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Healing efficacy of an EGF impregnated triple gel based wound dressing: in vitro and in vivo studies.

Khanbanha N, Atyabi F, Taheri A, Talaie F, Mahbod M, Dinarvand R - Biomed Res Int (2014)

Bottom Line: In the rheological test, prepared gels revealed viscoelastic properties and a small linear viscoelastic region of 0.166%.The application of CDP gel with incorporated EGF significantly reduced the defect on the rat's skin and enhanced epithelial healing compared with the topical application of the EGF-free CDP gel.The results clearly substantiate the beneficial effects of the topical application of CDP containing EGF in the acceleration of healthy wound healing process with less scarring.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, Iran.

ABSTRACT
To accomplish an ideal wound healing process which promotes healthy tissue growth with less scaring, a novel gel based topical drug delivery system composed of 3 different polymers chitosan, dextran sulfate, and polyvinylpyrrolidone K30 (CDP) was prepared. The physicochemical properties of the prepared gels were investigated in vitro. Gels showed a maximum swelling ratio of 50 ± 1.95 times of dried gel in PBS at pH 7.4. The swelling ratios increase in acidic and alkaline pH to 55.3 ± 1.75 and 65.5 ± 2.42, respectively. In the rheological test, prepared gels revealed viscoelastic properties and a small linear viscoelastic region of 0.166%. In vivo wound healing promoting activities of CDP gels containing 20 μg/mL EGF were evaluated on surgically induced dermal wounds in rats using pathologic examination. The application of CDP gel with incorporated EGF significantly reduced the defect on the rat's skin and enhanced epithelial healing compared with the topical application of the EGF-free CDP gel. The results clearly substantiate the beneficial effects of the topical application of CDP containing EGF in the acceleration of healthy wound healing process with less scarring.

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Measurement of swelling ratio of chitosan-dextran-PVP gel (F4) in acetate buffer (pH 4.5), PBS (pH 7.4), and phosphate buffer (pH 9) at 25°C at predetermined time points (n = 3, mean ± SD).
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fig2: Measurement of swelling ratio of chitosan-dextran-PVP gel (F4) in acetate buffer (pH 4.5), PBS (pH 7.4), and phosphate buffer (pH 9) at 25°C at predetermined time points (n = 3, mean ± SD).

Mentions: Water uptake of CDP gels was followed gravimetrically. The swelling curves of CDP gels in PBS pH of 7.4 at 25°C are given in Figure 1. CDP gels in PBS at pH 7.4 show high swelling ratios from 16.84 ± 1.95 for F5 to 50.03 ± 1.06 for F4. F4 showed the highest swelling ratio (50.03) in PBS at pH 7.4 after 45 minutes. Thus, F4 containing 1.6% low molecular weight chitosan, 0.2% dextran sulfate, and 0.2% PVP was chosen as an optimum sample to conduct further experiments. Swelling behavior was carried out for F4 at different pH values (4.5, 7.4, and 9) (Figure 2). Maximum swelling ratio of F4 was observed at pH 9.0 whereas lesser but significantly different swelling ratio was observed in other pH values in this study. Effect of ionic strength on the swelling ratio of the candidate gel (F4) was investigated at different concentration of PBS (pH 7.4) at 25°C (Figure 3). It was observed that as the concentration of PBS (pH 7.4) increased (ionic strength increased consequently), a decrease in gel swelling ratio occurred.


Healing efficacy of an EGF impregnated triple gel based wound dressing: in vitro and in vivo studies.

Khanbanha N, Atyabi F, Taheri A, Talaie F, Mahbod M, Dinarvand R - Biomed Res Int (2014)

Measurement of swelling ratio of chitosan-dextran-PVP gel (F4) in acetate buffer (pH 4.5), PBS (pH 7.4), and phosphate buffer (pH 9) at 25°C at predetermined time points (n = 3, mean ± SD).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109371&req=5

fig2: Measurement of swelling ratio of chitosan-dextran-PVP gel (F4) in acetate buffer (pH 4.5), PBS (pH 7.4), and phosphate buffer (pH 9) at 25°C at predetermined time points (n = 3, mean ± SD).
Mentions: Water uptake of CDP gels was followed gravimetrically. The swelling curves of CDP gels in PBS pH of 7.4 at 25°C are given in Figure 1. CDP gels in PBS at pH 7.4 show high swelling ratios from 16.84 ± 1.95 for F5 to 50.03 ± 1.06 for F4. F4 showed the highest swelling ratio (50.03) in PBS at pH 7.4 after 45 minutes. Thus, F4 containing 1.6% low molecular weight chitosan, 0.2% dextran sulfate, and 0.2% PVP was chosen as an optimum sample to conduct further experiments. Swelling behavior was carried out for F4 at different pH values (4.5, 7.4, and 9) (Figure 2). Maximum swelling ratio of F4 was observed at pH 9.0 whereas lesser but significantly different swelling ratio was observed in other pH values in this study. Effect of ionic strength on the swelling ratio of the candidate gel (F4) was investigated at different concentration of PBS (pH 7.4) at 25°C (Figure 3). It was observed that as the concentration of PBS (pH 7.4) increased (ionic strength increased consequently), a decrease in gel swelling ratio occurred.

Bottom Line: In the rheological test, prepared gels revealed viscoelastic properties and a small linear viscoelastic region of 0.166%.The application of CDP gel with incorporated EGF significantly reduced the defect on the rat's skin and enhanced epithelial healing compared with the topical application of the EGF-free CDP gel.The results clearly substantiate the beneficial effects of the topical application of CDP containing EGF in the acceleration of healthy wound healing process with less scarring.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, Iran.

ABSTRACT
To accomplish an ideal wound healing process which promotes healthy tissue growth with less scaring, a novel gel based topical drug delivery system composed of 3 different polymers chitosan, dextran sulfate, and polyvinylpyrrolidone K30 (CDP) was prepared. The physicochemical properties of the prepared gels were investigated in vitro. Gels showed a maximum swelling ratio of 50 ± 1.95 times of dried gel in PBS at pH 7.4. The swelling ratios increase in acidic and alkaline pH to 55.3 ± 1.75 and 65.5 ± 2.42, respectively. In the rheological test, prepared gels revealed viscoelastic properties and a small linear viscoelastic region of 0.166%. In vivo wound healing promoting activities of CDP gels containing 20 μg/mL EGF were evaluated on surgically induced dermal wounds in rats using pathologic examination. The application of CDP gel with incorporated EGF significantly reduced the defect on the rat's skin and enhanced epithelial healing compared with the topical application of the EGF-free CDP gel. The results clearly substantiate the beneficial effects of the topical application of CDP containing EGF in the acceleration of healthy wound healing process with less scarring.

Show MeSH
Related in: MedlinePlus