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Effects of acute and chronic administration of methylprednisolone on oxidative stress in rat lungs.

Torres RL, Torres IL, Laste G, Ferreira MB, Cardoso PF, Belló-Klein A - J Bras Pneumol (2014 May-Jun)

Bottom Line: To determine the effects of acute and chronic administration of methylprednisolone on oxidative stress, as quantified by measuring lipid peroxidation (LPO) and total reactive antioxidant potential (TRAP), in rat lungs.Our results suggest that the acute use of corticosteroids is beneficial to lung tissue, whereas their chronic use is not.The chronic use of methylprednisolone appears to increase lung LPO levels.

View Article: PubMed Central - PubMed

Affiliation: Hospital Divina Providência, Porto Alegre, Brazil.

ABSTRACT

Objective: To determine the effects of acute and chronic administration of methylprednisolone on oxidative stress, as quantified by measuring lipid peroxidation (LPO) and total reactive antioxidant potential (TRAP), in rat lungs.

Methods: Forty Wistar rats were divided into four groups: acute treatment, comprising rats receiving a single injection of methylprednisolone (50 mg/kg i.p.); acute control, comprising rats i.p. injected with saline; chronic treatment, comprising rats receiving methylprednisolone in drinking water (6 mg/kg per day for 30 days); and chronic control, comprising rats receiving normal drinking water.

Results: The levels of TRAP were significantly higher in the acute treatment group rats than in the acute control rats, suggesting an improvement in the pulmonary defenses of the former. The levels of lung LPO were significantly higher in the chronic treatment group rats than in the chronic control rats, indicating oxidative damage in the lung tissue of the former.

Conclusions: Our results suggest that the acute use of corticosteroids is beneficial to lung tissue, whereas their chronic use is not. The chronic use of methylprednisolone appears to increase lung LPO levels.

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Related in: MedlinePlus

Média dos níveis de potencial antioxidante reativo total (PART) nospulmões de ratos submetidos a administração aguda de metilprednisolona(grupo de tratamento agudo) ou injeção de um volume igual de solução salina(grupo de controle agudo). Trolox: ácido2-carboxílico-6-hidroxi-2,5,7,8-tetrametilcromano. *p < 0,05, teste t deStudent.
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f05: Média dos níveis de potencial antioxidante reativo total (PART) nospulmões de ratos submetidos a administração aguda de metilprednisolona(grupo de tratamento agudo) ou injeção de um volume igual de solução salina(grupo de controle agudo). Trolox: ácido2-carboxílico-6-hidroxi-2,5,7,8-tetrametilcromano. *p < 0,05, teste t deStudent.

Mentions: Primeiramente avaliamos o efeito do tratamento agudo com metilprednisolona sobre osníveis de PART e POL em pulmões de ratos. Foi observado um aumento significativo (de20%) dos níveis totais de PART no grupo tratado (p < 0,05; Figura 1). Não houve diferença significativa entre os gruposquanto aos níveis de POL (p > 0,05; Figura2).


Effects of acute and chronic administration of methylprednisolone on oxidative stress in rat lungs.

Torres RL, Torres IL, Laste G, Ferreira MB, Cardoso PF, Belló-Klein A - J Bras Pneumol (2014 May-Jun)

Média dos níveis de potencial antioxidante reativo total (PART) nospulmões de ratos submetidos a administração aguda de metilprednisolona(grupo de tratamento agudo) ou injeção de um volume igual de solução salina(grupo de controle agudo). Trolox: ácido2-carboxílico-6-hidroxi-2,5,7,8-tetrametilcromano. *p < 0,05, teste t deStudent.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109195&req=5

f05: Média dos níveis de potencial antioxidante reativo total (PART) nospulmões de ratos submetidos a administração aguda de metilprednisolona(grupo de tratamento agudo) ou injeção de um volume igual de solução salina(grupo de controle agudo). Trolox: ácido2-carboxílico-6-hidroxi-2,5,7,8-tetrametilcromano. *p < 0,05, teste t deStudent.
Mentions: Primeiramente avaliamos o efeito do tratamento agudo com metilprednisolona sobre osníveis de PART e POL em pulmões de ratos. Foi observado um aumento significativo (de20%) dos níveis totais de PART no grupo tratado (p < 0,05; Figura 1). Não houve diferença significativa entre os gruposquanto aos níveis de POL (p > 0,05; Figura2).

Bottom Line: To determine the effects of acute and chronic administration of methylprednisolone on oxidative stress, as quantified by measuring lipid peroxidation (LPO) and total reactive antioxidant potential (TRAP), in rat lungs.Our results suggest that the acute use of corticosteroids is beneficial to lung tissue, whereas their chronic use is not.The chronic use of methylprednisolone appears to increase lung LPO levels.

View Article: PubMed Central - PubMed

Affiliation: Hospital Divina Providência, Porto Alegre, Brazil.

ABSTRACT

Objective: To determine the effects of acute and chronic administration of methylprednisolone on oxidative stress, as quantified by measuring lipid peroxidation (LPO) and total reactive antioxidant potential (TRAP), in rat lungs.

Methods: Forty Wistar rats were divided into four groups: acute treatment, comprising rats receiving a single injection of methylprednisolone (50 mg/kg i.p.); acute control, comprising rats i.p. injected with saline; chronic treatment, comprising rats receiving methylprednisolone in drinking water (6 mg/kg per day for 30 days); and chronic control, comprising rats receiving normal drinking water.

Results: The levels of TRAP were significantly higher in the acute treatment group rats than in the acute control rats, suggesting an improvement in the pulmonary defenses of the former. The levels of lung LPO were significantly higher in the chronic treatment group rats than in the chronic control rats, indicating oxidative damage in the lung tissue of the former.

Conclusions: Our results suggest that the acute use of corticosteroids is beneficial to lung tissue, whereas their chronic use is not. The chronic use of methylprednisolone appears to increase lung LPO levels.

Show MeSH
Related in: MedlinePlus