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Rhodnius barretti, a new species of Triatominae (Hemiptera: Reduviidae) from western Amazonia.

Abad-Franch F, Pavan MG, Jaramillo-O N, Palomeque FS, Dale C, Chaverra D, Monteiro FA - Mem. Inst. Oswaldo Cruz (2013)

Bottom Line: Rhodnius barretti, a new triatomine species, is described based on adult specimens collected in rainforest environments within the Napo ecoregion of western Amazonia (Colombia and Ecuador).R. barretti resembles Rhodnius robustus s.l., but mitochondrial cytochrome b gene sequences reveal that it is a strongly divergent member of the "robustus lineage", i.e., basal to the clade encompassing Rhodnius nasutus, Rhodnius neglectus, Rhodnius prolixus and five members of the R. robustus species complex.R. barretti occurs, often at high densities, in Attalea butyracea and Oenocarpus bataua palms.

View Article: PubMed Central - PubMed

Affiliation: Instituto Leônidas e Maria Deane, Fiocruz, Brasil, ManausAM, Instituto Leônidas e Maria Deane-Fiocruz, Manaus, AM, Brasil.

ABSTRACT
Rhodnius barretti, a new triatomine species, is described based on adult specimens collected in rainforest environments within the Napo ecoregion of western Amazonia (Colombia and Ecuador). R. barretti resembles Rhodnius robustus s.l., but mitochondrial cytochrome b gene sequences reveal that it is a strongly divergent member of the "robustus lineage", i.e., basal to the clade encompassing Rhodnius nasutus, Rhodnius neglectus, Rhodnius prolixus and five members of the R. robustus species complex. Morphometric analyses also reveal consistent divergence from R. robustus s.l., including head and, as previously shown, wing shape and the length ratios of some anatomical structures. R. barretti occurs, often at high densities, in Attalea butyracea and Oenocarpus bataua palms. It is strikingly aggressive and adults may invade houses flying from peridomestic palms. R. barretti must therefore be regarded as a potential Trypanosoma cruzi vector in the Napo ecoregion, where Chagas disease is endemic.

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Related in: MedlinePlus

: bayesian consensus tree of 13 663 bp cyt bRhodnius sequences and a heatmap illustrating genetic distancevalues. Posterior probabilities > 0.95 are shown for key nodes. Squares inthe heatmap illustrate pairwise genetic distance values between sequences, withdarker colours representing larger distances (see scale bar). Collection sitesand GenBank accessions are: Rhodnius prolixus - Portuguesa,Venezuela, EF011723; Rhodnius robustus I - Trujillo,Venezuela, AF421340; R. robustus II - Napo, Ecuador, AF421341;R. robustus III - Pará (PA), Brazil, AF421342; R.robustus IV - PA, Brazil, AF421342; R. robustus V- Novo Airão, Amazonas (AM), Brazil, JX273158; Rhodniusnasutus - Ceará, Brazil, JX273155; Rhodniusneglectus - Tocantins, Brazil, JX273156; Rhodniuspictipes - AM, Brazil; Rhodnius barretti - LagoAgrio, Sucumbíos, Ecuador (1), JX273159; R. barretti - LagoAgrio, Sucumbíos, Ecuador (2), JX273160; R. barretti - PuertoAsís, Putumayo, Colombia (1 and 2), JX273161 and JX273162.
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f04: : bayesian consensus tree of 13 663 bp cyt bRhodnius sequences and a heatmap illustrating genetic distancevalues. Posterior probabilities > 0.95 are shown for key nodes. Squares inthe heatmap illustrate pairwise genetic distance values between sequences, withdarker colours representing larger distances (see scale bar). Collection sitesand GenBank accessions are: Rhodnius prolixus - Portuguesa,Venezuela, EF011723; Rhodnius robustus I - Trujillo,Venezuela, AF421340; R. robustus II - Napo, Ecuador, AF421341;R. robustus III - Pará (PA), Brazil, AF421342; R.robustus IV - PA, Brazil, AF421342; R. robustus V- Novo Airão, Amazonas (AM), Brazil, JX273158; Rhodniusnasutus - Ceará, Brazil, JX273155; Rhodniusneglectus - Tocantins, Brazil, JX273156; Rhodniuspictipes - AM, Brazil; Rhodnius barretti - LagoAgrio, Sucumbíos, Ecuador (1), JX273159; R. barretti - LagoAgrio, Sucumbíos, Ecuador (2), JX273160; R. barretti - PuertoAsís, Putumayo, Colombia (1 and 2), JX273161 and JX273162.

