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Effect of Pseudomonas putida on growth and anthocyanin pigment in two poinsettia (Euphorbia pulcherrima) cultivars.

Zulueta-Rodriguez R, Cordoba-Matson MV, Hernandez-Montiel LG, Murillo-Amador B, Rueda-Puente E, Lara L - ScientificWorldJournal (2014)

Bottom Line: For all plants with P. putida strains, there was less anthocyanin pigment, but biomass was always greater with PGPR strains.Nevertheless, to the naked eye, the coloration of the plants appeared identical in color compared to the control group.This is the first study reporting the positive effects of P. putida rhizobacteria treatments on growth of poinsettia cultivars.

View Article: PubMed Central - PubMed

Affiliation: Facultad de Ciencias Agricolas, Universidad Veracruzana, Circuito Universitario Gonzalo Aguirre Beltran S/N, Zona Universitaria, 91090 Xalapa, VER, Mexico.

ABSTRACT
Pseudomonas putida is plant growth promoting rhizobacteria (PGPR) that have the capacity to improve growth in plants. The purpose of this study was to determine growth and anthocyanin pigmentation of the bracts in two poinsettia Euphorbia pulcherrima cultivars (Prestige and Sonora Marble) using three strains of P. putida, as well as a mixture of the three (MIX). Comparison with the control group indicated for the most part that Prestige grew better than the Sonora Marble cultivars with the PGPR strains. Prestige with the MIX strain grew better compared to control for the number of cyathia (83 versus 70.4), volume of roots (45 versus 3  cm(3)), number of leaves (78 versus 58), and area of leaf (1,788 versus 1,331 cm(2)), except for the number of flowers (8.8 versus 11.6). To the naked eye, coloration of plants appeared identical in color compared to the control group. For all plants with P. putida strains, there was less anthocyanin pigment, but biomass was always greater with PGPR strains. Nevertheless, to the naked eye, the coloration of the plants appeared identical in color compared to the control group. This is the first study reporting the positive effects of P. putida rhizobacteria treatments on growth of poinsettia cultivars.

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Related in: MedlinePlus

Population of Pseudomonas putida strains in the rhizosphere of poinsettia Prestige and Sonora Marble cultivars. Colony forming units (CFU) in plants with P. putida strain: FCA-8, FCA-56, FCA-60, and MIX (inoculated simultaneously with the three rhizobacteria) at 110 DAI. Data for controls of both Prestige and Sonora Marble cultivars are not shown since they were found to have 0 CFU.
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fig1: Population of Pseudomonas putida strains in the rhizosphere of poinsettia Prestige and Sonora Marble cultivars. Colony forming units (CFU) in plants with P. putida strain: FCA-8, FCA-56, FCA-60, and MIX (inoculated simultaneously with the three rhizobacteria) at 110 DAI. Data for controls of both Prestige and Sonora Marble cultivars are not shown since they were found to have 0 CFU.

Mentions: Even though the strains of P. putida have a positive effect on plant growth in our experiment, Sonora Marble did not show a positive response in most of its other variables. This effect is possibly due to low P. putida colonization in the root of this cultivar (Figure 1), which was less compared to the other cultivars. For both Prestige and Sonora Marble cultivars, control groups without P. putida had 0 CFU colonization, indicating that there was no contamination with other bacteria (data not shown). One possibility for this behavior is that the Sonora Marble possibly exudates compounds in its roots which limit colonization of different strains of P. putida [29, 30]. In plants such as tomato, the colonization of the roots for rhizobacteria depends on a great variety of chemical compounds such as succinic acid, malic acid, L-aspartic, L-glutamic acid, L-isoleucine, L-leucine, and L-lysine [31]. It has been reported that Prestige exudates compounds in the root which are favorable for colonization [32]. Hence, in Prestige the establishment of its rhizosphere was probably aided by root exudates that favored P. putida colonization [33, 34]. Nevertheless, literature searches revealed no information about the type or amount of compounds in poinsettias exudates with rhizobacteria.


