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Reliability of a rapid hematology stain for sputum cytology.

Gonçalves J, Pizzichini E, Pizzichini MM, Steidle LJ, Rocha CC, Ferreira SC, Zimmermann CT - J Bras Pneumol (2014 May-Jun)

Bottom Line: In our comparison of the two staining techniques, the ICCs indicated almost perfect interobserver agreement for neutrophil, eosinophil, and macrophage counts (ICC: 0.98-1.00), as well as substantial agreement for lymphocyte counts (ICC: 0.76-0.83).Interobserver agreement for the identification of eosinophilic and neutrophilic sputum using the two techniques ranged from substantial to almost perfect (kappa range: 0.91-1.00).The use of Diff-Quik can be considered a reliable alternative for the processing of sputum samples.

View Article: PubMed Central - PubMed

Affiliation: Clinical Analysis Department, University Hospital, Federal University of Santa Catarina, Florianópolis, Brazil.

ABSTRACT

Objective: To determine the reliability of a rapid hematology stain for the cytological analysis of induced sputum samples.

Methods: This was a cross-sectional study comparing the standard technique (May-Grünwald-Giemsa stain) with a rapid hematology stain (Diff-Quik). Of the 50 subjects included in the study, 21 had asthma, 19 had COPD, and 10 were healthy (controls). From the induced sputum samples collected, we prepared four slides: two were stained with May-Grünwald-Giemsa, and two were stained with Diff-Quik. The slides were read independently by two trained researchers blinded to the identification of the slides. The reliability for cell counting using the two techniques was evaluated by determining the intraclass correlation coefficients (ICCs) for intraobserver and interobserver agreement. Agreement in the identification of neutrophilic and eosinophilic sputum between the observers and between the stains was evaluated with kappa statistics.

Results: In our comparison of the two staining techniques, the ICCs indicated almost perfect interobserver agreement for neutrophil, eosinophil, and macrophage counts (ICC: 0.98-1.00), as well as substantial agreement for lymphocyte counts (ICC: 0.76-0.83). Intraobserver agreement was almost perfect for neutrophil, eosinophil, and macrophage counts (ICC: 0.96-0.99), whereas it was moderate to substantial for lymphocyte counts (ICC = 0.65 and 0.75 for the two observers, respectively). Interobserver agreement for the identification of eosinophilic and neutrophilic sputum using the two techniques ranged from substantial to almost perfect (kappa range: 0.91-1.00).

Conclusions: The use of Diff-Quik can be considered a reliable alternative for the processing of sputum samples.

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Related in: MedlinePlus

Induced sputum differential cell counts in the three groupsstudied.
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Related In: Results  -  Collection

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f01: Induced sputum differential cell counts in the three groupsstudied.

Mentions: The cellular characteristics of induced sputum were as expected for the different groupsstudied. The sputum of asthma patients was characterized by a significantly higherproportion of eosinophils than that found in the sputum of COPD patients and healthycontrols. In contrast, the sputum of COPD patients showed a significant increase intotal cell counts and in the proportion of neutrophils when compared with that ofcontrols. The control group showed a significantly higher proportion of macrophages thandid the other two groups. Figure 1 shows theproportions of neutrophils, eosinophils, and macrophages in the different groupsstudied. In Figure 2, the median proportions ofneutrophils, eosinophils, and macrophages, in the study sample as a whole, are separatedby type of stain used. No significant differences were found for the cell counts on theslides stained by either of the two techniques used.


Reliability of a rapid hematology stain for sputum cytology.

Gonçalves J, Pizzichini E, Pizzichini MM, Steidle LJ, Rocha CC, Ferreira SC, Zimmermann CT - J Bras Pneumol (2014 May-Jun)

Induced sputum differential cell counts in the three groupsstudied.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109197&req=5

f01: Induced sputum differential cell counts in the three groupsstudied.
Mentions: The cellular characteristics of induced sputum were as expected for the different groupsstudied. The sputum of asthma patients was characterized by a significantly higherproportion of eosinophils than that found in the sputum of COPD patients and healthycontrols. In contrast, the sputum of COPD patients showed a significant increase intotal cell counts and in the proportion of neutrophils when compared with that ofcontrols. The control group showed a significantly higher proportion of macrophages thandid the other two groups. Figure 1 shows theproportions of neutrophils, eosinophils, and macrophages in the different groupsstudied. In Figure 2, the median proportions ofneutrophils, eosinophils, and macrophages, in the study sample as a whole, are separatedby type of stain used. No significant differences were found for the cell counts on theslides stained by either of the two techniques used.

Bottom Line: In our comparison of the two staining techniques, the ICCs indicated almost perfect interobserver agreement for neutrophil, eosinophil, and macrophage counts (ICC: 0.98-1.00), as well as substantial agreement for lymphocyte counts (ICC: 0.76-0.83).Interobserver agreement for the identification of eosinophilic and neutrophilic sputum using the two techniques ranged from substantial to almost perfect (kappa range: 0.91-1.00).The use of Diff-Quik can be considered a reliable alternative for the processing of sputum samples.

View Article: PubMed Central - PubMed

Affiliation: Clinical Analysis Department, University Hospital, Federal University of Santa Catarina, Florianópolis, Brazil.

ABSTRACT

Objective: To determine the reliability of a rapid hematology stain for the cytological analysis of induced sputum samples.

Methods: This was a cross-sectional study comparing the standard technique (May-Grünwald-Giemsa stain) with a rapid hematology stain (Diff-Quik). Of the 50 subjects included in the study, 21 had asthma, 19 had COPD, and 10 were healthy (controls). From the induced sputum samples collected, we prepared four slides: two were stained with May-Grünwald-Giemsa, and two were stained with Diff-Quik. The slides were read independently by two trained researchers blinded to the identification of the slides. The reliability for cell counting using the two techniques was evaluated by determining the intraclass correlation coefficients (ICCs) for intraobserver and interobserver agreement. Agreement in the identification of neutrophilic and eosinophilic sputum between the observers and between the stains was evaluated with kappa statistics.

Results: In our comparison of the two staining techniques, the ICCs indicated almost perfect interobserver agreement for neutrophil, eosinophil, and macrophage counts (ICC: 0.98-1.00), as well as substantial agreement for lymphocyte counts (ICC: 0.76-0.83). Intraobserver agreement was almost perfect for neutrophil, eosinophil, and macrophage counts (ICC: 0.96-0.99), whereas it was moderate to substantial for lymphocyte counts (ICC = 0.65 and 0.75 for the two observers, respectively). Interobserver agreement for the identification of eosinophilic and neutrophilic sputum using the two techniques ranged from substantial to almost perfect (kappa range: 0.91-1.00).

Conclusions: The use of Diff-Quik can be considered a reliable alternative for the processing of sputum samples.

Show MeSH
Related in: MedlinePlus