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Evaluation of the anti-inflammatory and antinociceptive effects of the essential oil from leaves of Xylopia laevigata in experimental models.

Queiroz JC, Antoniolli AR, Quintans-Júnior LJ, Brito RG, Barreto RS, Costa EV, da Silva TB, Prata AP, de Lucca W, Almeida JR, Lima JT, Quintans JS - ScientificWorldJournal (2014)

Bottom Line: Thus, we investigated the possible antioxidant, antinociceptive, and anti-inflammatory effects of X. laevigata leaf essential oil (EOX) in animal models.Additionally, pretreatment with EOX, in mice, also significantly produced (P < 0.05 or P < 0.001) antinociceptive effect by reduction of nociceptive behavior (in formalin and writhing tests).The EOX showed c-Fos label in the olfactory bulb, piriform cortex, and periaqueductal gray.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Fisiologia, Universidade Federal de Sergipe, 49.000-100 São Cristóvão, SE, Brazil.

ABSTRACT
Xylopia laevigata (Annonaceae) is a medicinal plant used in folk medicine to treat pain and inflammation. Thus, we investigated the possible antioxidant, antinociceptive, and anti-inflammatory effects of X. laevigata leaf essential oil (EOX) in animal models. Our EOX sample showed the presence of γ-muurolene (17.78%), δ-cadinene (12.23%), bicyclogermacrene (7.77%), and α-copaene (7.17%) as main compounds. EOX presented a strong antioxidant potential according to the DPPH, TBARS, and nitrite production tests. Additionally, pretreatment with EOX, in mice, also significantly produced (P < 0.05 or P < 0.001) antinociceptive effect by reduction of nociceptive behavior (in formalin and writhing tests). The EOX showed c-Fos label in the olfactory bulb, piriform cortex, and periaqueductal gray. Acute administration of EOX exhibited a significant (P < 0.01 or P < 0.001) anti-inflammatory profile in the carrageenan-induced peritonitis and by the carrageenan-induced hindpaw edema tests in mice. Our results provide evidence for the use of X. laevigata by traditional medicine practitioners in the management of pain and inflammatory disorders.

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TBARS in vitro. Lipid extracted from egg yolk was subjected to oxidative damage by incubation with AAPH, and the ability of different concentrations of EOX to prevent TBARS formation was analyzed. Control means basal lipid peroxidation with vehicle alone (DMSO 10%); AAPH alone group is considered as 100% of oxidative damage. Values represent mean ± S.E.M., n = 4, experiments in triplicate. #P < 0.001versus control;  *P < 0.05 and  **P < 0.001 different versus AAPH group (system) (ANOVA followed by Tukey's test).
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fig1: TBARS in vitro. Lipid extracted from egg yolk was subjected to oxidative damage by incubation with AAPH, and the ability of different concentrations of EOX to prevent TBARS formation was analyzed. Control means basal lipid peroxidation with vehicle alone (DMSO 10%); AAPH alone group is considered as 100% of oxidative damage. Values represent mean ± S.E.M., n = 4, experiments in triplicate. #P < 0.001versus control;  *P < 0.05 and  **P < 0.001 different versus AAPH group (system) (ANOVA followed by Tukey's test).

Mentions: To assess the antioxidant potential of EOX, we tested its ability to prevent oxidative damage to lipids induced by a free radical source in vitro (AAPH). Quantification by TBARS demonstrated that EOX exerts a significant (P < 0.05 or P < 0.001) antioxidant effect against peroxyl radicals generated by AAPH, protecting lipids from oxidation in a dose-dependent fashion (Figure 1).


Evaluation of the anti-inflammatory and antinociceptive effects of the essential oil from leaves of Xylopia laevigata in experimental models.

Queiroz JC, Antoniolli AR, Quintans-Júnior LJ, Brito RG, Barreto RS, Costa EV, da Silva TB, Prata AP, de Lucca W, Almeida JR, Lima JT, Quintans JS - ScientificWorldJournal (2014)

TBARS in vitro. Lipid extracted from egg yolk was subjected to oxidative damage by incubation with AAPH, and the ability of different concentrations of EOX to prevent TBARS formation was analyzed. Control means basal lipid peroxidation with vehicle alone (DMSO 10%); AAPH alone group is considered as 100% of oxidative damage. Values represent mean ± S.E.M., n = 4, experiments in triplicate. #P < 0.001versus control;  *P < 0.05 and  **P < 0.001 different versus AAPH group (system) (ANOVA followed by Tukey's test).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4109226&req=5

fig1: TBARS in vitro. Lipid extracted from egg yolk was subjected to oxidative damage by incubation with AAPH, and the ability of different concentrations of EOX to prevent TBARS formation was analyzed. Control means basal lipid peroxidation with vehicle alone (DMSO 10%); AAPH alone group is considered as 100% of oxidative damage. Values represent mean ± S.E.M., n = 4, experiments in triplicate. #P < 0.001versus control;  *P < 0.05 and  **P < 0.001 different versus AAPH group (system) (ANOVA followed by Tukey's test).
Mentions: To assess the antioxidant potential of EOX, we tested its ability to prevent oxidative damage to lipids induced by a free radical source in vitro (AAPH). Quantification by TBARS demonstrated that EOX exerts a significant (P < 0.05 or P < 0.001) antioxidant effect against peroxyl radicals generated by AAPH, protecting lipids from oxidation in a dose-dependent fashion (Figure 1).

Bottom Line: Thus, we investigated the possible antioxidant, antinociceptive, and anti-inflammatory effects of X. laevigata leaf essential oil (EOX) in animal models.Additionally, pretreatment with EOX, in mice, also significantly produced (P < 0.05 or P < 0.001) antinociceptive effect by reduction of nociceptive behavior (in formalin and writhing tests).The EOX showed c-Fos label in the olfactory bulb, piriform cortex, and periaqueductal gray.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Fisiologia, Universidade Federal de Sergipe, 49.000-100 São Cristóvão, SE, Brazil.

ABSTRACT
Xylopia laevigata (Annonaceae) is a medicinal plant used in folk medicine to treat pain and inflammation. Thus, we investigated the possible antioxidant, antinociceptive, and anti-inflammatory effects of X. laevigata leaf essential oil (EOX) in animal models. Our EOX sample showed the presence of γ-muurolene (17.78%), δ-cadinene (12.23%), bicyclogermacrene (7.77%), and α-copaene (7.17%) as main compounds. EOX presented a strong antioxidant potential according to the DPPH, TBARS, and nitrite production tests. Additionally, pretreatment with EOX, in mice, also significantly produced (P < 0.05 or P < 0.001) antinociceptive effect by reduction of nociceptive behavior (in formalin and writhing tests). The EOX showed c-Fos label in the olfactory bulb, piriform cortex, and periaqueductal gray. Acute administration of EOX exhibited a significant (P < 0.01 or P < 0.001) anti-inflammatory profile in the carrageenan-induced peritonitis and by the carrageenan-induced hindpaw edema tests in mice. Our results provide evidence for the use of X. laevigata by traditional medicine practitioners in the management of pain and inflammatory disorders.

Show MeSH
Related in: MedlinePlus