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Expression profiling of abiotic stress-inducible genes in response to multiple stresses in rice (Oryza sativa L.) varieties with contrasting level of stress tolerance.

Basu S, Roychoudhury A - Biomed Res Int (2014)

Bottom Line: Heat map data also showed differential transcript abundance in the three varieties, correlating the observation with transcript profiling.In silico analysis of the upstream regions of all the genes represented the existence of conserved sequence motifs in single or multiple copies that are indispensable to abiotic stress response.The present work will pave the way in future to select gene(s) for overexpression, so as to generate broad spectrum resistance to multiple stresses simultaneously.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop, Soil, and Environmental Sciences, University of Arkansas, 115 Plant Sciences Building, Fayetteville, AR 72701, USA.

ABSTRACT
The present study considered transcriptional profiles and protein expression analyses from shoot and/or root tissues under three abiotic stress conditions, namely, salinity, dehydration, and cold, as well as following exogenous abscisic acid treatment, at different time points of stress exposure in three indica rice varieties, IR-29 (salt sensitive), Pokkali, and Nonabokra (both salt tolerant). The candidate genes chosen for expression studies were HKT-1, SOS-3, NHX-1, SAPK5, SAPK7, NAC-1, Rab16A, OSBZ8, DREBP2, CRT/DREBP, WRKY24, and WRKY71, along with the candidate proteins OSBZ8, SAMDC, and GST. Gene expression profile revealed considerable differences between the salt-sensitive and salt-tolerant rice varieties, as the expression in the latter was higher even at the constitutive level, whereas it was inducible only by corresponding stress signals in IR-29. Whether in roots or shoots, the transcriptional responses to different stressors peaked following 24 h of stress/ABA exposure, and the transcript levels enhanced gradually with the period of exposure. The generality of stress responses at the transcriptional level was therefore time dependent. Heat map data also showed differential transcript abundance in the three varieties, correlating the observation with transcript profiling. In silico analysis of the upstream regions of all the genes represented the existence of conserved sequence motifs in single or multiple copies that are indispensable to abiotic stress response. Overall, the transcriptome and proteome analysis undertaken in the present study indicated that genes/proteins conferring tolerance, belonging to different functional classes, were overrepresented, thus providing novel insight into the functional basis of multiple stress tolerance in indica rice varieties. The present work will pave the way in future to select gene(s) for overexpression, so as to generate broad spectrum resistance to multiple stresses simultaneously.

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Related in: MedlinePlus

Immunoblot analysis to study the protein expression in response to salinity (200 mM NaCl), PEG (20%)-mediated dehydration, ABA (100 μM), and cold (4°C) using total protein isolated from 12-day-old leaves of Nonabokra and the antibodies raised against (a) OSBZ8 (38 kDa), (b) SAMDC (38 kDa), and (c) GST (28 kDa).
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fig6: Immunoblot analysis to study the protein expression in response to salinity (200 mM NaCl), PEG (20%)-mediated dehydration, ABA (100 μM), and cold (4°C) using total protein isolated from 12-day-old leaves of Nonabokra and the antibodies raised against (a) OSBZ8 (38 kDa), (b) SAMDC (38 kDa), and (c) GST (28 kDa).

Mentions: All the three proteins examined were found to be expressed in response to salinity, ABA, drought, and cold stress. The SAMDC protein level was found to be comparatively higher with ABA treatment and particularly induced after 24 h of cold treatment. The antioxidative enzyme GST was better triggered with dehydration, cold, and ABA treatment, especially for longer duration of stress exposure. The OSBZ8 protein expression on the other hand was nearly uniform with all the stresses (Figure 6).


Expression profiling of abiotic stress-inducible genes in response to multiple stresses in rice (Oryza sativa L.) varieties with contrasting level of stress tolerance.

Basu S, Roychoudhury A - Biomed Res Int (2014)

Immunoblot analysis to study the protein expression in response to salinity (200 mM NaCl), PEG (20%)-mediated dehydration, ABA (100 μM), and cold (4°C) using total protein isolated from 12-day-old leaves of Nonabokra and the antibodies raised against (a) OSBZ8 (38 kDa), (b) SAMDC (38 kDa), and (c) GST (28 kDa).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109260&req=5

fig6: Immunoblot analysis to study the protein expression in response to salinity (200 mM NaCl), PEG (20%)-mediated dehydration, ABA (100 μM), and cold (4°C) using total protein isolated from 12-day-old leaves of Nonabokra and the antibodies raised against (a) OSBZ8 (38 kDa), (b) SAMDC (38 kDa), and (c) GST (28 kDa).
Mentions: All the three proteins examined were found to be expressed in response to salinity, ABA, drought, and cold stress. The SAMDC protein level was found to be comparatively higher with ABA treatment and particularly induced after 24 h of cold treatment. The antioxidative enzyme GST was better triggered with dehydration, cold, and ABA treatment, especially for longer duration of stress exposure. The OSBZ8 protein expression on the other hand was nearly uniform with all the stresses (Figure 6).

Bottom Line: Heat map data also showed differential transcript abundance in the three varieties, correlating the observation with transcript profiling.In silico analysis of the upstream regions of all the genes represented the existence of conserved sequence motifs in single or multiple copies that are indispensable to abiotic stress response.The present work will pave the way in future to select gene(s) for overexpression, so as to generate broad spectrum resistance to multiple stresses simultaneously.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop, Soil, and Environmental Sciences, University of Arkansas, 115 Plant Sciences Building, Fayetteville, AR 72701, USA.

ABSTRACT
The present study considered transcriptional profiles and protein expression analyses from shoot and/or root tissues under three abiotic stress conditions, namely, salinity, dehydration, and cold, as well as following exogenous abscisic acid treatment, at different time points of stress exposure in three indica rice varieties, IR-29 (salt sensitive), Pokkali, and Nonabokra (both salt tolerant). The candidate genes chosen for expression studies were HKT-1, SOS-3, NHX-1, SAPK5, SAPK7, NAC-1, Rab16A, OSBZ8, DREBP2, CRT/DREBP, WRKY24, and WRKY71, along with the candidate proteins OSBZ8, SAMDC, and GST. Gene expression profile revealed considerable differences between the salt-sensitive and salt-tolerant rice varieties, as the expression in the latter was higher even at the constitutive level, whereas it was inducible only by corresponding stress signals in IR-29. Whether in roots or shoots, the transcriptional responses to different stressors peaked following 24 h of stress/ABA exposure, and the transcript levels enhanced gradually with the period of exposure. The generality of stress responses at the transcriptional level was therefore time dependent. Heat map data also showed differential transcript abundance in the three varieties, correlating the observation with transcript profiling. In silico analysis of the upstream regions of all the genes represented the existence of conserved sequence motifs in single or multiple copies that are indispensable to abiotic stress response. Overall, the transcriptome and proteome analysis undertaken in the present study indicated that genes/proteins conferring tolerance, belonging to different functional classes, were overrepresented, thus providing novel insight into the functional basis of multiple stress tolerance in indica rice varieties. The present work will pave the way in future to select gene(s) for overexpression, so as to generate broad spectrum resistance to multiple stresses simultaneously.

Show MeSH
Related in: MedlinePlus