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Effects of acute and chronic administration of methylprednisolone on oxidative stress in rat lungs.

Torres RL, Torres IL, Laste G, Ferreira MB, Cardoso PF, Belló-Klein A - J Bras Pneumol (2014 May-Jun)

Bottom Line: To determine the effects of acute and chronic administration of methylprednisolone on oxidative stress, as quantified by measuring lipid peroxidation (LPO) and total reactive antioxidant potential (TRAP), in rat lungs.Our results suggest that the acute use of corticosteroids is beneficial to lung tissue, whereas their chronic use is not.The chronic use of methylprednisolone appears to increase lung LPO levels.

View Article: PubMed Central - PubMed

Affiliation: Hospital Divina Providência, Porto Alegre, Brazil.

ABSTRACT

Objective: To determine the effects of acute and chronic administration of methylprednisolone on oxidative stress, as quantified by measuring lipid peroxidation (LPO) and total reactive antioxidant potential (TRAP), in rat lungs.

Methods: Forty Wistar rats were divided into four groups: acute treatment, comprising rats receiving a single injection of methylprednisolone (50 mg/kg i.p.); acute control, comprising rats i.p. injected with saline; chronic treatment, comprising rats receiving methylprednisolone in drinking water (6 mg/kg per day for 30 days); and chronic control, comprising rats receiving normal drinking water.

Results: The levels of TRAP were significantly higher in the acute treatment group rats than in the acute control rats, suggesting an improvement in the pulmonary defenses of the former. The levels of lung LPO were significantly higher in the chronic treatment group rats than in the chronic control rats, indicating oxidative damage in the lung tissue of the former.

Conclusions: Our results suggest that the acute use of corticosteroids is beneficial to lung tissue, whereas their chronic use is not. The chronic use of methylprednisolone appears to increase lung LPO levels.

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Related in: MedlinePlus

Média dos níveis de peroxidação lipídica nos pulmões de ratos submetidosa administração crônica de metilprednisolona oral (grupo de tratamentocrônico) ou não (grupo de controle crônico). QL: quimioluminescência; e cps:contagens por segundo. *p < 0,05, teste t de Student
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f08: Média dos níveis de peroxidação lipídica nos pulmões de ratos submetidosa administração crônica de metilprednisolona oral (grupo de tratamentocrônico) ou não (grupo de controle crônico). QL: quimioluminescência; e cps:contagens por segundo. *p < 0,05, teste t de Student

Mentions: Não encontramos nenhuma diferença entre o grupo de tratamento crônico e o grupo decontrole crônico no tocante aos níveis totais de PART (p > 0,05; Figura 3). O grau de dano oxidativo pulmonar,medido por meio de quimioluminescência, foi significativamente (38%) maior no grupode tratamento crônico do que no grupo de controle crônico (p < 0,05; Figura 4).


Effects of acute and chronic administration of methylprednisolone on oxidative stress in rat lungs.

Torres RL, Torres IL, Laste G, Ferreira MB, Cardoso PF, Belló-Klein A - J Bras Pneumol (2014 May-Jun)

Média dos níveis de peroxidação lipídica nos pulmões de ratos submetidosa administração crônica de metilprednisolona oral (grupo de tratamentocrônico) ou não (grupo de controle crônico). QL: quimioluminescência; e cps:contagens por segundo. *p < 0,05, teste t de Student
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109195&req=5

f08: Média dos níveis de peroxidação lipídica nos pulmões de ratos submetidosa administração crônica de metilprednisolona oral (grupo de tratamentocrônico) ou não (grupo de controle crônico). QL: quimioluminescência; e cps:contagens por segundo. *p < 0,05, teste t de Student
Mentions: Não encontramos nenhuma diferença entre o grupo de tratamento crônico e o grupo decontrole crônico no tocante aos níveis totais de PART (p > 0,05; Figura 3). O grau de dano oxidativo pulmonar,medido por meio de quimioluminescência, foi significativamente (38%) maior no grupode tratamento crônico do que no grupo de controle crônico (p < 0,05; Figura 4).

Bottom Line: To determine the effects of acute and chronic administration of methylprednisolone on oxidative stress, as quantified by measuring lipid peroxidation (LPO) and total reactive antioxidant potential (TRAP), in rat lungs.Our results suggest that the acute use of corticosteroids is beneficial to lung tissue, whereas their chronic use is not.The chronic use of methylprednisolone appears to increase lung LPO levels.

View Article: PubMed Central - PubMed

Affiliation: Hospital Divina Providência, Porto Alegre, Brazil.

ABSTRACT

Objective: To determine the effects of acute and chronic administration of methylprednisolone on oxidative stress, as quantified by measuring lipid peroxidation (LPO) and total reactive antioxidant potential (TRAP), in rat lungs.

Methods: Forty Wistar rats were divided into four groups: acute treatment, comprising rats receiving a single injection of methylprednisolone (50 mg/kg i.p.); acute control, comprising rats i.p. injected with saline; chronic treatment, comprising rats receiving methylprednisolone in drinking water (6 mg/kg per day for 30 days); and chronic control, comprising rats receiving normal drinking water.

Results: The levels of TRAP were significantly higher in the acute treatment group rats than in the acute control rats, suggesting an improvement in the pulmonary defenses of the former. The levels of lung LPO were significantly higher in the chronic treatment group rats than in the chronic control rats, indicating oxidative damage in the lung tissue of the former.

Conclusions: Our results suggest that the acute use of corticosteroids is beneficial to lung tissue, whereas their chronic use is not. The chronic use of methylprednisolone appears to increase lung LPO levels.

Show MeSH
Related in: MedlinePlus