Effect of Pseudomonas putida on growth and anthocyanin pigment in two poinsettia (Euphorbia pulcherrima) cultivars.

Zulueta-Rodriguez R, Cordoba-Matson MV, Hernandez-Montiel LG, Murillo-Amador B, Rueda-Puente E, Lara L - ScientificWorldJournal (2014)

Population of Pseudomonas putida strains in the rhizosphere of poinsettia Prestige and Sonora Marble cultivars. Colony forming units (CFU) in plants with P. putida strain: FCA-8, FCA-56, FCA-60, and MIX (inoculated simultaneously with the three rhizobacteria) at 110 DAI. Data for controls of both Prestige and Sonora Marble cultivars are not shown since they were found to have 0 CFU.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4109214&req=5

fig1: Population of Pseudomonas putida strains in the rhizosphere of poinsettia Prestige and Sonora Marble cultivars. Colony forming units (CFU) in plants with P. putida strain: FCA-8, FCA-56, FCA-60, and MIX (inoculated simultaneously with the three rhizobacteria) at 110 DAI. Data for controls of both Prestige and Sonora Marble cultivars are not shown since they were found to have 0 CFU.
Mentions: Even though the strains of P. putida have a positive effect on plant growth in our experiment, Sonora Marble did not show a positive response in most of its other variables. This effect is possibly due to low P. putida colonization in the root of this cultivar (Figure 1), which was less compared to the other cultivars. For both Prestige and Sonora Marble cultivars, control groups without P. putida had 0 CFU colonization, indicating that there was no contamination with other bacteria (data not shown). One possibility for this behavior is that the Sonora Marble possibly exudates compounds in its roots which limit colonization of different strains of P. putida [29, 30]. In plants such as tomato, the colonization of the roots for rhizobacteria depends on a great variety of chemical compounds such as succinic acid, malic acid, L-aspartic, L-glutamic acid, L-isoleucine, L-leucine, and L-lysine [31]. It has been reported that Prestige exudates compounds in the root which are favorable for colonization [32]. Hence, in Prestige the establishment of its rhizosphere was probably aided by root exudates that favored P. putida colonization [33, 34]. Nevertheless, literature searches revealed no information about the type or amount of compounds in poinsettias exudates with rhizobacteria.

Bottom Line: For all plants with P. putida strains, there was less anthocyanin pigment, but biomass was always greater with PGPR strains.Nevertheless, to the naked eye, the coloration of the plants appeared identical in color compared to the control group.This is the first study reporting the positive effects of P. putida rhizobacteria treatments on growth of poinsettia cultivars.

View Article: PubMed Central - PubMed

Affiliation: Facultad de Ciencias Agricolas, Universidad Veracruzana, Circuito Universitario Gonzalo Aguirre Beltran S/N, Zona Universitaria, 91090 Xalapa, VER, Mexico.

ABSTRACT
Pseudomonas putida is plant growth promoting rhizobacteria (PGPR) that have the capacity to improve growth in plants. The purpose of this study was to determine growth and anthocyanin pigmentation of the bracts in two poinsettia Euphorbia pulcherrima cultivars (Prestige and Sonora Marble) using three strains of P. putida, as well as a mixture of the three (MIX). Comparison with the control group indicated for the most part that Prestige grew better than the Sonora Marble cultivars with the PGPR strains. Prestige with the MIX strain grew better compared to control for the number of cyathia (83 versus 70.4), volume of roots (45 versus 3  cm(3)), number of leaves (78 versus 58), and area of leaf (1,788 versus 1,331 cm(2)), except for the number of flowers (8.8 versus 11.6). To the naked eye, coloration of plants appeared identical in color compared to the control group. For all plants with P. putida strains, there was less anthocyanin pigment, but biomass was always greater with PGPR strains. Nevertheless, to the naked eye, the coloration of the plants appeared identical in color compared to the control group. This is the first study reporting the positive effects of P. putida rhizobacteria treatments on growth of poinsettia cultivars.

Show MeSH
Related in: MedlinePlus