Limits...
Evaluation of the anti-inflammatory and antinociceptive effects of the essential oil from leaves of Xylopia laevigata in experimental models.

Queiroz JC, Antoniolli AR, Quintans-Júnior LJ, Brito RG, Barreto RS, Costa EV, da Silva TB, Prata AP, de Lucca W, Almeida JR, Lima JT, Quintans JS - ScientificWorldJournal (2014)

Bottom Line: Thus, we investigated the possible antioxidant, antinociceptive, and anti-inflammatory effects of X. laevigata leaf essential oil (EOX) in animal models.Additionally, pretreatment with EOX, in mice, also significantly produced (P < 0.05 or P < 0.001) antinociceptive effect by reduction of nociceptive behavior (in formalin and writhing tests).The EOX showed c-Fos label in the olfactory bulb, piriform cortex, and periaqueductal gray.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Fisiologia, Universidade Federal de Sergipe, 49.000-100 São Cristóvão, SE, Brazil.

ABSTRACT
Xylopia laevigata (Annonaceae) is a medicinal plant used in folk medicine to treat pain and inflammation. Thus, we investigated the possible antioxidant, antinociceptive, and anti-inflammatory effects of X. laevigata leaf essential oil (EOX) in animal models. Our EOX sample showed the presence of γ-muurolene (17.78%), δ-cadinene (12.23%), bicyclogermacrene (7.77%), and α-copaene (7.17%) as main compounds. EOX presented a strong antioxidant potential according to the DPPH, TBARS, and nitrite production tests. Additionally, pretreatment with EOX, in mice, also significantly produced (P < 0.05 or P < 0.001) antinociceptive effect by reduction of nociceptive behavior (in formalin and writhing tests). The EOX showed c-Fos label in the olfactory bulb, piriform cortex, and periaqueductal gray. Acute administration of EOX exhibited a significant (P < 0.01 or P < 0.001) anti-inflammatory profile in the carrageenan-induced peritonitis and by the carrageenan-induced hindpaw edema tests in mice. Our results provide evidence for the use of X. laevigata by traditional medicine practitioners in the management of pain and inflammatory disorders.

Show MeSH

Related in: MedlinePlus

Immunofluorescence staining of nuclear c-Fos in the neurons of the olfactory bulb (a, d, g, and j), piriform cortex (b, e, h, and k), and periaqueductal gray (c, f, i, and l) 90 minutes after X. laevigata leaf essential oil (EOX) intraperitoneal injection at doses of 0 (saline control), 12.5, 25, and 50 mg/Kg, respectively, on these three brain regions of the animals submitted to carrageenan hypernociception model.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4109226&req=5

fig3: Immunofluorescence staining of nuclear c-Fos in the neurons of the olfactory bulb (a, d, g, and j), piriform cortex (b, e, h, and k), and periaqueductal gray (c, f, i, and l) 90 minutes after X. laevigata leaf essential oil (EOX) intraperitoneal injection at doses of 0 (saline control), 12.5, 25, and 50 mg/Kg, respectively, on these three brain regions of the animals submitted to carrageenan hypernociception model.

Mentions: In the olfactory bulb and in the piriform cortex (Figure 3 and Table 5) of the animals, the average number of neurons showing c-Fos was increased by an intraperitoneal injection of EOX at doses of 25 and 50 mg/Kg when compared with control (vehicle). However, the intraperitoneal injections of EOX at dose of 12.5 mg/Kg did not change the average number of neurons showing c-Fos when compared with control (vehicle).


Evaluation of the anti-inflammatory and antinociceptive effects of the essential oil from leaves of Xylopia laevigata in experimental models.

Queiroz JC, Antoniolli AR, Quintans-Júnior LJ, Brito RG, Barreto RS, Costa EV, da Silva TB, Prata AP, de Lucca W, Almeida JR, Lima JT, Quintans JS - ScientificWorldJournal (2014)

Immunofluorescence staining of nuclear c-Fos in the neurons of the olfactory bulb (a, d, g, and j), piriform cortex (b, e, h, and k), and periaqueductal gray (c, f, i, and l) 90 minutes after X. laevigata leaf essential oil (EOX) intraperitoneal injection at doses of 0 (saline control), 12.5, 25, and 50 mg/Kg, respectively, on these three brain regions of the animals submitted to carrageenan hypernociception model.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4109226&req=5

fig3: Immunofluorescence staining of nuclear c-Fos in the neurons of the olfactory bulb (a, d, g, and j), piriform cortex (b, e, h, and k), and periaqueductal gray (c, f, i, and l) 90 minutes after X. laevigata leaf essential oil (EOX) intraperitoneal injection at doses of 0 (saline control), 12.5, 25, and 50 mg/Kg, respectively, on these three brain regions of the animals submitted to carrageenan hypernociception model.
Mentions: In the olfactory bulb and in the piriform cortex (Figure 3 and Table 5) of the animals, the average number of neurons showing c-Fos was increased by an intraperitoneal injection of EOX at doses of 25 and 50 mg/Kg when compared with control (vehicle). However, the intraperitoneal injections of EOX at dose of 12.5 mg/Kg did not change the average number of neurons showing c-Fos when compared with control (vehicle).

Bottom Line: Thus, we investigated the possible antioxidant, antinociceptive, and anti-inflammatory effects of X. laevigata leaf essential oil (EOX) in animal models.Additionally, pretreatment with EOX, in mice, also significantly produced (P < 0.05 or P < 0.001) antinociceptive effect by reduction of nociceptive behavior (in formalin and writhing tests).The EOX showed c-Fos label in the olfactory bulb, piriform cortex, and periaqueductal gray.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Fisiologia, Universidade Federal de Sergipe, 49.000-100 São Cristóvão, SE, Brazil.

ABSTRACT
Xylopia laevigata (Annonaceae) is a medicinal plant used in folk medicine to treat pain and inflammation. Thus, we investigated the possible antioxidant, antinociceptive, and anti-inflammatory effects of X. laevigata leaf essential oil (EOX) in animal models. Our EOX sample showed the presence of γ-muurolene (17.78%), δ-cadinene (12.23%), bicyclogermacrene (7.77%), and α-copaene (7.17%) as main compounds. EOX presented a strong antioxidant potential according to the DPPH, TBARS, and nitrite production tests. Additionally, pretreatment with EOX, in mice, also significantly produced (P < 0.05 or P < 0.001) antinociceptive effect by reduction of nociceptive behavior (in formalin and writhing tests). The EOX showed c-Fos label in the olfactory bulb, piriform cortex, and periaqueductal gray. Acute administration of EOX exhibited a significant (P < 0.01 or P < 0.001) anti-inflammatory profile in the carrageenan-induced peritonitis and by the carrageenan-induced hindpaw edema tests in mice. Our results provide evidence for the use of X. laevigata by traditional medicine practitioners in the management of pain and inflammatory disorders.

Show MeSH
Related in: MedlinePlus