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Expression profiling of abiotic stress-inducible genes in response to multiple stresses in rice (Oryza sativa L.) varieties with contrasting level of stress tolerance.

Basu S, Roychoudhury A - Biomed Res Int (2014)

Bottom Line: Heat map data also showed differential transcript abundance in the three varieties, correlating the observation with transcript profiling.In silico analysis of the upstream regions of all the genes represented the existence of conserved sequence motifs in single or multiple copies that are indispensable to abiotic stress response.The present work will pave the way in future to select gene(s) for overexpression, so as to generate broad spectrum resistance to multiple stresses simultaneously.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop, Soil, and Environmental Sciences, University of Arkansas, 115 Plant Sciences Building, Fayetteville, AR 72701, USA.

ABSTRACT
The present study considered transcriptional profiles and protein expression analyses from shoot and/or root tissues under three abiotic stress conditions, namely, salinity, dehydration, and cold, as well as following exogenous abscisic acid treatment, at different time points of stress exposure in three indica rice varieties, IR-29 (salt sensitive), Pokkali, and Nonabokra (both salt tolerant). The candidate genes chosen for expression studies were HKT-1, SOS-3, NHX-1, SAPK5, SAPK7, NAC-1, Rab16A, OSBZ8, DREBP2, CRT/DREBP, WRKY24, and WRKY71, along with the candidate proteins OSBZ8, SAMDC, and GST. Gene expression profile revealed considerable differences between the salt-sensitive and salt-tolerant rice varieties, as the expression in the latter was higher even at the constitutive level, whereas it was inducible only by corresponding stress signals in IR-29. Whether in roots or shoots, the transcriptional responses to different stressors peaked following 24 h of stress/ABA exposure, and the transcript levels enhanced gradually with the period of exposure. The generality of stress responses at the transcriptional level was therefore time dependent. Heat map data also showed differential transcript abundance in the three varieties, correlating the observation with transcript profiling. In silico analysis of the upstream regions of all the genes represented the existence of conserved sequence motifs in single or multiple copies that are indispensable to abiotic stress response. Overall, the transcriptome and proteome analysis undertaken in the present study indicated that genes/proteins conferring tolerance, belonging to different functional classes, were overrepresented, thus providing novel insight into the functional basis of multiple stress tolerance in indica rice varieties. The present work will pave the way in future to select gene(s) for overexpression, so as to generate broad spectrum resistance to multiple stresses simultaneously.

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Related in: MedlinePlus

Semiquantitative RT-PCR analysis showing differential gene expression in response to ABA (100 μM) in leaf tissues of 12-day-old seedlings of IR-29 (salt sensitive) and Pokkali (salt tolerant) rice cultivars (a); comparative gene expression in the roots and leaves of 12-day-old seedlings of Nonabokra (b); the expression of each gene was compared relative to its expression in control (untreated) sample (0 h).
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fig5: Semiquantitative RT-PCR analysis showing differential gene expression in response to ABA (100 μM) in leaf tissues of 12-day-old seedlings of IR-29 (salt sensitive) and Pokkali (salt tolerant) rice cultivars (a); comparative gene expression in the roots and leaves of 12-day-old seedlings of Nonabokra (b); the expression of each gene was compared relative to its expression in control (untreated) sample (0 h).

Mentions: The inducibility by ABA was clear from the gene expression pattern in both IR-29 and Pokkali, the genes like HKT-1, NHX-1, NAC-1, SAPK5, Rab16A, DREBP2, CRT/DREBP, and WRKY71 showing better response and SAPK7 or WRKY24 with feeble induction (Figure 5(a)). The gene expression was constitutive in Pokkali, thus showing a uniform trend. In case of both roots and leaves of ABA-treated Nonabokra seedlings, the induction in expression of all the genes (except NAC-1, WRKY24, and MADS6) over control was noteworthy (Figure 5(b)).


