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Mosquitoes of eastern Amazonian Ecuador: biodiversity, bionomics and barcodes.

Linton YM, Pecor JE, Porter CH, Mitchell LB, Garzón-Moreno A, Foley DH, Pecor DB, Wilkerson RC - Mem. Inst. Oswaldo Cruz (2013)

Bottom Line: A total of 2,284 specimens were preserved; 1,671 specimens were link-reared with associated immature exuviae, all but 108 of which are slide mounted.DNA barcoding proved useful in uncovering and confirming new species and we advocate an integrated systematics approach to biodiversity studies in future.An updated systematic checklist of the mosquitoes of Ecuador (n=179) is presented for the first time in 60 years.

View Article: PubMed Central - PubMed

Affiliation: Walter Reed Army Institute of Research, Entomology Branch, USA, Silver SpringMD, Walter Reed Army Institute of Research, Entomology Branch, Silver Spring, MD, USA.

ABSTRACT
Two snapshot surveys to establish the diversity and ecological preferences of mosquitoes (Diptera: Culicidae) in the terra firme primary rain forest surrounding the Tiputini Biodiversity Station in the UNESCO Yasuní Biosphere Reserve of eastern Amazonian Ecuador were carried out in November 1998 and May 1999. The mosquito fauna of this region is poorly known; the focus of this study was to obtain high quality link-reared specimens that could be used to unequivocally confirm species level diversity through integrated systematic study of all life stages and DNA sequences. A total of 2,284 specimens were preserved; 1,671 specimens were link-reared with associated immature exuviae, all but 108 of which are slide mounted. This study identified 68 unique taxa belonging to 17 genera and 27 subgenera. Of these, 12 are new to science and 37 comprise new country records. DNA barcodes [658-bp of the mtDNA cytochrome c oxidase (COI) I gene] are presented for 58 individuals representing 20 species and nine genera. DNA barcoding proved useful in uncovering and confirming new species and we advocate an integrated systematics approach to biodiversity studies in future. Associated bionomics of all species collected are discussed. An updated systematic checklist of the mosquitoes of Ecuador (n=179) is presented for the first time in 60 years.

Show MeSH
: mosquito taxa identified from forest habitat in the Ecuadorian Amazon,detailing diversity in larvae habitats and adult collections methodology. Some68 unique taxa, representing 17 genera and 27 subgenera and 12 species new toscience, were collected in this study. Two undetermined species representingnew subgeneric records for Anopheles  (Stethomyia ) and Sabethes  (Sabethes ) are also included. Subgeneric abbreviations aregiven in parenthesis. Anopheles  sp. nr.  konderi is the same taxa as in Ruiz-Lopez et al. (2013). Undetermined specieswithin the Culex coronator group: males of Cx.camposi and Cx. usquatus have been unequivocallyconfirmed using slide-mounted genitalia and are listed separately.
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f03: : mosquito taxa identified from forest habitat in the Ecuadorian Amazon,detailing diversity in larvae habitats and adult collections methodology. Some68 unique taxa, representing 17 genera and 27 subgenera and 12 species new toscience, were collected in this study. Two undetermined species representingnew subgeneric records for Anopheles (Stethomyia ) and Sabethes (Sabethes ) are also included. Subgeneric abbreviations aregiven in parenthesis. Anopheles sp. nr. konderi is the same taxa as in Ruiz-Lopez et al. (2013). Undetermined specieswithin the Culex coronator group: males of Cx.camposi and Cx. usquatus have been unequivocallyconfirmed using slide-mounted genitalia and are listed separately.

Mentions: Specimen collection - Mosquito collections were carried out in a rangeof natural and artificial habitats in the forest environment within a 10 kmcircumference of the TBS, Yasuní Biosphere Reserve and National Park, Orellana province,Ecuador (Fig. S1).


Mosquitoes of eastern Amazonian Ecuador: biodiversity, bionomics and barcodes.

Linton YM, Pecor JE, Porter CH, Mitchell LB, Garzón-Moreno A, Foley DH, Pecor DB, Wilkerson RC - Mem. Inst. Oswaldo Cruz (2013)

: mosquito taxa identified from forest habitat in the Ecuadorian Amazon,detailing diversity in larvae habitats and adult collections methodology. Some68 unique taxa, representing 17 genera and 27 subgenera and 12 species new toscience, were collected in this study. Two undetermined species representingnew subgeneric records for Anopheles  (Stethomyia ) and Sabethes  (Sabethes ) are also included. Subgeneric abbreviations aregiven in parenthesis. Anopheles  sp. nr.  konderi is the same taxa as in Ruiz-Lopez et al. (2013). Undetermined specieswithin the Culex coronator group: males of Cx.camposi and Cx. usquatus have been unequivocallyconfirmed using slide-mounted genitalia and are listed separately.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109186&req=5

f03: : mosquito taxa identified from forest habitat in the Ecuadorian Amazon,detailing diversity in larvae habitats and adult collections methodology. Some68 unique taxa, representing 17 genera and 27 subgenera and 12 species new toscience, were collected in this study. Two undetermined species representingnew subgeneric records for Anopheles (Stethomyia ) and Sabethes (Sabethes ) are also included. Subgeneric abbreviations aregiven in parenthesis. Anopheles sp. nr. konderi is the same taxa as in Ruiz-Lopez et al. (2013). Undetermined specieswithin the Culex coronator group: males of Cx.camposi and Cx. usquatus have been unequivocallyconfirmed using slide-mounted genitalia and are listed separately.
Mentions: Specimen collection - Mosquito collections were carried out in a rangeof natural and artificial habitats in the forest environment within a 10 kmcircumference of the TBS, Yasuní Biosphere Reserve and National Park, Orellana province,Ecuador (Fig. S1).

Bottom Line: A total of 2,284 specimens were preserved; 1,671 specimens were link-reared with associated immature exuviae, all but 108 of which are slide mounted.DNA barcoding proved useful in uncovering and confirming new species and we advocate an integrated systematics approach to biodiversity studies in future.An updated systematic checklist of the mosquitoes of Ecuador (n=179) is presented for the first time in 60 years.

View Article: PubMed Central - PubMed

Affiliation: Walter Reed Army Institute of Research, Entomology Branch, USA, Silver SpringMD, Walter Reed Army Institute of Research, Entomology Branch, Silver Spring, MD, USA.

ABSTRACT
Two snapshot surveys to establish the diversity and ecological preferences of mosquitoes (Diptera: Culicidae) in the terra firme primary rain forest surrounding the Tiputini Biodiversity Station in the UNESCO Yasuní Biosphere Reserve of eastern Amazonian Ecuador were carried out in November 1998 and May 1999. The mosquito fauna of this region is poorly known; the focus of this study was to obtain high quality link-reared specimens that could be used to unequivocally confirm species level diversity through integrated systematic study of all life stages and DNA sequences. A total of 2,284 specimens were preserved; 1,671 specimens were link-reared with associated immature exuviae, all but 108 of which are slide mounted. This study identified 68 unique taxa belonging to 17 genera and 27 subgenera. Of these, 12 are new to science and 37 comprise new country records. DNA barcodes [658-bp of the mtDNA cytochrome c oxidase (COI) I gene] are presented for 58 individuals representing 20 species and nine genera. DNA barcoding proved useful in uncovering and confirming new species and we advocate an integrated systematics approach to biodiversity studies in future. Associated bionomics of all species collected are discussed. An updated systematic checklist of the mosquitoes of Ecuador (n=179) is presented for the first time in 60 years.

Show MeSH