Mentions: Molecular analysis - The analysis of 13 sequences ofRhodnius for the cyt b gene fragments (663 bp)revealed 201 variable sites, 31 (15.4%) of which were autapomorphies of R.barretti . Three haplotypes were isolated from R. barrettispecimens (GenBank accessions JX273159-JX273162); Colombian bugs shared the same cytb haplotype, which closely resembled the other two, present inEcuador. The finding of three haplotypes in a sample of four bugs (mean distance = 0.3%;variance = 0.1%) is suggestive of high levels of genetic diversity. R.barretti cyt b sequences are unmistakably different fromthose of other “robustus lineage” members. Such deep divergence is shown in thephylogram presented in Fig. 4; all R. barretti specimens clustertogether in a 100%-supported clade that is basal to the rest of “robustus lineage”species, including R. nasutus and R. neglectus .Pairwise cyt b sequence divergence between R. barrettiand the other Rhodnius species, computed via M-CL, was very large,ranging from 7.4-10.7% (darker colours in the heatmap of Fig. 4 ). These values are larger than those between the four members of theR. robustus cryptic species complex and R. nasutusor between R. robustus I-V and R. neglectus . Thepairwise sequence divergence between Ecuadorian R. barretti andsympatric, field-collected R. robustus is 7.5%. Overall, the geneticdistance between R. robustus and R. barretti is 8.7%(variance = 2%; range, 7.4-9.7%). This is strong evidence that R.barretti represents a distinct entity within the “robustus lineage”.


Rhodnius barretti, a new species of Triatominae (Hemiptera: Reduviidae) from western Amazonia.

Abad-Franch F, Pavan MG, Jaramillo-O N, Palomeque FS, Dale C, Chaverra D, Monteiro FA - Mem. Inst. Oswaldo Cruz (2013)

: bayesian consensus tree of 13 663 bp cyt bRhodnius sequences and a heatmap illustrating genetic distancevalues. Posterior probabilities > 0.95 are shown for key nodes. Squares inthe heatmap illustrate pairwise genetic distance values between sequences, withdarker colours representing larger distances (see scale bar). Collection sitesand GenBank accessions are: Rhodnius prolixus - Portuguesa,Venezuela, EF011723; Rhodnius robustus I - Trujillo,Venezuela, AF421340; R. robustus II - Napo, Ecuador, AF421341;R. robustus III - Pará (PA), Brazil, AF421342; R.robustus IV - PA, Brazil, AF421342; R. robustus V- Novo Airão, Amazonas (AM), Brazil, JX273158; Rhodniusnasutus - Ceará, Brazil, JX273155; Rhodniusneglectus - Tocantins, Brazil, JX273156; Rhodniuspictipes - AM, Brazil; Rhodnius barretti - LagoAgrio, Sucumbíos, Ecuador (1), JX273159; R. barretti - LagoAgrio, Sucumbíos, Ecuador (2), JX273160; R. barretti - PuertoAsís, Putumayo, Colombia (1 and 2), JX273161 and JX273162.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109185&req=5