Expression profiling of abiotic stress-inducible genes in response to multiple stresses in rice (Oryza sativa L.) varieties with contrasting level of stress tolerance.

Basu S, Roychoudhury A - Biomed Res Int (2014)

Semiquantitative RT-PCR analysis showing differential gene expression in response to ABA (100 μM) in leaf tissues of 12-day-old seedlings of IR-29 (salt sensitive) and Pokkali (salt tolerant) rice cultivars (a); comparative gene expression in the roots and leaves of 12-day-old seedlings of Nonabokra (b); the expression of each gene was compared relative to its expression in control (untreated) sample (0 h).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109260&req=5

fig5: Semiquantitative RT-PCR analysis showing differential gene expression in response to ABA (100 μM) in leaf tissues of 12-day-old seedlings of IR-29 (salt sensitive) and Pokkali (salt tolerant) rice cultivars (a); comparative gene expression in the roots and leaves of 12-day-old seedlings of Nonabokra (b); the expression of each gene was compared relative to its expression in control (untreated) sample (0 h).
Mentions: The inducibility by ABA was clear from the gene expression pattern in both IR-29 and Pokkali, the genes like HKT-1, NHX-1, NAC-1, SAPK5, Rab16A, DREBP2, CRT/DREBP, and WRKY71 showing better response and SAPK7 or WRKY24 with feeble induction (Figure 5(a)). The gene expression was constitutive in Pokkali, thus showing a uniform trend. In case of both roots and leaves of ABA-treated Nonabokra seedlings, the induction in expression of all the genes (except NAC-1, WRKY24, and MADS6) over control was noteworthy (Figure 5(b)).

Bottom Line: Heat map data also showed differential transcript abundance in the three varieties, correlating the observation with transcript profiling.In silico analysis of the upstream regions of all the genes represented the existence of conserved sequence motifs in single or multiple copies that are indispensable to abiotic stress response.The present work will pave the way in future to select gene(s) for overexpression, so as to generate broad spectrum resistance to multiple stresses simultaneously.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop, Soil, and Environmental Sciences, University of Arkansas, 115 Plant Sciences Building, Fayetteville, AR 72701, USA.

ABSTRACT
The present study considered transcriptional profiles and protein expression analyses from shoot and/or root tissues under three abiotic stress conditions, namely, salinity, dehydration, and cold, as well as following exogenous abscisic acid treatment, at different time points of stress exposure in three indica rice varieties, IR-29 (salt sensitive), Pokkali, and Nonabokra (both salt tolerant). The candidate genes chosen for expression studies were HKT-1, SOS-3, NHX-1, SAPK5, SAPK7, NAC-1, Rab16A, OSBZ8, DREBP2, CRT/DREBP, WRKY24, and WRKY71, along with the candidate proteins OSBZ8, SAMDC, and GST. Gene expression profile revealed considerable differences between the salt-sensitive and salt-tolerant rice varieties, as the expression in the latter was higher even at the constitutive level, whereas it was inducible only by corresponding stress signals in IR-29. Whether in roots or shoots, the transcriptional responses to different stressors peaked following 24 h of stress/ABA exposure, and the transcript levels enhanced gradually with the period of exposure. The generality of stress responses at the transcriptional level was therefore time dependent. Heat map data also showed differential transcript abundance in the three varieties, correlating the observation with transcript profiling. In silico analysis of the upstream regions of all the genes represented the existence of conserved sequence motifs in single or multiple copies that are indispensable to abiotic stress response. Overall, the transcriptome and proteome analysis undertaken in the present study indicated that genes/proteins conferring tolerance, belonging to different functional classes, were overrepresented, thus providing novel insight into the functional basis of multiple stress tolerance in indica rice varieties. The present work will pave the way in future to select gene(s) for overexpression, so as to generate broad spectrum resistance to multiple stresses simultaneously.

Show MeSH
Related in: MedlinePlus