Limits...
Dehydroabietic acid derivative QC2 induces oncosis in hepatocellular carcinoma cells.

Zhang G, Jiang C, Wang Z, Chen W, Gu W, Ding Y - Biomed Res Int (2014)

Bottom Line: In this report, we investigate the inhibitory effect against HCC cells of QC2, the derivative of rosin's main components dehydroabietic acid.The detection of ROS accumulation, increased LDH release, and decreased ATP and Δψm confirmed the cell death.Dehydroabietic acid derivative QC2 activated oncosis related protein calpain to induce the damage of cytomembrane and organelles which finally lead to oncosis in HCC cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, Drum Tower Clinical Medical College of Nanjing Medical University, Nanjing, Jiangsu 210008, China.

ABSTRACT

Aim: Rosin, the traditional Chinese medicine, is reported to be able to inhibit skin cancer cell lines. In this report, we investigate the inhibitory effect against HCC cells of QC2, the derivative of rosin's main components dehydroabietic acid.

Methods: MTT assay was used to determine the cytotoxicity of QC2. Morphological changes were observed by time-lapse microscopy and transmission electron microscopy and the cytoskeleton changes were observed by laser-scanning confocal microscopy. Cytomembrane integrity and organelles damage were confirmed by detection of the reactive oxygen (ROS), lactate dehydrogenase (LDH), and mitochondrial membrane potential (Δψm). The underlying mechanism was manifested by Western blotting. The oncotic cell death was further confirmed by detection of oncosis related protein calpain.

Results: Swelling cell type and destroyed cytoskeleton were observed in QC2-treated HCC cells. Organelle damage was visualized by transmission electron microscopy. The detection of ROS accumulation, increased LDH release, and decreased ATP and Δψm confirmed the cell death. The oncotic related protein calpain was found to increase time-dependently in QC2-treated HCC cells, while its inhibitor PD150606 attenuated the cytotoxicity.

Conclusions: Dehydroabietic acid derivative QC2 activated oncosis related protein calpain to induce the damage of cytomembrane and organelles which finally lead to oncosis in HCC cells.

Show MeSH

Related in: MedlinePlus

QC2 destroyed the cytoskeleton of SMMC-7721 cells. (a) Immunostaining of cytoskeleton proteins actin and tubulin. (b) Expression of actin and tubulin in SMMC-7721 cells treated with or without QC2; GAPDH was set as internal standard to normalize loadings.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4109319&req=5

fig4: QC2 destroyed the cytoskeleton of SMMC-7721 cells. (a) Immunostaining of cytoskeleton proteins actin and tubulin. (b) Expression of actin and tubulin in SMMC-7721 cells treated with or without QC2; GAPDH was set as internal standard to normalize loadings.

Mentions: As ultrastructure examination showed fuzzy morphology at the end of QC2 treatment, we speculated that the cytoskeleton might also be affected. So we testified the cytoskeleton changes by fluorescence microscopy and Western blotting. As shown in Figure 4(a), the staining of microtubules and microfilaments weakened with the extended response time. Actin and tubulin protein were also detected and both suffered a significant decrease (Figure 4(b)).


Dehydroabietic acid derivative QC2 induces oncosis in hepatocellular carcinoma cells.

Zhang G, Jiang C, Wang Z, Chen W, Gu W, Ding Y - Biomed Res Int (2014)

QC2 destroyed the cytoskeleton of SMMC-7721 cells. (a) Immunostaining of cytoskeleton proteins actin and tubulin. (b) Expression of actin and tubulin in SMMC-7721 cells treated with or without QC2; GAPDH was set as internal standard to normalize loadings.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109319&req=5

fig4: QC2 destroyed the cytoskeleton of SMMC-7721 cells. (a) Immunostaining of cytoskeleton proteins actin and tubulin. (b) Expression of actin and tubulin in SMMC-7721 cells treated with or without QC2; GAPDH was set as internal standard to normalize loadings.
Mentions: As ultrastructure examination showed fuzzy morphology at the end of QC2 treatment, we speculated that the cytoskeleton might also be affected. So we testified the cytoskeleton changes by fluorescence microscopy and Western blotting. As shown in Figure 4(a), the staining of microtubules and microfilaments weakened with the extended response time. Actin and tubulin protein were also detected and both suffered a significant decrease (Figure 4(b)).

Bottom Line: In this report, we investigate the inhibitory effect against HCC cells of QC2, the derivative of rosin's main components dehydroabietic acid.The detection of ROS accumulation, increased LDH release, and decreased ATP and Δψm confirmed the cell death.Dehydroabietic acid derivative QC2 activated oncosis related protein calpain to induce the damage of cytomembrane and organelles which finally lead to oncosis in HCC cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, Drum Tower Clinical Medical College of Nanjing Medical University, Nanjing, Jiangsu 210008, China.

ABSTRACT

Aim: Rosin, the traditional Chinese medicine, is reported to be able to inhibit skin cancer cell lines. In this report, we investigate the inhibitory effect against HCC cells of QC2, the derivative of rosin's main components dehydroabietic acid.

Methods: MTT assay was used to determine the cytotoxicity of QC2. Morphological changes were observed by time-lapse microscopy and transmission electron microscopy and the cytoskeleton changes were observed by laser-scanning confocal microscopy. Cytomembrane integrity and organelles damage were confirmed by detection of the reactive oxygen (ROS), lactate dehydrogenase (LDH), and mitochondrial membrane potential (Δψm). The underlying mechanism was manifested by Western blotting. The oncotic cell death was further confirmed by detection of oncosis related protein calpain.

Results: Swelling cell type and destroyed cytoskeleton were observed in QC2-treated HCC cells. Organelle damage was visualized by transmission electron microscopy. The detection of ROS accumulation, increased LDH release, and decreased ATP and Δψm confirmed the cell death. The oncotic related protein calpain was found to increase time-dependently in QC2-treated HCC cells, while its inhibitor PD150606 attenuated the cytotoxicity.

Conclusions: Dehydroabietic acid derivative QC2 activated oncosis related protein calpain to induce the damage of cytomembrane and organelles which finally lead to oncosis in HCC cells.

Show MeSH
Related in: MedlinePlus