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COI barcode versus morphological identification of Culex (Culex) (Diptera: Culicidae) species: a case study using samples from Argentina and Brazil.

Laurito M, Oliveira TM, Almirón WR, Sallum MA - Mem. Inst. Oswaldo Cruz (2013)

Bottom Line: Regarding the BCM criteria, the COI barcode successfully identified 69% of all species.The rest of the sequences, approximately 10%, 18% and 3%, remained as ambiguously, mis and unidentified, respectively.The COI barcode does not contain enough information to distinguish Culex (Cux.) species.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigaciones Biológicas y Tecnológicas, Centro de Investigaciones Entomológicas de Córdoba, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Argentina, Instituto de Investigaciones Biológicas y Tecnológicas, Centro de Investigaciones Entomológicas de Córdoba, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Córdoba, Argentina.

ABSTRACT
Sequences of the cytochrome c oxidase subunit I (COI) mitochondrial gene from adults of 22 Culex (Culex) species from Argentina and Brazil were employed to assess species identification and to test the usefulness of COI for barcoding using the best close match (BCM) algorithm. A pairwise Kimura two-parameter distance matrix including the mean intra and interspecific distances for 71 COI barcode sequences was constructed. Of the 12 COI lineages recovered in the Neighbour-joining topology, five confirmed recognised morphological species (Cx. acharistus, Cx. chidesteri, Cx. dolosus, Cx. lygrus and Cx. saltanensis) with intraspecific divergences lower than 1.75%. Cx. bilineatus is formally resurrected from the synonymy of Cx. dolosus. Cx. maxi , Cx. surinamensis and the Coronator group species included were clustered into an unresolved lineage. The intraspecific distance of Cx. pipiens (3%) was almost twice the interspecific between it and Cx. quinquefasciatus (1.6%). Regarding the BCM criteria, the COI barcode successfully identified 69% of all species. The rest of the sequences, approximately 10%, 18% and 3%, remained as ambiguously, mis and unidentified, respectively. The COI barcode does not contain enough information to distinguish Culex (Cux.) species.

Show MeSH
: bootstrapped neighbour-joining tree of 71 cytochrome c oxidase subunit I(COI) sequences generated from specimens belonging to 22 Culex( Culex ) species from Argentina and Brazil based on theKimura two-parameter distance algorithm and 1,000 replicates. Bootstrap valuesless than 70% are not shown. The 12 COI lineages recovered are named.
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f01: : bootstrapped neighbour-joining tree of 71 cytochrome c oxidase subunit I(COI) sequences generated from specimens belonging to 22 Culex( Culex ) species from Argentina and Brazil based on theKimura two-parameter distance algorithm and 1,000 replicates. Bootstrap valuesless than 70% are not shown. The 12 COI lineages recovered are named.

Mentions: Barcode clusters - A pairwise K2P distance matrix was constructed forthe 71 COI barcode sequences. K2P distances and means, between and within groups, are inSupplementary data 2, Table II , respectively.Twelve COI lineages were recovered ( Fig. 1 ),which did not entirely corroborate previously identified species and subgroups.


COI barcode versus morphological identification of Culex (Culex) (Diptera: Culicidae) species: a case study using samples from Argentina and Brazil.

Laurito M, Oliveira TM, Almirón WR, Sallum MA - Mem. Inst. Oswaldo Cruz (2013)

: bootstrapped neighbour-joining tree of 71 cytochrome c oxidase subunit I(COI) sequences generated from specimens belonging to 22 Culex( Culex ) species from Argentina and Brazil based on theKimura two-parameter distance algorithm and 1,000 replicates. Bootstrap valuesless than 70% are not shown. The 12 COI lineages recovered are named.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109187&req=5

f01: : bootstrapped neighbour-joining tree of 71 cytochrome c oxidase subunit I(COI) sequences generated from specimens belonging to 22 Culex( Culex ) species from Argentina and Brazil based on theKimura two-parameter distance algorithm and 1,000 replicates. Bootstrap valuesless than 70% are not shown. The 12 COI lineages recovered are named.
Mentions: Barcode clusters - A pairwise K2P distance matrix was constructed forthe 71 COI barcode sequences. K2P distances and means, between and within groups, are inSupplementary data 2, Table II , respectively.Twelve COI lineages were recovered ( Fig. 1 ),which did not entirely corroborate previously identified species and subgroups.

Bottom Line: Regarding the BCM criteria, the COI barcode successfully identified 69% of all species.The rest of the sequences, approximately 10%, 18% and 3%, remained as ambiguously, mis and unidentified, respectively.The COI barcode does not contain enough information to distinguish Culex (Cux.) species.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigaciones Biológicas y Tecnológicas, Centro de Investigaciones Entomológicas de Córdoba, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Argentina, Instituto de Investigaciones Biológicas y Tecnológicas, Centro de Investigaciones Entomológicas de Córdoba, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Córdoba, Argentina.

ABSTRACT
Sequences of the cytochrome c oxidase subunit I (COI) mitochondrial gene from adults of 22 Culex (Culex) species from Argentina and Brazil were employed to assess species identification and to test the usefulness of COI for barcoding using the best close match (BCM) algorithm. A pairwise Kimura two-parameter distance matrix including the mean intra and interspecific distances for 71 COI barcode sequences was constructed. Of the 12 COI lineages recovered in the Neighbour-joining topology, five confirmed recognised morphological species (Cx. acharistus, Cx. chidesteri, Cx. dolosus, Cx. lygrus and Cx. saltanensis) with intraspecific divergences lower than 1.75%. Cx. bilineatus is formally resurrected from the synonymy of Cx. dolosus. Cx. maxi , Cx. surinamensis and the Coronator group species included were clustered into an unresolved lineage. The intraspecific distance of Cx. pipiens (3%) was almost twice the interspecific between it and Cx. quinquefasciatus (1.6%). Regarding the BCM criteria, the COI barcode successfully identified 69% of all species. The rest of the sequences, approximately 10%, 18% and 3%, remained as ambiguously, mis and unidentified, respectively. The COI barcode does not contain enough information to distinguish Culex (Cux.) species.

Show MeSH