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Healing efficacy of an EGF impregnated triple gel based wound dressing: in vitro and in vivo studies.

Khanbanha N, Atyabi F, Taheri A, Talaie F, Mahbod M, Dinarvand R - Biomed Res Int (2014)

Bottom Line: In the rheological test, prepared gels revealed viscoelastic properties and a small linear viscoelastic region of 0.166%.The application of CDP gel with incorporated EGF significantly reduced the defect on the rat's skin and enhanced epithelial healing compared with the topical application of the EGF-free CDP gel.The results clearly substantiate the beneficial effects of the topical application of CDP containing EGF in the acceleration of healthy wound healing process with less scarring.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, Iran.

ABSTRACT
To accomplish an ideal wound healing process which promotes healthy tissue growth with less scaring, a novel gel based topical drug delivery system composed of 3 different polymers chitosan, dextran sulfate, and polyvinylpyrrolidone K30 (CDP) was prepared. The physicochemical properties of the prepared gels were investigated in vitro. Gels showed a maximum swelling ratio of 50 ± 1.95 times of dried gel in PBS at pH 7.4. The swelling ratios increase in acidic and alkaline pH to 55.3 ± 1.75 and 65.5 ± 2.42, respectively. In the rheological test, prepared gels revealed viscoelastic properties and a small linear viscoelastic region of 0.166%. In vivo wound healing promoting activities of CDP gels containing 20 μg/mL EGF were evaluated on surgically induced dermal wounds in rats using pathologic examination. The application of CDP gel with incorporated EGF significantly reduced the defect on the rat's skin and enhanced epithelial healing compared with the topical application of the EGF-free CDP gel. The results clearly substantiate the beneficial effects of the topical application of CDP containing EGF in the acceleration of healthy wound healing process with less scarring.

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Related in: MedlinePlus

The strain sweep test of gel: (a) F1, (b) F2, (c) F3, (d) F4, (e) F5, (f) F6.
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fig6: The strain sweep test of gel: (a) F1, (b) F2, (c) F3, (d) F4, (e) F5, (f) F6.

Mentions: The strain sweep test shows large differences between the elastic G′ and the loss G′′ moduli, with G′ much higher than G′′ indicating higher viscoelastic properties of the prepared gel as shown in Figure 6. However the rapid decrease in modulus resulted in a very small LVR (linear viscoelastic region). Flow measurement was conducted to determine the plastic deformation stress, that is, the yield point of gels (Table 2). The portion of G′′ to G′ is defined as tangent δ, angle from 0 degrees indicating an absolute elastic gel to 90 degrees indicating an absolute viscous gel. The results show that as the strain increases, G′ decreases more rapidly than G′′ illustrating a more elastic gel transforms into a more viscous gel. Consider(3)G′′G′=tan⁡δ.


Healing efficacy of an EGF impregnated triple gel based wound dressing: in vitro and in vivo studies.

Khanbanha N, Atyabi F, Taheri A, Talaie F, Mahbod M, Dinarvand R - Biomed Res Int (2014)

The strain sweep test of gel: (a) F1, (b) F2, (c) F3, (d) F4, (e) F5, (f) F6.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109371&req=5

fig6: The strain sweep test of gel: (a) F1, (b) F2, (c) F3, (d) F4, (e) F5, (f) F6.
Mentions: The strain sweep test shows large differences between the elastic G′ and the loss G′′ moduli, with G′ much higher than G′′ indicating higher viscoelastic properties of the prepared gel as shown in Figure 6. However the rapid decrease in modulus resulted in a very small LVR (linear viscoelastic region). Flow measurement was conducted to determine the plastic deformation stress, that is, the yield point of gels (Table 2). The portion of G′′ to G′ is defined as tangent δ, angle from 0 degrees indicating an absolute elastic gel to 90 degrees indicating an absolute viscous gel. The results show that as the strain increases, G′ decreases more rapidly than G′′ illustrating a more elastic gel transforms into a more viscous gel. Consider(3)G′′G′=tan⁡δ.

Bottom Line: In the rheological test, prepared gels revealed viscoelastic properties and a small linear viscoelastic region of 0.166%.The application of CDP gel with incorporated EGF significantly reduced the defect on the rat's skin and enhanced epithelial healing compared with the topical application of the EGF-free CDP gel.The results clearly substantiate the beneficial effects of the topical application of CDP containing EGF in the acceleration of healthy wound healing process with less scarring.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, Iran.

ABSTRACT
To accomplish an ideal wound healing process which promotes healthy tissue growth with less scaring, a novel gel based topical drug delivery system composed of 3 different polymers chitosan, dextran sulfate, and polyvinylpyrrolidone K30 (CDP) was prepared. The physicochemical properties of the prepared gels were investigated in vitro. Gels showed a maximum swelling ratio of 50 ± 1.95 times of dried gel in PBS at pH 7.4. The swelling ratios increase in acidic and alkaline pH to 55.3 ± 1.75 and 65.5 ± 2.42, respectively. In the rheological test, prepared gels revealed viscoelastic properties and a small linear viscoelastic region of 0.166%. In vivo wound healing promoting activities of CDP gels containing 20 μg/mL EGF were evaluated on surgically induced dermal wounds in rats using pathologic examination. The application of CDP gel with incorporated EGF significantly reduced the defect on the rat's skin and enhanced epithelial healing compared with the topical application of the EGF-free CDP gel. The results clearly substantiate the beneficial effects of the topical application of CDP containing EGF in the acceleration of healthy wound healing process with less scarring.

Show MeSH
Related in: MedlinePlus