Limits...
Synergistic microbial consortium for bioenergy generation from complex natural energy sources.

Wang VB, Yam JK, Chua SL, Zhang Q, Cao B, Chye JL, Yang L - ScientificWorldJournal (2014)

Bottom Line: The monospecies S. oneidensis system was able to generate bioenergy in a short experimental time frame while the monospecies E. coli system generated significantly less bioenergy.The synergistic effect is suggested to arise from E. coli and S. oneidensis utilizing different nutrients as electron donors and effect of flavins secreted by S. oneidensis.Confocal images confirmed the presence of biofilms and point towards their importance in generating bioenergy in MFCs.

View Article: PubMed Central - PubMed

Affiliation: Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore 637551 ; School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798.

ABSTRACT
Microbial species have evolved diverse mechanisms for utilization of complex carbon sources. Proper combination of targeted species can affect bioenergy production from natural waste products. Here, we established a stable microbial consortium with Escherichia coli and Shewanella oneidensis in microbial fuel cells (MFCs) to produce bioenergy from an abundant natural energy source, in the form of the sarcocarp harvested from coconuts. This component is mostly discarded as waste. However, through its usage as a feedstock for MFCs to produce useful energy in this study, the sarcocarp can be utilized meaningfully. The monospecies S. oneidensis system was able to generate bioenergy in a short experimental time frame while the monospecies E. coli system generated significantly less bioenergy. A combination of E. coli and S. oneidensis in the ratio of 1:9 (v:v) significantly enhanced the experimental time frame and magnitude of bioenergy generation. The synergistic effect is suggested to arise from E. coli and S. oneidensis utilizing different nutrients as electron donors and effect of flavins secreted by S. oneidensis. Confocal images confirmed the presence of biofilms and point towards their importance in generating bioenergy in MFCs.

Show MeSH

Related in: MedlinePlus

Overlaid brightfield and confocal microscopy images of stained biofilms on respective electrodes. (a) E. coli biofilm. (b) S. oneidensis biofilm.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4109225&req=5

fig2: Overlaid brightfield and confocal microscopy images of stained biofilms on respective electrodes. (a) E. coli biofilm. (b) S. oneidensis biofilm.

Mentions: Further, the role of biofilms in the bioelectrochemical systems was elucidated by confocal microscopy characterization. Representative overlaid brightfield and confocal images were acquired from random strands of electrodes in respective MFCs. Biofilms were formed in all systems (Figures 2(a) and 2(b)). To differentiate between each species, E. coli was tagged with red fluorescent protein (RFP), whereas S. oneidensis was tagged with green fluorescent protein (GFP). The RFP-tagged E. coli biofilm and GFP-tagged S. oneidensis biofilm were evident on the electrode fiber surfaces (Figures 2(a) and 2(b)). The confocal images corroborate the importance of the biofilm in the electrical performances with specific bacterial strains.


Synergistic microbial consortium for bioenergy generation from complex natural energy sources.

Wang VB, Yam JK, Chua SL, Zhang Q, Cao B, Chye JL, Yang L - ScientificWorldJournal (2014)

Overlaid brightfield and confocal microscopy images of stained biofilms on respective electrodes. (a) E. coli biofilm. (b) S. oneidensis biofilm.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4109225&req=5

fig2: Overlaid brightfield and confocal microscopy images of stained biofilms on respective electrodes. (a) E. coli biofilm. (b) S. oneidensis biofilm.
Mentions: Further, the role of biofilms in the bioelectrochemical systems was elucidated by confocal microscopy characterization. Representative overlaid brightfield and confocal images were acquired from random strands of electrodes in respective MFCs. Biofilms were formed in all systems (Figures 2(a) and 2(b)). To differentiate between each species, E. coli was tagged with red fluorescent protein (RFP), whereas S. oneidensis was tagged with green fluorescent protein (GFP). The RFP-tagged E. coli biofilm and GFP-tagged S. oneidensis biofilm were evident on the electrode fiber surfaces (Figures 2(a) and 2(b)). The confocal images corroborate the importance of the biofilm in the electrical performances with specific bacterial strains.

Bottom Line: The monospecies S. oneidensis system was able to generate bioenergy in a short experimental time frame while the monospecies E. coli system generated significantly less bioenergy.The synergistic effect is suggested to arise from E. coli and S. oneidensis utilizing different nutrients as electron donors and effect of flavins secreted by S. oneidensis.Confocal images confirmed the presence of biofilms and point towards their importance in generating bioenergy in MFCs.

View Article: PubMed Central - PubMed

Affiliation: Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore 637551 ; School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798.

ABSTRACT
Microbial species have evolved diverse mechanisms for utilization of complex carbon sources. Proper combination of targeted species can affect bioenergy production from natural waste products. Here, we established a stable microbial consortium with Escherichia coli and Shewanella oneidensis in microbial fuel cells (MFCs) to produce bioenergy from an abundant natural energy source, in the form of the sarcocarp harvested from coconuts. This component is mostly discarded as waste. However, through its usage as a feedstock for MFCs to produce useful energy in this study, the sarcocarp can be utilized meaningfully. The monospecies S. oneidensis system was able to generate bioenergy in a short experimental time frame while the monospecies E. coli system generated significantly less bioenergy. A combination of E. coli and S. oneidensis in the ratio of 1:9 (v:v) significantly enhanced the experimental time frame and magnitude of bioenergy generation. The synergistic effect is suggested to arise from E. coli and S. oneidensis utilizing different nutrients as electron donors and effect of flavins secreted by S. oneidensis. Confocal images confirmed the presence of biofilms and point towards their importance in generating bioenergy in MFCs.

Show MeSH
Related in: MedlinePlus