f04: : bayesian consensus tree of 13 663 bp cyt bRhodnius sequences and a heatmap illustrating genetic distancevalues. Posterior probabilities > 0.95 are shown for key nodes. Squares inthe heatmap illustrate pairwise genetic distance values between sequences, withdarker colours representing larger distances (see scale bar). Collection sitesand GenBank accessions are: Rhodnius prolixus - Portuguesa,Venezuela, EF011723; Rhodnius robustus I - Trujillo,Venezuela, AF421340; R. robustus II - Napo, Ecuador, AF421341;R. robustus III - Pará (PA), Brazil, AF421342; R.robustus IV - PA, Brazil, AF421342; R. robustus V- Novo Airão, Amazonas (AM), Brazil, JX273158; Rhodniusnasutus - Ceará, Brazil, JX273155; Rhodniusneglectus - Tocantins, Brazil, JX273156; Rhodniuspictipes - AM, Brazil; Rhodnius barretti - LagoAgrio, Sucumbíos, Ecuador (1), JX273159; R. barretti - LagoAgrio, Sucumbíos, Ecuador (2), JX273160; R. barretti - PuertoAsís, Putumayo, Colombia (1 and 2), JX273161 and JX273162.
Mentions: Molecular analysis - The analysis of 13 sequences ofRhodnius for the cyt b gene fragments (663 bp)revealed 201 variable sites, 31 (15.4%) of which were autapomorphies of R.barretti . Three haplotypes were isolated from R. barrettispecimens (GenBank accessions JX273159-JX273162); Colombian bugs shared the same cytb haplotype, which closely resembled the other two, present inEcuador. The finding of three haplotypes in a sample of four bugs (mean distance = 0.3%;variance = 0.1%) is suggestive of high levels of genetic diversity. R.barretti cyt b sequences are unmistakably different fromthose of other “robustus lineage” members. Such deep divergence is shown in thephylogram presented in Fig. 4; all R. barretti specimens clustertogether in a 100%-supported clade that is basal to the rest of “robustus lineage”species, including R. nasutus and R. neglectus .Pairwise cyt b sequence divergence between R. barrettiand the other Rhodnius species, computed via M-CL, was very large,ranging from 7.4-10.7% (darker colours in the heatmap of Fig. 4 ). These values are larger than those between the four members of theR. robustus cryptic species complex and R. nasutusor between R. robustus I-V and R. neglectus . Thepairwise sequence divergence between Ecuadorian R. barretti andsympatric, field-collected R. robustus is 7.5%. Overall, the geneticdistance between R. robustus and R. barretti is 8.7%(variance = 2%; range, 7.4-9.7%). This is strong evidence that R.barretti represents a distinct entity within the “robustus lineage”.

Bottom Line: Rhodnius barretti, a new triatomine species, is described based on adult specimens collected in rainforest environments within the Napo ecoregion of western Amazonia (Colombia and Ecuador).R. barretti resembles Rhodnius robustus s.l., but mitochondrial cytochrome b gene sequences reveal that it is a strongly divergent member of the "robustus lineage", i.e., basal to the clade encompassing Rhodnius nasutus, Rhodnius neglectus, Rhodnius prolixus and five members of the R. robustus species complex.R. barretti occurs, often at high densities, in Attalea butyracea and Oenocarpus bataua palms.

View Article: PubMed Central - PubMed

Affiliation: Instituto Leônidas e Maria Deane, Fiocruz, Brasil, ManausAM, Instituto Leônidas e Maria Deane-Fiocruz, Manaus, AM, Brasil.

ABSTRACT
Rhodnius barretti, a new triatomine species, is described based on adult specimens collected in rainforest environments within the Napo ecoregion of western Amazonia (Colombia and Ecuador). R. barretti resembles Rhodnius robustus s.l., but mitochondrial cytochrome b gene sequences reveal that it is a strongly divergent member of the "robustus lineage", i.e., basal to the clade encompassing Rhodnius nasutus, Rhodnius neglectus, Rhodnius prolixus and five members of the R. robustus species complex. Morphometric analyses also reveal consistent divergence from R. robustus s.l., including head and, as previously shown, wing shape and the length ratios of some anatomical structures. R. barretti occurs, often at high densities, in Attalea butyracea and Oenocarpus bataua palms. It is strikingly aggressive and adults may invade houses flying from peridomestic palms. R. barretti must therefore be regarded as a potential Trypanosoma cruzi vector in the Napo ecoregion, where Chagas disease is endemic.

Show MeSH
Related in: MedlinePlus