related in: MedlinePlus