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Repair of segmental load-bearing bone defect by autologous mesenchymal stem cells and plasma-derived fibrin impregnated ceramic block results in early recovery of limb function.

Ng MH, Duski S, Tan KK, Yusof MR, Low KC, Rose IM, Mohamed Z, Bin Saim A, Idrus RB - Biomed Res Int (2014)

Bottom Line: Union was achieved significantly faster in TEB group with a radiological score of 4.50 ± 0.78 versus ALLO (1.06 ± 0.32), MIC (1.28 ± 0.24), and negative controls (0).Histologically, TEB group scored the highest percentage of new bone (82% ± 5.1%) compared to ALLO (5% ± 2.5%) and MIC (26% ± 5.2%).Biomechanically, TEB-treated tibiae achieved the highest compressive strength (43.50 ± 12.72 MPa) compared to those treated with ALLO (15.15 ± 3.57 MPa) and MIC (23.28 ± 6.14 MPa).

View Article: PubMed Central - PubMed

Affiliation: Tissue Engineering Centre, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, 56000 Kuala Lumpur, Malaysia.

ABSTRACT
Calcium phosphate-based bone substitutes have not been used to repair load-bearing bone defects due to their weak mechanical property. In this study, we reevaluated the functional outcomes of combining ceramic block with osteogenic-induced mesenchymal stem cells and platelet-rich plasma (TEB) to repair critical-sized segmental tibial defect. Comparisons were made with fresh marrow-impregnated ceramic block (MIC) and partially demineralized allogeneic bone block (ALLO). Six New Zealand White female rabbits were used in each study group and three rabbits with no implants were used as negative controls. By Day 90, 4/6 rabbits in TEB group and 2/6 in ALLO and MIC groups resumed normal gait pattern. Union was achieved significantly faster in TEB group with a radiological score of 4.50 ± 0.78 versus ALLO (1.06 ± 0.32), MIC (1.28 ± 0.24), and negative controls (0). Histologically, TEB group scored the highest percentage of new bone (82% ± 5.1%) compared to ALLO (5% ± 2.5%) and MIC (26% ± 5.2%). Biomechanically, TEB-treated tibiae achieved the highest compressive strength (43.50 ± 12.72 MPa) compared to those treated with ALLO (15.15 ± 3.57 MPa) and MIC (23.28 ± 6.14 MPa). In conclusion, TEB can repair critical-sized segmental load-bearing bone defects and restore limb function.

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Related in: MedlinePlus

Radiological changes seen in the three test groups immediately: Day 21, Day 60, and Day 90 after operation. TEB: defect bridged by uniform new bone, cut ends of cortex no longer distinguishable, graft no longer distinguishable. MIC: a slight increase in radiodensity surrounding and distinguishable from the graft (callus formation) with no bridging of cortex. ALLO: a slight increase in radiodensity surrounding and distinguishable from the graft bridging of one cortex with new bone formation.
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fig2: Radiological changes seen in the three test groups immediately: Day 21, Day 60, and Day 90 after operation. TEB: defect bridged by uniform new bone, cut ends of cortex no longer distinguishable, graft no longer distinguishable. MIC: a slight increase in radiodensity surrounding and distinguishable from the graft (callus formation) with no bridging of cortex. ALLO: a slight increase in radiodensity surrounding and distinguishable from the graft bridging of one cortex with new bone formation.

Mentions: Radiographs of the test and control tibiae are shown in Figure 2 and the score results are summarized in Table 2. No observable change was noted up to Day 7 across all groups. However, from Day 21 onwards, TEB score was the highest in mean radiological grade (P < 0.05). By Day 90, all rabbits in TEB showed bridging of cortices by dense radiopaque new bone and of which two achieved complete union on all sides. One rabbit in ALLO and two in MIC achieved bridging of cortices.


Repair of segmental load-bearing bone defect by autologous mesenchymal stem cells and plasma-derived fibrin impregnated ceramic block results in early recovery of limb function.

Ng MH, Duski S, Tan KK, Yusof MR, Low KC, Rose IM, Mohamed Z, Bin Saim A, Idrus RB - Biomed Res Int (2014)

Radiological changes seen in the three test groups immediately: Day 21, Day 60, and Day 90 after operation. TEB: defect bridged by uniform new bone, cut ends of cortex no longer distinguishable, graft no longer distinguishable. MIC: a slight increase in radiodensity surrounding and distinguishable from the graft (callus formation) with no bridging of cortex. ALLO: a slight increase in radiodensity surrounding and distinguishable from the graft bridging of one cortex with new bone formation.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109366&req=5

fig2: Radiological changes seen in the three test groups immediately: Day 21, Day 60, and Day 90 after operation. TEB: defect bridged by uniform new bone, cut ends of cortex no longer distinguishable, graft no longer distinguishable. MIC: a slight increase in radiodensity surrounding and distinguishable from the graft (callus formation) with no bridging of cortex. ALLO: a slight increase in radiodensity surrounding and distinguishable from the graft bridging of one cortex with new bone formation.
Mentions: Radiographs of the test and control tibiae are shown in Figure 2 and the score results are summarized in Table 2. No observable change was noted up to Day 7 across all groups. However, from Day 21 onwards, TEB score was the highest in mean radiological grade (P < 0.05). By Day 90, all rabbits in TEB showed bridging of cortices by dense radiopaque new bone and of which two achieved complete union on all sides. One rabbit in ALLO and two in MIC achieved bridging of cortices.

Bottom Line: Union was achieved significantly faster in TEB group with a radiological score of 4.50 ± 0.78 versus ALLO (1.06 ± 0.32), MIC (1.28 ± 0.24), and negative controls (0).Histologically, TEB group scored the highest percentage of new bone (82% ± 5.1%) compared to ALLO (5% ± 2.5%) and MIC (26% ± 5.2%).Biomechanically, TEB-treated tibiae achieved the highest compressive strength (43.50 ± 12.72 MPa) compared to those treated with ALLO (15.15 ± 3.57 MPa) and MIC (23.28 ± 6.14 MPa).

View Article: PubMed Central - PubMed

Affiliation: Tissue Engineering Centre, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, 56000 Kuala Lumpur, Malaysia.

ABSTRACT
Calcium phosphate-based bone substitutes have not been used to repair load-bearing bone defects due to their weak mechanical property. In this study, we reevaluated the functional outcomes of combining ceramic block with osteogenic-induced mesenchymal stem cells and platelet-rich plasma (TEB) to repair critical-sized segmental tibial defect. Comparisons were made with fresh marrow-impregnated ceramic block (MIC) and partially demineralized allogeneic bone block (ALLO). Six New Zealand White female rabbits were used in each study group and three rabbits with no implants were used as negative controls. By Day 90, 4/6 rabbits in TEB group and 2/6 in ALLO and MIC groups resumed normal gait pattern. Union was achieved significantly faster in TEB group with a radiological score of 4.50 ± 0.78 versus ALLO (1.06 ± 0.32), MIC (1.28 ± 0.24), and negative controls (0). Histologically, TEB group scored the highest percentage of new bone (82% ± 5.1%) compared to ALLO (5% ± 2.5%) and MIC (26% ± 5.2%). Biomechanically, TEB-treated tibiae achieved the highest compressive strength (43.50 ± 12.72 MPa) compared to those treated with ALLO (15.15 ± 3.57 MPa) and MIC (23.28 ± 6.14 MPa). In conclusion, TEB can repair critical-sized segmental load-bearing bone defects and restore limb function.

Show MeSH
Related in: MedlinePlus