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Differential expression of immunogenic proteins on virulent Mycobacterium tuberculosis clinical isolates.

Schierloh P, Klepp L, Vazquez C, Rocha RV, Blanco FC, Balboa L, López B, Ritacco V, Bigi F, Sasiain Mdel C - Biomed Res Int (2014)

Bottom Line: Recent studies have demonstrated that Mtb families found worldwide today differ in pathology, transmissibility, virulence, and development of immune response.We found that these proteins induced humoral immune responses in patients with drug-sensitive and drug-resistant tuberculosis with substantial cross-reactivity among the four proteins.Therefore, the present results describe new Mtb antigens with potential application as biomarkers of TB.

View Article: PubMed Central - PubMed

Affiliation: IMEX-CONICET, Academia Nacional de Medicina, Pacheco de Melo 3081, 1425 CABA, Argentina.

ABSTRACT
Molecular epidemiology has revealed that Mycobacterium tuberculosis (Mtb), formerly regarded as highly conserved species, displays a considerable degree of genetic variability that can influence the outcome of the disease as well as the innate and adaptive immune response. Recent studies have demonstrated that Mtb families found worldwide today differ in pathology, transmissibility, virulence, and development of immune response. By proteomic approaches seven proteins that were differentially expressed between a local clinical isolate from Latin-American-Mediterranean (LAM) and from Haarlem (H) lineages were identified. In order to analyze the immunogenic ability, recombinant Rv2241, Rv0009, Rv0407, and Rv2624c proteins were produced for testing specific antibody responses. We found that these proteins induced humoral immune responses in patients with drug-sensitive and drug-resistant tuberculosis with substantial cross-reactivity among the four proteins. Moreover, such reactivity was also correlated with anti-Mtb-cell surface IgM, but not with anti-ManLAM, anti-PPD, or anti-Mtb-surface IgG antibodies. Therefore, the present results describe new Mtb antigens with potential application as biomarkers of TB.

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Related in: MedlinePlus

Gene expression fold-change differences between LAM10406 and H12425 using RT-qPCR. Relative gene expression was calculated using the 2-ΔΔCt method with E correction, using sigA mRNA expression as reference genes and H12425 as the calibrator. Data were analyzed using a random permutation test (*P < 0.05). The bars indicate the average ratios of LAM10406/H12425 ± SD.
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fig2: Gene expression fold-change differences between LAM10406 and H12425 using RT-qPCR. Relative gene expression was calculated using the 2-ΔΔCt method with E correction, using sigA mRNA expression as reference genes and H12425 as the calibrator. Data were analyzed using a random permutation test (*P < 0.05). The bars indicate the average ratios of LAM10406/H12425 ± SD.

Mentions: The differential expression of the genes encoding the identified proteins was then analyzed by RT-qPCR. Of the seven proteins, the overexpression of five of them in LAM10406 could be validated at the transcriptional level (Figure 2). The regulation in gene expression in the nonvalidated cases could occur at a posttranscriptional level.


Differential expression of immunogenic proteins on virulent Mycobacterium tuberculosis clinical isolates.

Schierloh P, Klepp L, Vazquez C, Rocha RV, Blanco FC, Balboa L, López B, Ritacco V, Bigi F, Sasiain Mdel C - Biomed Res Int (2014)

Gene expression fold-change differences between LAM10406 and H12425 using RT-qPCR. Relative gene expression was calculated using the 2-ΔΔCt method with E correction, using sigA mRNA expression as reference genes and H12425 as the calibrator. Data were analyzed using a random permutation test (*P < 0.05). The bars indicate the average ratios of LAM10406/H12425 ± SD.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109345&req=5

fig2: Gene expression fold-change differences between LAM10406 and H12425 using RT-qPCR. Relative gene expression was calculated using the 2-ΔΔCt method with E correction, using sigA mRNA expression as reference genes and H12425 as the calibrator. Data were analyzed using a random permutation test (*P < 0.05). The bars indicate the average ratios of LAM10406/H12425 ± SD.
Mentions: The differential expression of the genes encoding the identified proteins was then analyzed by RT-qPCR. Of the seven proteins, the overexpression of five of them in LAM10406 could be validated at the transcriptional level (Figure 2). The regulation in gene expression in the nonvalidated cases could occur at a posttranscriptional level.

Bottom Line: Recent studies have demonstrated that Mtb families found worldwide today differ in pathology, transmissibility, virulence, and development of immune response.We found that these proteins induced humoral immune responses in patients with drug-sensitive and drug-resistant tuberculosis with substantial cross-reactivity among the four proteins.Therefore, the present results describe new Mtb antigens with potential application as biomarkers of TB.

View Article: PubMed Central - PubMed

Affiliation: IMEX-CONICET, Academia Nacional de Medicina, Pacheco de Melo 3081, 1425 CABA, Argentina.

ABSTRACT
Molecular epidemiology has revealed that Mycobacterium tuberculosis (Mtb), formerly regarded as highly conserved species, displays a considerable degree of genetic variability that can influence the outcome of the disease as well as the innate and adaptive immune response. Recent studies have demonstrated that Mtb families found worldwide today differ in pathology, transmissibility, virulence, and development of immune response. By proteomic approaches seven proteins that were differentially expressed between a local clinical isolate from Latin-American-Mediterranean (LAM) and from Haarlem (H) lineages were identified. In order to analyze the immunogenic ability, recombinant Rv2241, Rv0009, Rv0407, and Rv2624c proteins were produced for testing specific antibody responses. We found that these proteins induced humoral immune responses in patients with drug-sensitive and drug-resistant tuberculosis with substantial cross-reactivity among the four proteins. Moreover, such reactivity was also correlated with anti-Mtb-cell surface IgM, but not with anti-ManLAM, anti-PPD, or anti-Mtb-surface IgG antibodies. Therefore, the present results describe new Mtb antigens with potential application as biomarkers of TB.

Show MeSH
Related in: MedlinePlus