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Healing efficacy of an EGF impregnated triple gel based wound dressing: in vitro and in vivo studies.

Khanbanha N, Atyabi F, Taheri A, Talaie F, Mahbod M, Dinarvand R - Biomed Res Int (2014)

Bottom Line: In the rheological test, prepared gels revealed viscoelastic properties and a small linear viscoelastic region of 0.166%.The application of CDP gel with incorporated EGF significantly reduced the defect on the rat's skin and enhanced epithelial healing compared with the topical application of the EGF-free CDP gel.The results clearly substantiate the beneficial effects of the topical application of CDP containing EGF in the acceleration of healthy wound healing process with less scarring.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, Iran.

ABSTRACT
To accomplish an ideal wound healing process which promotes healthy tissue growth with less scaring, a novel gel based topical drug delivery system composed of 3 different polymers chitosan, dextran sulfate, and polyvinylpyrrolidone K30 (CDP) was prepared. The physicochemical properties of the prepared gels were investigated in vitro. Gels showed a maximum swelling ratio of 50 ± 1.95 times of dried gel in PBS at pH 7.4. The swelling ratios increase in acidic and alkaline pH to 55.3 ± 1.75 and 65.5 ± 2.42, respectively. In the rheological test, prepared gels revealed viscoelastic properties and a small linear viscoelastic region of 0.166%. In vivo wound healing promoting activities of CDP gels containing 20 μg/mL EGF were evaluated on surgically induced dermal wounds in rats using pathologic examination. The application of CDP gel with incorporated EGF significantly reduced the defect on the rat's skin and enhanced epithelial healing compared with the topical application of the EGF-free CDP gel. The results clearly substantiate the beneficial effects of the topical application of CDP containing EGF in the acceleration of healthy wound healing process with less scarring.

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DSC thermogram of physical mixture of chitosan-dextran (F3, F4, F5, and F6).
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fig5: DSC thermogram of physical mixture of chitosan-dextran (F3, F4, F5, and F6).

Mentions: The differential scanning calorimetry (DSC) thermograms of CDP gels are shown in Figure 5. An exothermic peak was observed in DSC thermogram of physical mixture of chitosan, dextran, and PVP at about 220–240°C. The thermograms of gels gave no intrinsic peak but showed an approximately similar pattern to thermogram of physical mixture of chitosan, dextran, and PVP (Figure 5).


Healing efficacy of an EGF impregnated triple gel based wound dressing: in vitro and in vivo studies.

Khanbanha N, Atyabi F, Taheri A, Talaie F, Mahbod M, Dinarvand R - Biomed Res Int (2014)

DSC thermogram of physical mixture of chitosan-dextran (F3, F4, F5, and F6).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109371&req=5

fig5: DSC thermogram of physical mixture of chitosan-dextran (F3, F4, F5, and F6).
Mentions: The differential scanning calorimetry (DSC) thermograms of CDP gels are shown in Figure 5. An exothermic peak was observed in DSC thermogram of physical mixture of chitosan, dextran, and PVP at about 220–240°C. The thermograms of gels gave no intrinsic peak but showed an approximately similar pattern to thermogram of physical mixture of chitosan, dextran, and PVP (Figure 5).

Bottom Line: In the rheological test, prepared gels revealed viscoelastic properties and a small linear viscoelastic region of 0.166%.The application of CDP gel with incorporated EGF significantly reduced the defect on the rat's skin and enhanced epithelial healing compared with the topical application of the EGF-free CDP gel.The results clearly substantiate the beneficial effects of the topical application of CDP containing EGF in the acceleration of healthy wound healing process with less scarring.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, Iran.

ABSTRACT
To accomplish an ideal wound healing process which promotes healthy tissue growth with less scaring, a novel gel based topical drug delivery system composed of 3 different polymers chitosan, dextran sulfate, and polyvinylpyrrolidone K30 (CDP) was prepared. The physicochemical properties of the prepared gels were investigated in vitro. Gels showed a maximum swelling ratio of 50 ± 1.95 times of dried gel in PBS at pH 7.4. The swelling ratios increase in acidic and alkaline pH to 55.3 ± 1.75 and 65.5 ± 2.42, respectively. In the rheological test, prepared gels revealed viscoelastic properties and a small linear viscoelastic region of 0.166%. In vivo wound healing promoting activities of CDP gels containing 20 μg/mL EGF were evaluated on surgically induced dermal wounds in rats using pathologic examination. The application of CDP gel with incorporated EGF significantly reduced the defect on the rat's skin and enhanced epithelial healing compared with the topical application of the EGF-free CDP gel. The results clearly substantiate the beneficial effects of the topical application of CDP containing EGF in the acceleration of healthy wound healing process with less scarring.

Show MeSH
Related in: MedlinePlus