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Evaluation of the anti-inflammatory and antinociceptive effects of the essential oil from leaves of Xylopia laevigata in experimental models.

Queiroz JC, Antoniolli AR, Quintans-Júnior LJ, Brito RG, Barreto RS, Costa EV, da Silva TB, Prata AP, de Lucca W, Almeida JR, Lima JT, Quintans JS - ScientificWorldJournal (2014)

Bottom Line: Thus, we investigated the possible antioxidant, antinociceptive, and anti-inflammatory effects of X. laevigata leaf essential oil (EOX) in animal models.Additionally, pretreatment with EOX, in mice, also significantly produced (P < 0.05 or P < 0.001) antinociceptive effect by reduction of nociceptive behavior (in formalin and writhing tests).The EOX showed c-Fos label in the olfactory bulb, piriform cortex, and periaqueductal gray.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Fisiologia, Universidade Federal de Sergipe, 49.000-100 São Cristóvão, SE, Brazil.

ABSTRACT
Xylopia laevigata (Annonaceae) is a medicinal plant used in folk medicine to treat pain and inflammation. Thus, we investigated the possible antioxidant, antinociceptive, and anti-inflammatory effects of X. laevigata leaf essential oil (EOX) in animal models. Our EOX sample showed the presence of γ-muurolene (17.78%), δ-cadinene (12.23%), bicyclogermacrene (7.77%), and α-copaene (7.17%) as main compounds. EOX presented a strong antioxidant potential according to the DPPH, TBARS, and nitrite production tests. Additionally, pretreatment with EOX, in mice, also significantly produced (P < 0.05 or P < 0.001) antinociceptive effect by reduction of nociceptive behavior (in formalin and writhing tests). The EOX showed c-Fos label in the olfactory bulb, piriform cortex, and periaqueductal gray. Acute administration of EOX exhibited a significant (P < 0.01 or P < 0.001) anti-inflammatory profile in the carrageenan-induced peritonitis and by the carrageenan-induced hindpaw edema tests in mice. Our results provide evidence for the use of X. laevigata by traditional medicine practitioners in the management of pain and inflammatory disorders.

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NO-scavenging activity. Control means basal NO production of vehicle (DMSO 10%) in the absence of a NO generator source (without SNP); SNP group reflects nitrite production by sodium nitroprusside alone, considered 100% of NO production. The effect of different concentrations of EOX against SNP was determined by the Griess method. Values represent mean ± S.E.M., n = 4, experiments in triplicate. #P < 0.001 versus control;  *P < 0.01 different versus SNP (ANOVA followed by Tukey's test).
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fig2: NO-scavenging activity. Control means basal NO production of vehicle (DMSO 10%) in the absence of a NO generator source (without SNP); SNP group reflects nitrite production by sodium nitroprusside alone, considered 100% of NO production. The effect of different concentrations of EOX against SNP was determined by the Griess method. Values represent mean ± S.E.M., n = 4, experiments in triplicate. #P < 0.001 versus control;  *P < 0.01 different versus SNP (ANOVA followed by Tukey's test).

Mentions: To determine the ability of EOX to act as a reactive nitrogen species (RNS) scavenger, we evaluated the NO-scavenging activity by incubating EOX with sodium nitroprusside (SNP), a chemical inducer of NO production. EOX at 10 μg/mL, 100 μg/mL, and 1 mg/mL showed a significant NO-scavenging activity with P < 0.01 (Figure 2).


Evaluation of the anti-inflammatory and antinociceptive effects of the essential oil from leaves of Xylopia laevigata in experimental models.

Queiroz JC, Antoniolli AR, Quintans-Júnior LJ, Brito RG, Barreto RS, Costa EV, da Silva TB, Prata AP, de Lucca W, Almeida JR, Lima JT, Quintans JS - ScientificWorldJournal (2014)

NO-scavenging activity. Control means basal NO production of vehicle (DMSO 10%) in the absence of a NO generator source (without SNP); SNP group reflects nitrite production by sodium nitroprusside alone, considered 100% of NO production. The effect of different concentrations of EOX against SNP was determined by the Griess method. Values represent mean ± S.E.M., n = 4, experiments in triplicate. #P < 0.001 versus control;  *P < 0.01 different versus SNP (ANOVA followed by Tukey's test).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4109226&req=5

fig2: NO-scavenging activity. Control means basal NO production of vehicle (DMSO 10%) in the absence of a NO generator source (without SNP); SNP group reflects nitrite production by sodium nitroprusside alone, considered 100% of NO production. The effect of different concentrations of EOX against SNP was determined by the Griess method. Values represent mean ± S.E.M., n = 4, experiments in triplicate. #P < 0.001 versus control;  *P < 0.01 different versus SNP (ANOVA followed by Tukey's test).
Mentions: To determine the ability of EOX to act as a reactive nitrogen species (RNS) scavenger, we evaluated the NO-scavenging activity by incubating EOX with sodium nitroprusside (SNP), a chemical inducer of NO production. EOX at 10 μg/mL, 100 μg/mL, and 1 mg/mL showed a significant NO-scavenging activity with P < 0.01 (Figure 2).

Bottom Line: Thus, we investigated the possible antioxidant, antinociceptive, and anti-inflammatory effects of X. laevigata leaf essential oil (EOX) in animal models.Additionally, pretreatment with EOX, in mice, also significantly produced (P < 0.05 or P < 0.001) antinociceptive effect by reduction of nociceptive behavior (in formalin and writhing tests).The EOX showed c-Fos label in the olfactory bulb, piriform cortex, and periaqueductal gray.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Fisiologia, Universidade Federal de Sergipe, 49.000-100 São Cristóvão, SE, Brazil.

ABSTRACT
Xylopia laevigata (Annonaceae) is a medicinal plant used in folk medicine to treat pain and inflammation. Thus, we investigated the possible antioxidant, antinociceptive, and anti-inflammatory effects of X. laevigata leaf essential oil (EOX) in animal models. Our EOX sample showed the presence of γ-muurolene (17.78%), δ-cadinene (12.23%), bicyclogermacrene (7.77%), and α-copaene (7.17%) as main compounds. EOX presented a strong antioxidant potential according to the DPPH, TBARS, and nitrite production tests. Additionally, pretreatment with EOX, in mice, also significantly produced (P < 0.05 or P < 0.001) antinociceptive effect by reduction of nociceptive behavior (in formalin and writhing tests). The EOX showed c-Fos label in the olfactory bulb, piriform cortex, and periaqueductal gray. Acute administration of EOX exhibited a significant (P < 0.01 or P < 0.001) anti-inflammatory profile in the carrageenan-induced peritonitis and by the carrageenan-induced hindpaw edema tests in mice. Our results provide evidence for the use of X. laevigata by traditional medicine practitioners in the management of pain and inflammatory disorders.

Show MeSH
Related in: MedlinePlus