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Effects of acute and chronic administration of methylprednisolone on oxidative stress in rat lungs.

Torres RL, Torres IL, Laste G, Ferreira MB, Cardoso PF, Belló-Klein A - J Bras Pneumol (2014 May-Jun)

Bottom Line: To determine the effects of acute and chronic administration of methylprednisolone on oxidative stress, as quantified by measuring lipid peroxidation (LPO) and total reactive antioxidant potential (TRAP), in rat lungs.Our results suggest that the acute use of corticosteroids is beneficial to lung tissue, whereas their chronic use is not.The chronic use of methylprednisolone appears to increase lung LPO levels.

View Article: PubMed Central - PubMed

Affiliation: Hospital Divina Providência, Porto Alegre, Brazil.

ABSTRACT

Objective: To determine the effects of acute and chronic administration of methylprednisolone on oxidative stress, as quantified by measuring lipid peroxidation (LPO) and total reactive antioxidant potential (TRAP), in rat lungs.

Methods: Forty Wistar rats were divided into four groups: acute treatment, comprising rats receiving a single injection of methylprednisolone (50 mg/kg i.p.); acute control, comprising rats i.p. injected with saline; chronic treatment, comprising rats receiving methylprednisolone in drinking water (6 mg/kg per day for 30 days); and chronic control, comprising rats receiving normal drinking water.

Results: The levels of TRAP were significantly higher in the acute treatment group rats than in the acute control rats, suggesting an improvement in the pulmonary defenses of the former. The levels of lung LPO were significantly higher in the chronic treatment group rats than in the chronic control rats, indicating oxidative damage in the lung tissue of the former.

Conclusions: Our results suggest that the acute use of corticosteroids is beneficial to lung tissue, whereas their chronic use is not. The chronic use of methylprednisolone appears to increase lung LPO levels.

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Related in: MedlinePlus

Mean levels of total reactive antioxidant potential (TRAP) in the lungs ofrats subjected to acute administration of methylprednisolone (acute treatmentgroup) or injected with an equal volume of saline (acute control group).Trolox: 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid. *p < 0.05,Student's t-test.
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f01: Mean levels of total reactive antioxidant potential (TRAP) in the lungs ofrats subjected to acute administration of methylprednisolone (acute treatmentgroup) or injected with an equal volume of saline (acute control group).Trolox: 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid. *p < 0.05,Student's t-test.

Mentions: We first evaluated the effect of acute treatment with methylprednisolone on the levelsof TRAP and LPO in rat lungs. A significant (20%) increase was observed in total TRAPlevels in the treated group (p < 0.05; Figure1). No significant difference was found between the groups regarding LPO levels(p > 0.05; Figure 2).


Effects of acute and chronic administration of methylprednisolone on oxidative stress in rat lungs.

Torres RL, Torres IL, Laste G, Ferreira MB, Cardoso PF, Belló-Klein A - J Bras Pneumol (2014 May-Jun)

Mean levels of total reactive antioxidant potential (TRAP) in the lungs ofrats subjected to acute administration of methylprednisolone (acute treatmentgroup) or injected with an equal volume of saline (acute control group).Trolox: 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid. *p < 0.05,Student's t-test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109195&req=5

f01: Mean levels of total reactive antioxidant potential (TRAP) in the lungs ofrats subjected to acute administration of methylprednisolone (acute treatmentgroup) or injected with an equal volume of saline (acute control group).Trolox: 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid. *p < 0.05,Student's t-test.
Mentions: We first evaluated the effect of acute treatment with methylprednisolone on the levelsof TRAP and LPO in rat lungs. A significant (20%) increase was observed in total TRAPlevels in the treated group (p < 0.05; Figure1). No significant difference was found between the groups regarding LPO levels(p > 0.05; Figure 2).

Bottom Line: To determine the effects of acute and chronic administration of methylprednisolone on oxidative stress, as quantified by measuring lipid peroxidation (LPO) and total reactive antioxidant potential (TRAP), in rat lungs.Our results suggest that the acute use of corticosteroids is beneficial to lung tissue, whereas their chronic use is not.The chronic use of methylprednisolone appears to increase lung LPO levels.

View Article: PubMed Central - PubMed

Affiliation: Hospital Divina Providência, Porto Alegre, Brazil.

ABSTRACT

Objective: To determine the effects of acute and chronic administration of methylprednisolone on oxidative stress, as quantified by measuring lipid peroxidation (LPO) and total reactive antioxidant potential (TRAP), in rat lungs.

Methods: Forty Wistar rats were divided into four groups: acute treatment, comprising rats receiving a single injection of methylprednisolone (50 mg/kg i.p.); acute control, comprising rats i.p. injected with saline; chronic treatment, comprising rats receiving methylprednisolone in drinking water (6 mg/kg per day for 30 days); and chronic control, comprising rats receiving normal drinking water.

Results: The levels of TRAP were significantly higher in the acute treatment group rats than in the acute control rats, suggesting an improvement in the pulmonary defenses of the former. The levels of lung LPO were significantly higher in the chronic treatment group rats than in the chronic control rats, indicating oxidative damage in the lung tissue of the former.

Conclusions: Our results suggest that the acute use of corticosteroids is beneficial to lung tissue, whereas their chronic use is not. The chronic use of methylprednisolone appears to increase lung LPO levels.

Show MeSH
Related in: MedlinePlus