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COI barcode versus morphological identification of Culex (Culex) (Diptera: Culicidae) species: a case study using samples from Argentina and Brazil.

Laurito M, Oliveira TM, Almirón WR, Sallum MA - Mem. Inst. Oswaldo Cruz (2013)

Bottom Line: Regarding the BCM criteria, the COI barcode successfully identified 69% of all species.The rest of the sequences, approximately 10%, 18% and 3%, remained as ambiguously, mis and unidentified, respectively.The COI barcode does not contain enough information to distinguish Culex (Cux.) species.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigaciones Biológicas y Tecnológicas, Centro de Investigaciones Entomológicas de Córdoba, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Argentina, Instituto de Investigaciones Biológicas y Tecnológicas, Centro de Investigaciones Entomológicas de Córdoba, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Córdoba, Argentina.

ABSTRACT
Sequences of the cytochrome c oxidase subunit I (COI) mitochondrial gene from adults of 22 Culex (Culex) species from Argentina and Brazil were employed to assess species identification and to test the usefulness of COI for barcoding using the best close match (BCM) algorithm. A pairwise Kimura two-parameter distance matrix including the mean intra and interspecific distances for 71 COI barcode sequences was constructed. Of the 12 COI lineages recovered in the Neighbour-joining topology, five confirmed recognised morphological species (Cx. acharistus, Cx. chidesteri, Cx. dolosus, Cx. lygrus and Cx. saltanensis) with intraspecific divergences lower than 1.75%. Cx. bilineatus is formally resurrected from the synonymy of Cx. dolosus. Cx. maxi , Cx. surinamensis and the Coronator group species included were clustered into an unresolved lineage. The intraspecific distance of Cx. pipiens (3%) was almost twice the interspecific between it and Cx. quinquefasciatus (1.6%). Regarding the BCM criteria, the COI barcode successfully identified 69% of all species. The rest of the sequences, approximately 10%, 18% and 3%, remained as ambiguously, mis and unidentified, respectively. The COI barcode does not contain enough information to distinguish Culex (Cux.) species.

Show MeSH
: phylogenetic relationships of 71 cytochrome c oxidase subunit I (COI)sequences generated from specimens belonging to 22 Culex (Culex ) species from Argentina and Brazil based on themaximum parsimony criterion and 1,000 replicates. Anophelesdarlingi and Stegomyia aegypti were used asoutgroup. Bootstrap values less than 70% are not shown. The 12 COI lineagesrecovered are named.
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f02: : phylogenetic relationships of 71 cytochrome c oxidase subunit I (COI)sequences generated from specimens belonging to 22 Culex (Culex ) species from Argentina and Brazil based on themaximum parsimony criterion and 1,000 replicates. Anophelesdarlingi and Stegomyia aegypti were used asoutgroup. Bootstrap values less than 70% are not shown. The 12 COI lineagesrecovered are named.

Mentions: The MP tree topology ( Fig. 2 ) and the NJtopology ( Fig. 1 ) are consistent. The 12 COIlineages were recovered with both analyses with similar BSV.


COI barcode versus morphological identification of Culex (Culex) (Diptera: Culicidae) species: a case study using samples from Argentina and Brazil.

Laurito M, Oliveira TM, Almirón WR, Sallum MA - Mem. Inst. Oswaldo Cruz (2013)

: phylogenetic relationships of 71 cytochrome c oxidase subunit I (COI)sequences generated from specimens belonging to 22 Culex (Culex ) species from Argentina and Brazil based on themaximum parsimony criterion and 1,000 replicates. Anophelesdarlingi and Stegomyia aegypti were used asoutgroup. Bootstrap values less than 70% are not shown. The 12 COI lineagesrecovered are named.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109187&req=5

f02: : phylogenetic relationships of 71 cytochrome c oxidase subunit I (COI)sequences generated from specimens belonging to 22 Culex (Culex ) species from Argentina and Brazil based on themaximum parsimony criterion and 1,000 replicates. Anophelesdarlingi and Stegomyia aegypti were used asoutgroup. Bootstrap values less than 70% are not shown. The 12 COI lineagesrecovered are named.
Mentions: The MP tree topology ( Fig. 2 ) and the NJtopology ( Fig. 1 ) are consistent. The 12 COIlineages were recovered with both analyses with similar BSV.

Bottom Line: Regarding the BCM criteria, the COI barcode successfully identified 69% of all species.The rest of the sequences, approximately 10%, 18% and 3%, remained as ambiguously, mis and unidentified, respectively.The COI barcode does not contain enough information to distinguish Culex (Cux.) species.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigaciones Biológicas y Tecnológicas, Centro de Investigaciones Entomológicas de Córdoba, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Argentina, Instituto de Investigaciones Biológicas y Tecnológicas, Centro de Investigaciones Entomológicas de Córdoba, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Córdoba, Argentina.

ABSTRACT
Sequences of the cytochrome c oxidase subunit I (COI) mitochondrial gene from adults of 22 Culex (Culex) species from Argentina and Brazil were employed to assess species identification and to test the usefulness of COI for barcoding using the best close match (BCM) algorithm. A pairwise Kimura two-parameter distance matrix including the mean intra and interspecific distances for 71 COI barcode sequences was constructed. Of the 12 COI lineages recovered in the Neighbour-joining topology, five confirmed recognised morphological species (Cx. acharistus, Cx. chidesteri, Cx. dolosus, Cx. lygrus and Cx. saltanensis) with intraspecific divergences lower than 1.75%. Cx. bilineatus is formally resurrected from the synonymy of Cx. dolosus. Cx. maxi , Cx. surinamensis and the Coronator group species included were clustered into an unresolved lineage. The intraspecific distance of Cx. pipiens (3%) was almost twice the interspecific between it and Cx. quinquefasciatus (1.6%). Regarding the BCM criteria, the COI barcode successfully identified 69% of all species. The rest of the sequences, approximately 10%, 18% and 3%, remained as ambiguously, mis and unidentified, respectively. The COI barcode does not contain enough information to distinguish Culex (Cux.) species.

Show MeSH