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Reliability of a rapid hematology stain for sputum cytology.

Gonçalves J, Pizzichini E, Pizzichini MM, Steidle LJ, Rocha CC, Ferreira SC, Zimmermann CT - J Bras Pneumol (2014 May-Jun)

Bottom Line: In our comparison of the two staining techniques, the ICCs indicated almost perfect interobserver agreement for neutrophil, eosinophil, and macrophage counts (ICC: 0.98-1.00), as well as substantial agreement for lymphocyte counts (ICC: 0.76-0.83).Interobserver agreement for the identification of eosinophilic and neutrophilic sputum using the two techniques ranged from substantial to almost perfect (kappa range: 0.91-1.00).The use of Diff-Quik can be considered a reliable alternative for the processing of sputum samples.

View Article: PubMed Central - PubMed

Affiliation: Clinical Analysis Department, University Hospital, Federal University of Santa Catarina, Florianópolis, Brazil.

ABSTRACT

Objective: To determine the reliability of a rapid hematology stain for the cytological analysis of induced sputum samples.

Methods: This was a cross-sectional study comparing the standard technique (May-Grünwald-Giemsa stain) with a rapid hematology stain (Diff-Quik). Of the 50 subjects included in the study, 21 had asthma, 19 had COPD, and 10 were healthy (controls). From the induced sputum samples collected, we prepared four slides: two were stained with May-Grünwald-Giemsa, and two were stained with Diff-Quik. The slides were read independently by two trained researchers blinded to the identification of the slides. The reliability for cell counting using the two techniques was evaluated by determining the intraclass correlation coefficients (ICCs) for intraobserver and interobserver agreement. Agreement in the identification of neutrophilic and eosinophilic sputum between the observers and between the stains was evaluated with kappa statistics.

Results: In our comparison of the two staining techniques, the ICCs indicated almost perfect interobserver agreement for neutrophil, eosinophil, and macrophage counts (ICC: 0.98-1.00), as well as substantial agreement for lymphocyte counts (ICC: 0.76-0.83). Intraobserver agreement was almost perfect for neutrophil, eosinophil, and macrophage counts (ICC: 0.96-0.99), whereas it was moderate to substantial for lymphocyte counts (ICC = 0.65 and 0.75 for the two observers, respectively). Interobserver agreement for the identification of eosinophilic and neutrophilic sputum using the two techniques ranged from substantial to almost perfect (kappa range: 0.91-1.00).

Conclusions: The use of Diff-Quik can be considered a reliable alternative for the processing of sputum samples.

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Related in: MedlinePlus

Celularidade diferencial no escarro induzido nos três grupos doestudo.
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Related In: Results  -  Collection

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f04: Celularidade diferencial no escarro induzido nos três grupos doestudo.

Mentions: As características celulares do escarro induzido foram as esperadas para osdiferentes grupos do estudo. Quando comparados com portadores de DPOC e controlessaudáveis, o escarro dos asmáticos caracterizou-se por uma proporçãosignificativamente maior de eosinófilos. Em contraste, o escarro dos portadores deDPOC apresentou um aumento significativo na contagem celular total e na proporção deneutrófilos do que o do grupo controle. O grupo controle apresentou uma proporçãosignificativamente maior de macrófagos que os outros dois grupos. A Figura 1 mostra as proporções de neutrófilos,eosinófilos e macrófagos nos diferentes grupos do estudo. Na Figura 2, as medianas das proporções de neutrófilos, eosinófilose macrófagos, em toda a amostra estudada, estão separadas pelo tipo de coloraçãoempregada. Observa-se que não houve diferenças entre as contagens das lâminas coradaspelos dois métodos utilizados.


Reliability of a rapid hematology stain for sputum cytology.

Gonçalves J, Pizzichini E, Pizzichini MM, Steidle LJ, Rocha CC, Ferreira SC, Zimmermann CT - J Bras Pneumol (2014 May-Jun)

Celularidade diferencial no escarro induzido nos três grupos doestudo.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109197&req=5

f04: Celularidade diferencial no escarro induzido nos três grupos doestudo.
Mentions: As características celulares do escarro induzido foram as esperadas para osdiferentes grupos do estudo. Quando comparados com portadores de DPOC e controlessaudáveis, o escarro dos asmáticos caracterizou-se por uma proporçãosignificativamente maior de eosinófilos. Em contraste, o escarro dos portadores deDPOC apresentou um aumento significativo na contagem celular total e na proporção deneutrófilos do que o do grupo controle. O grupo controle apresentou uma proporçãosignificativamente maior de macrófagos que os outros dois grupos. A Figura 1 mostra as proporções de neutrófilos,eosinófilos e macrófagos nos diferentes grupos do estudo. Na Figura 2, as medianas das proporções de neutrófilos, eosinófilose macrófagos, em toda a amostra estudada, estão separadas pelo tipo de coloraçãoempregada. Observa-se que não houve diferenças entre as contagens das lâminas coradaspelos dois métodos utilizados.

Bottom Line: In our comparison of the two staining techniques, the ICCs indicated almost perfect interobserver agreement for neutrophil, eosinophil, and macrophage counts (ICC: 0.98-1.00), as well as substantial agreement for lymphocyte counts (ICC: 0.76-0.83).Interobserver agreement for the identification of eosinophilic and neutrophilic sputum using the two techniques ranged from substantial to almost perfect (kappa range: 0.91-1.00).The use of Diff-Quik can be considered a reliable alternative for the processing of sputum samples.

View Article: PubMed Central - PubMed

Affiliation: Clinical Analysis Department, University Hospital, Federal University of Santa Catarina, Florianópolis, Brazil.

ABSTRACT

Objective: To determine the reliability of a rapid hematology stain for the cytological analysis of induced sputum samples.

Methods: This was a cross-sectional study comparing the standard technique (May-Grünwald-Giemsa stain) with a rapid hematology stain (Diff-Quik). Of the 50 subjects included in the study, 21 had asthma, 19 had COPD, and 10 were healthy (controls). From the induced sputum samples collected, we prepared four slides: two were stained with May-Grünwald-Giemsa, and two were stained with Diff-Quik. The slides were read independently by two trained researchers blinded to the identification of the slides. The reliability for cell counting using the two techniques was evaluated by determining the intraclass correlation coefficients (ICCs) for intraobserver and interobserver agreement. Agreement in the identification of neutrophilic and eosinophilic sputum between the observers and between the stains was evaluated with kappa statistics.

Results: In our comparison of the two staining techniques, the ICCs indicated almost perfect interobserver agreement for neutrophil, eosinophil, and macrophage counts (ICC: 0.98-1.00), as well as substantial agreement for lymphocyte counts (ICC: 0.76-0.83). Intraobserver agreement was almost perfect for neutrophil, eosinophil, and macrophage counts (ICC: 0.96-0.99), whereas it was moderate to substantial for lymphocyte counts (ICC = 0.65 and 0.75 for the two observers, respectively). Interobserver agreement for the identification of eosinophilic and neutrophilic sputum using the two techniques ranged from substantial to almost perfect (kappa range: 0.91-1.00).

Conclusions: The use of Diff-Quik can be considered a reliable alternative for the processing of sputum samples.

Show MeSH
Related in: MedlinePlus